• ROSHNI RS SONI Department of Applied Sciences, Quest Group of Institutions, Jhanjeri, IKG-Punjab Technical University, SAS Nagar, Punjab, India.
  • MANOJ BALI Baba Hira Singh Bhattal Institute of Engineering and Technology, Lehragaga, IKG-Punjab Technical University, Sangrur, Punjab, India


Objective: The current study aims to study the antioxidant and antimicrobial and antifungal potential of the methanolic extract of Cucurbita pepo var. fastigata seeds (MECS).

Methods: Extraction of the seeds has been carried out with solvents of increasing polarity (chloroform, acetone, and methanol) and the phytochemical study of the methanolic extract have been carried out using standard methods. The free radical scavenging activity of all the extracts was evaluated by DPPH and H2O2 methods. Standard disk diffusion method was used to evaluate antibacterial and antifungal activities.

Results: Phytochemical evaluation showed the maximum presence of triterpenoids, phenolic compounds, tannins and small amount of Coumarins. Methanolic extract revealed momentous antioxidant activity as compared to chloroform and ethyl acetate extract. Hence, methanolic extract of C. pepo. seeds (MECS) at a dose level of 100, 200 and 300 μg/ml was evaluated for antioxidant potential. Maximum free radical scavenging activity of methanolic seed extract of cucurbita pepo var. fastigata has been found at a dose of 300 μg/ml to be 63±0.16 % by 1,1-diphenyl-2-picryl hydrazyl model and at a value of 78% at 300 μg/ml with H2O2 model. Methanolic extract also showed the presence of antibacterial activity.

Conclusion: Presence of phytochemicals in the methanolic extract is responsible for the antioxidant potential. Extracts were investigated for antibacterial activity using the standard disc diffusion assay method against Bacillus subtilis, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa and for the antifungal activities against Aspergillus niger and Candida albicans. The seed extract showed the presence of antibacterial activity, but the antifungal activity was found to be absent in the extract.

Keywords: Antimicrobial, Antioxidant, Cucurbita pepo var. fastigata, Free radical scavenging.


