EFFECT OF RUTIN AGAINST HIGH-FAT DIET AND ALCOHOL-INDUCED ALTERATIONS IN HEMATOLOGICAL VARIABLES OF RATS
Objectives: Alcohol abuse leads to several diseases and millions of death worldwide. High-fat diet (HFD) is major contributor of non-alcoholic liver diseases and obesity. Combined consumption of HFD and alcohol has deleterious effect on blood cells. This study was carried out to evaluate the protective effect of rutin against combined consumption of HFD and alcohol-induced hematological alterations.
Methods: HFD 30% and ethanol 10% were administered for 4 weeks for induction of toxicity. Rutin (10, 20, and 40 mg/kg) and 50 mg/kg dose of silymarin were administered along with HFD and alcohol for 4 weeks.
Results: Combined consumption of HFD and alcohol increased mean corpuscular volume, total leukocytes count, eosinophil and monocyte, and decreased hematocrit and platelets. Administration of rutin improved hematological variables altered by HFD and alcohol consumption.
Conclusion: The present study concluded that administration of rutin may alleviate HFD and alcohol-induced hematological alterations by scavenging free radicals generation.
2. World Health Organization. Global Status Report on Alcohol and Health. Geneva: World Health Organization; 2014.
3. Leung TM, Nieto N. CYP2E1 and oxidant stress in alcoholic and non alcoholic fatty liver disease. J Hepatol 2013;58:395-8.
4. Ying HZ, Liu YH, Yu B, Wang ZY, Zang JN, Yu CH. Dietary Quercetin ameliorates nonalcoholic steatohepatitis induced by a high fat diet in gerbils. Food Chem Toxicol 2013;52:53-60.
5. Ushida Y, Matsui T, Tanaka M, Matsumoto K, Hosoyama H, Mitomi A, et al. Endothelium-dependent vasorelaxation effect of rutin-free tartary buckwheat extract in isolated rat thoracic aorta. J Nutr Biochem 2008;19:700-7.
6. Korkmaz A, Kolankaya D. Protective effect of rutin on the ischemia reperfusion induced damage in rat kidney. J Surg Res 2010;164:309-15.
7. Janbaz KH, Saeed SA, Gilani AH. Protective effect of rutin on paracetamol-and CCl4-induced hepatotoxicity in rodents. Fitoterapia 2002;73:557-63.
8. Lee W, Ku SK, Bae JS. Barrier protective effects of rutin in LPS-induced inflammation in vitro and in vivo. Food Chem Toxicol 2012;50:3048-55.
9. Snedecor GW, Cochran WG. Statistical Method. 8th ed. Ames, IA: Affiliated East-West Press; 1989. p. 217-36.
10. Yoganandh T, Mayilsamy S, Radhakrishnan S. Comparison of haematological parameters between alcoholics and non-alcoholics. Int J Res Med Sci 2017;5 Suppl 11:5041-7.
11. Das SK, Mukherjee S, Vasudevan DM. Non-alcoholic fatty liver diseases: An under recognized cause with emerging importance. Current Sci 2006;90:659-65.
12. Abozid MM, Zein H, El-Halem AA. Effect of common carp and African catfish oils on rats fed on high-fat diet. Int J Pharm Pharm Sci 2018;10 Suppl 2:96-101.
13. Ganong WF. Review of Medical Physiology. 21st ed. New York: McGraw-Hill; 2003. p. 518.
14. Sembulingam K, Sembulingam P. Essentials of Medical Physiology. 4th ed. New Delhi, India: Jaypee Brothers Medical Publishers; 2006. p. 47-126.
15. Hernandez-Munoz R, Montical-Ruiz C, Vazopen-Martiny O. Gastric mucosal cell proliferation in ethanol-induced chronic mucosal injury is related to oxidative stress and lipid peroxidation in rats. Lab Invest 2000;80:1161-9.
