PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ECLIPTA ALBA L.

  • Thenmozhi M Department of Biotechnology, Vels Insititute of Science, Technology and Advanced Studies, Pallavaram, Chennai, Tamil Nadu, India.
  • JAYANTHI M

Abstract

Objective: Earlier various studies on pharmacological and therapeutic properties of Eclipta alba L. have been reported. The present investigation is on phytochemical screening and antioxidant activity of E. alba L. using different solvent extracts.


Method: The shade-dried and grounded E. alba L. plant materials were treated with petroleum ether, chloroform, ethyl acetate, ethanol, methanol, and water (aqueous) solvents. The extracted residue was then dissolved in dimethylsulfoxide and further used for phytochemical screening and antioxidant studies.


Results: E. alba L. on different solvent extract, the extractive yield was higher in methanol (14.22±0.99% w/w). The phytochemical screening analysis showed high number of secondary metabolites in the aqueous extract compared to other solvent studied. The metabolite flavonoids, phenols, and tannins are likely to be antioxidant potential compound. Hence, further, the total flavonoid content determination showed high of 0.875±0.020 μg/g in methanol extract. Further antioxidant property of E. alba L. was studied by method such as1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, nitric oxide scavenging activity, metal chelating activity, and superoxide dismutase activity which showed high antioxidant potency with IC50 values of 62.76±0.63 μg/ml, 96.24±0.34 μg/ml, 72.29±0.61 μg/ml, and 108.40±0.52 μg/ml, respectively, in the petroleum ether extract.


Conclusion: From the present study, the substantiate result of antioxidant studies has been obtained from petroleum ether extract . Furthermore the extractive value and flavonoid content was observed to be high in methanol extract. The antioxidant potency of E. alba L. was found to be high in petroleum ether extract compared to other solvent studied.

Keywords: Eclipta alba L., Methanol, Antioxidant, Flavonoid, 1,1-diphenyl-2-picrylhydrazyl, Radical scavenging.

References

1. Selvamani S, Balamurugan S. In vitro antibacterial activity of Eclipta alba (L.) Hassk. Int Lett Nat Sci 2014;21:28-34.
2. Navneet KY, Rakesh KA, Kapil D, Chetan S, Zakir H, et al. Alcoholic extract of Eclipta alba L. Shows in vitro antioxidant and anticancer activity without exhibiting toxicological effects. Oxid Med Cell Longevity 2017;2017:1-18.
3. Regupathi T, Chitra K. In vitro antioxidant properties of Eclipta alba (L.) Hassk. And Lippia nodiflora Linn. Int J Pharm Phytopharm Res 2015;4:227-30.
4. Harborne JB. Phytochemical Methods. London: Chapman and Hall Ltd.; 1973. p. 49-188.
5. Harborne JB. Phytochemical Methods. London: Chapman and Hall Ltd.; 1984. p. 49-188.
6. Sathvika C, Swathi MS, Mangamoori LN. Phytochemical screening and in vitro antioxidant activity of whole plant extracts of Sesuvium portulacastrum L. Asian J Pharm Clin Res 2018;11:322-7.
7. Miliauskas G, Venskutonis P, Van Beek T. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 2004;85:231-7.
8. Liyana-Pathirana CM, Shahidi F. Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions. J Agric Food Chem 2005;53:2433-40.
9. Madan MP, Raghavan G, Ajay KS, Prabhu P. Free radical scavenging potential of Saussarea costus. Acta Pharm 2005;55:297-304.
10. Hsu CL, Chen W, Weng YM, Tseng CY. Chemical composition, physical properties, and antioxidant activities of yam flours as affected by different drying methods. Food Chem 2003;83:85-92.
11. Stefan M, Gudrun M. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469-74.
12. Clark TE, Appleton CC, Drewes SE. A semi-quantitative approach to the selection of appropriate candidate of plant molluscicides - A South African application. J Ethnopharm 1997;56:1-13.
13. Marston A, Maillard M, Hostettmann K. Search for antifungal, molluscidial and larvicidal compounds from African medicinal plants. J. Ethnopharm 1993;93:215-23.
14. Sinha S, Raghuwanshi R. Phytochemical screening and antioxidant potential of Eclipta prostrata (L) L-A valuable herb. Int J Pharm Pharm Sci 2016;8:255-60.
15. Santhosh S, Velmurugan S, Annadurai R. Phytochemical screening and antimicrobial activity of medicinal plants (Eclipta prostrata L. and Sphaeranthus indicus L.). Int J Pure Appl Biosci 2015;3:271-9.
16. Saxena A, Gautam S, Kamal RA, Rama KS. Comparative study of phytochemicals, antioxidative potential and activity of enzymatic antioxidants of Eclipta alba and Plumbago zyelanica by in vitro assays. Free Radicals Antioxid 2016;6:139-144.
17. Rice-Evans C. Flavonoids and isoflavones: Absorption, metabolism and bioactivity. Free Radical Biol Med 2004;36:827-8.
18. Prior RL, Cao G. Antioxidant phytochemicals in fruits and vegetables: Diet and health implications. Hortic Sci 2000;35:588-92.
19. Pietta P, Simonetti P, Mauri P. Antioxidant activity of selected medicinal plants. J Agric Food Chem 1998;46:4487-90.
20. Rebaya A, Belghith SI, Baghdikian B, Leddet VM, Mabrouki F, Olivier E, et al. Total phenolic, total flavonoid, tannin content, and antioxidant capacity of Halimium halimifolium (Cistaceae). J Appl Pharm Sci 2014;5:52-7.
21. Crozier K, Sinclair M, Kernohan WG, Porter S. Birth technology competence: A concept analysis. Evid Based Midwifery 2006;4:96 100.
22. Blois MS. Antioxidant activity of grape seed extracts on peroxidation models in vitro. J Agri Food Chem 2001;55:1018.
23. Irda F, Kiki RR, Insanu M. In vitro antioxidant activities from various extracts of banana peels using ABTS, DPPH assays and correlation with phenolic, flavonoid, carotenoid content. Int J Pharm Pharm Sci 2014;6:299-303.
24. Parul R, Kundu SK, Saha P. In vitro nitric oxide scavenging activity of methanol extracts of three Bangladeshi medicinal plants. Pharm Innov J 2013;1:83-8.
25. Ialenti A, Moncada S, Di Rosa M. Modulation of adjuvant arthritis by endogeneous nitric oxide. Br J Pharm 1993;110:701-5.
26. Enein AM, El-Baz FK, El-Baroty GS, Youssef AM, El-Baky HH. Antioxidant activity of algal extracts on lipid peroxidation. J Med Sci 2003;3:87-98.
27. Gordon MH. The mechanism of the antioxidant action in vitro. In: Hudson BJ, editor. Food Antioxidant. London: Elsevier; 1990. p. 1-18.
28. Curtis JJ, Mortiz M. Serum enzymes derived from liver cell fraction and response to carbon tetrachloride intoxication in rats. Gastroenterology 1972;62:84-92.
29. Shilpam S, Richa R. Phytochemical screening and antioxidant potential of Eclipta prostrata L-A valuable herb. Int J Pharm Pharm Sci 2016;8:255-60.
Statistics
7 Views | 28 Downloads
How to Cite
M, T., and J. M. “PHYTOCHEMICAL SCREENING AND ANTIOXIDANT ACTIVITY OF ECLIPTA ALBA L.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 215-8, https://innovareacademics.in/journals/index.php/ajpcr/article/view/27828.
Section
Original Article(s)