1. Vinale F, Sivasithamparam K, Ghisalberti EL, Woo SL, Nigro M, Marra R, et al. Trichoderma secondary metabolites active on plants and fungal pathogens. Open Mycol J 2014;8:127-39.
2. Sofowora A. African Medicinal Plants. IIe-Ife (Nigeria): University of Ife Press; 1984.
3. Sofowora A. Medicinal Plants and Traditional Medicine in Africa. Ibadan (Nigeria): Spectrum Books Ltd.; 1993.
4. McChesney JD, Venkataraman SK, Henri JT. Plant natural products: Back to the future or into extinction? Phytochemistry 2007;68:2015-22.
5. Newman DJ, Cragg GM. Natural products as sources of new drugs over the last 25 years. J Nat Prod 2007;70:461-77.
6. Newman DJ. Natural products as leads to potential drugs: An old process or the new hope for drug discovery? J Med Chem 2008;51:2589-99.
7. Abid R, Mahmood R, Rajesh KP, Swamy BE. Potential in vitro antioxidant and protective effect of cassia fistula linn: Fruit extracts against induced oxidative damage in human erythrocytes. Int J Pharm Pharm Sci 2014;6:497-505.
8. Dhiman K, Gupta A, Sharma DK, Gill NS, Goyal A. A review on the medicinally important plants of the family Cucurbitaceae. Asian J Clin Nutr 2012;4:16-26.
9. Martha R, Gutierrez P. Review of Cucurbita pepo (Pumpkin) its phytochemistry and pharmacology. Med Chem 2016;6:12-21.
10. Bardaa S, Ben Halima N, Aloui F, Ben Mansour R, Jabeur H, Bouaziz M, et al. Oil from pumpkin (Cucurbita pepo L.) Seeds: Evaluation of its functional properties on wound healing in rats. Lipids Health Dis 2016;15:73.
11. Abdel-Rahman MK. Effect of pumpkin seed (Cucurbita pepo L.) Diets on Benign Prostatic Hyperplasia (BPH ): Chemical and morphometric evaluation in rats. World J Chem 2006;1:33-40.
12. Ratnam N. A review on Cucurbita pepo. Int J Pharmacogn Phytochem Res 2017;9:1190-94.
13. Obi RK, Nwanebu FC, Ndubuisi UU, Orji NM. Antibacterial qualities and phytochemical screening of the oils of Curcubita pepo and Brassica nigra. J Med Plants Res 2009;3:429-32.
14. Jafarian A, Zolfaghari B, Parnianifard M. The effects of methanolic, chloroform, and ethylacetate extracts of the Cucurbita pepo L. On the delay type hypersensitivity and antibody production. Res Pharm Sci 2012;7:217-24.
15. Immaculata M, Insanu M, Anne C, Dass S. Development of immunonutrient from pumpkin (Cucurbita moschata Duchense Ex. Lamk.) Seed. Procedia Chem 2014;13:105-11.
16. Sayed H, Seif A. Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats. Beni Suef Univ J Basic Appl Sci 2014;3:178-85.
17. Medjakovic S, Hobiger S, Ardjomand-Woelkart K, Bucar F, Jungbauer A. Pumpkin seed extract: Cell growth inhibition of hyperplastic and cancer cells, independent of steroid hormone receptors. Fitoterapia 2016;110:150-6.
18. Salman KS, Ashraf S. Reactive oxygen species: A link between chronic inflammation and cancer. Asia Pac J Mol Biol Biotechnol 2013;21:41-9.
19. Kasote DM, Katyare SS, Hegde MV, Bae H. Significance of antioxidant potential of plants and its relevance to therapeutic applications. Int J Biol Sci 2015;11:982-91.
20. Halliwell B. How to characterize an antioxidant: An update. Biochem Soc Symp 1995;61:73-101.
21. Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol 2005;4:685-88.
22. Harborne JB. Phytochemical Methods a Guide to Modern Techniques of Plant Analysis. 3rd ed. Netherlands: Springer; 1998.
23. Takao T, Kitatani F, Watanabe N, Yagi A, Sakata K. A simple screening method for antioxidants and isolation of several antioxidants produced by marine bacteria from fish and shellfish. Biosci Biotechnol Biochem 1994;58:1780-3.
24. Motlhanka DM. Free radical scavenging activity of selected medicinal plants of Eastern Botswana. Pak J Biol Sci 2008;11:805-8.
25. Chang MJ, Ho YL, Huang GJ, Lin IH. Study on the antioxidant activities of crude extracts from the roots of Arnebia euchroma and Lithospermum erythrorhizon. Mid Taiwan J Med 2008;13:113-21.
26. Dutra RC, Leite MN, Barbosa NR. Quantification of phenolic constituents and antioxidant activity of Pterodon emarginatus Vogel seeds. Int J Mol Sci 2008;9:606-14.
27. Stohs SJ, Bagchi D. Oxidative mechanisms in the toxicity of metal ions. Free Radic Biol Med 1995;18:321-36.
28. Walling C. Fenton’s reagent revisite. Acc Chem Res 1975;8:125-31.
29. Siddhuraju P, Manian S. The antioxidant activity and free radical-scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) Seeds. Food Chem 2007;105:950-8.
30. Hochstein P, Atallah AS. The nature of oxidants and antioxidant systems in the inhibition of mutation and cancer. Mutat Res 1988;202:363-75.
31. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
32. Shad AA, Ahmad S, Ullah R, AbdEl-Salam NM, Fouad H, Rehman NU, et al. Phytochemical and biological activities of four wild medicinal plants. Sci World J 2014;2014:857363.
33. Nyakudya E, Jeong JH, Lee NK, Jeong YS. Platycosides from the roots of Platycodon grandiflorum and their health benefits. Prev Nutr Food Sci 2014;19:59-68.
34. Gil MI, Ferreres F, Tomás-Barberán FA. Effect of postharvest storage and processing on the antioxidant constituents (Flavonoids and Vitamin C) of fresh-cut spinach. J Agric Food Chem 1999;47:2213-7.
35. Esmaeili A, Mousavi Z, Shokrollahi M, Shafaghat A. Antioxidant activity and isolation of luteoline from Centaurea behen L. grown in Iran. J Chem 2013;2013:1-5.
36. CLSI. Performance Standards for Antimicrobial Susceptibility Testing. CLSI Supplement M100. Wayne, PA: Clinical and Laboratory Standards Institute; 2017.
37. Singh AR, Bajaj VK, Sekhawat PS, Singh K. Phytochemical estimation and antimicrobial activity of aqueous and methanolic extract of Ocimum sanctum L. J Nat Prod Plant Resour 2013;3:51-8.
38. Mothana RA, Gruenert R, Bednarski PJ, Lindequist U. Evaluation of the in vitro anticancer, antimicrobial and antioxidant activities of some Yemeni plants used in folk medicine. Pharmazie 2009;64:260-8.
39. Siddhuraja P, Becker K. The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata L.) Seed extracts. Food Chem 2007;101:10-9.
40. Gardam MA. Is methicillin-resistant Staphylococcus aureus an emerging community pathogen? A review of the literature. Can J Infect Dis 2000;11:202-11.
41. Lepape A, Monnet DL, participating members, European Society of Intensive Care Medicine. Experience of European intensive care physicians with infections due to antibiotic-resistant bacteria, 2009. Euro Surveill 2009;14:19393.
42. Alviano DS, Alviano CS. Plant extracts: Search for new alternatives to treat microbial diseases. Curr Pharm Biotechnol 2009;10:106-21.
43. Hemaiswarya S, Kruthiventi AK, Doble M. Synergism between natural products and antibiotics against infectious diseases. Phytomedicine 2008;15:639-52.
44. Barnes J, Anderson LA, Phillipson JD. Herbal Meicine. 3rd ed. London: Pharmaceutical Press; 2007.
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How to Cite
RS SONI, R., and M. BALI. “EVALUATION OF ANTIOXIDANT, ANTIMICROBIAL, AND ANTIFUNGAL POTENTIAL OF CUCURBITA PEPO VAR. FASTIGATA SEED EXTRACTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 289-93, doi:10.22159/ajpcr.2019.v12i2.28040.
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