16. Ballard HS. The hematological complications of alcoholism. Alcohol Health Res World 1997;21:42-52.
17. Devi J, Rajkumar J. Effect of ambrex (a herbal formulation) on hematological variables in hyperlipidemic rats. Pak J Biol Sci 2014;17 Suppl 5:740-3.
18. Ogasawara F, Fusegawa H, Haruki Y, Shiraishi K, Watanabe N, Matsuzaki S, et al. Platelet activation in patients with alcoholic liver disease. Tokai J Exp Clin Med 2005;30:41-8.
19. Aarts PA, Bolhuis PA, Sakariassen KS, Heethaar RM, Sixma JJ. Red blood cell size is important for adherence of blood platelets to artery sub endothelium. Blood 1983;62:214-7.
20. NiemelÃ¤ O. Serum diagnosis of alcoholic liver disease and markers of ethanol intake. In: Sherman DIN, Preedy VR, Watson RR, editors. Ethanol and Liver. Mechanisms and Management. London: Taylor and Francis; 2002. p. 411-49.
21. Levine RF, Spivak JL, Meagher RC, Sieber F. Effect of ethanol on thrombopoiesis. Br J Haematol 1986;62:345-54.
22. Mahmoud AM. Hematological alterations in diabetic rats-role of adipocytokines and effect of citrus flavonoids. EXCLI J 2013;12:647 57.
23. Parabathina RK, Muralinath E, Swamy PL, Krishna VV, Sree KS. Effects of vitamin-E, Morin, Rutin, Quercetin AGAINST doxorubicin in rabbits: A hematological study. Res J Pharm Biol Chem Sci 2011;2 Suppl 3:74-84.
24. Lindenbaum J. Hematologic complications of alcohol abuse. Semin Liver Dis 1987;7:169-81.
25. Chu YC. Hematological effect of alcohol, long-term ethanol consumption in alcoholics. Alcohol Clin Exp Res 2000;24:117-22.
26. Ohshita K, Yamane K, Hanafusa M, Mori H, Mito K, Okubo M. Elevated white blood cell count in subjects with impaired glucose tolerance. Diabetes Care 2004;27;491-6.
27. Lee CD, Folsom AR, Nieto FJ, Chambless LE, Shahar E, Wolfe DA. White blood cell count and incidence of coronary heart disease and ischemic stroke and mortality from cardiovascular disease in African-American and white men and women: Atherosclerosis risk in communities study. Am J Epidemiol 2001;154:758-64.
28. Tong PC, Lee KF, So WY, Ng MH, Chan WB, Lo MK. White blood cell count isassociated with macro and microvascular complications in Chinese patients with Type 2 diabetes. Diabetes Care 2005;27:216-22.
29. Pertynska-Marczewska M, Kiriakidis S, Wait R, Beech J, Feldmann M, Paleolog EM. Advanced glycation end products upregulate angiogenic and pro-inflammatory cytokine production in human monocyte/ macrophages. Cytokine 2004;28:35-47.
30. Shurtz-Swirski R, Sela S, Herskovits AT, Shasha SM, Shapiro G, Nasser L. Involvement of peripheral polymorphonuclear leukocytes in oxidative stress and inflammation in Type 2 diabetic patients. Diabetes Care 2004;24:104-10.
31. Lee P, Peng H, Gelbart T, Beutler E. TheIL-6- and lipopolysaccharideinduced transcription of hepcidin in HFE-, transferrin receptor 2-, and beta 2 microglobulin deficient hepatocytes. Proc Natl Acad Sci U S A 2004;101:9263-5.
32. Scherberich JE. Proinflammatory blood monocytes: Main effector and target cells in systemic and renal disease; Background and therapeutic implications. Int J Clin Pharmacol Ther 2003;41:459-64.
33. Bhalchandra W, Alqadhi YA, Ninawe AS. Ameliorative role of bee honey and royal jelly against cisplatin induced alteration in hematological parameters in male wister albino rat. Int J Pharm Pharm Sci 2018;10 Suppl 4:110-4.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.