COMBINATORIAL EFFECT OF LEPTIN, TUMOR NECROSIS FACTOR-Î‘LPHA, AND VITAMIN D IN PROGRESSION OF TYPE 2 DIABETES IN KASHMIRI POPULATION

Inshah Din; Sabhiya Majid; Fouzia Rashid; Ishrat Hussain; Rakesh K Koul; Jasiya Qadir; Rabia Farooq

doi:10.22159/ajpcr.2018.v11i10.28097

Authors

Inshah Din Department of Biochemistry, Government Medical College, Srinagar - 190 010, Jammu & Kashmir, India.
Sabhiya Majid Department of Biochemistry, Government Medical College, Srinagar - 190 010, Jammu & Kashmir, India.
Fouzia Rashid Department of Clinical Biochemistry, University of Kashmir, Srinagar - 190 010, Jammu & Kashmir, India.
Ishrat Hussain Department of Medicine, Government Medical College, Srinagar - 190 010, Jammu & Kashmir, India.
Rakesh K Koul Department of Medicine, Government Medical College, Srinagar - 190 010, Jammu & Kashmir, India.
Jasiya Qadir Department of Biochemistry, Government Medical College, Srinagar - 190 010, Jammu & Kashmir, India.
Rabia Farooq Department of Biochemistry, Government Medical College, Srinagar - 190 010, Jammu & Kashmir, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i10.28097

Keywords:

Type 2 Diabetes Mellitus, Leptin, Nil, Vitamin D, Enzyme-linked immunosorbent assay

Abstract

Objectives: Type 2 diabetes mellitus (T2DM) is a metabolic disorder usually characterized by hyperglycemia. Adipose tissue secretes bioactive substances known as adipocytokines including leptin, tumor necrosis factor-alpha (TNF-Î±), and adiponectin. These are considered as a bridge connecting obesity and insulin resistance. There is considerable epidemiological evidence to suggest a role of Vitamin D deficiency in the etiology of T2DM. This study was performed to evaluate and compare serum Vitamin D and adipocytokine levels in T2DM patients and healthy controls of Jammu and Kashmir region of India.

Methods: A total of 200 T2DM patients and normal 200 healthy controls were considered for the study. Enzyme-linked immunosorbent assay was used to measure serum leptin and TNF-Î± levels. The Vitamin D levels were measured by chemiluminescent microparticle immunoassay method. Biochemical parameters were measured by the enzymatic method.

Results: Our results concluded that basic metabolic parameters, TNF-Î± and leptin levels were significantly higher in cases than controls. The serum Vitamin D levels were found significantly lower among T2DM patients. No correlation of Vitamin D with leptin was observed in cases and controls. However, a positive correlation between leptin and TNF-Î± was noted in T2DM subjects as well as in controls with statistical significance being higher in females (r=0.500; p=0.001) as compared to males (r=0.298; p=0.036) in T2DM subjects as well as in controls (r=0.659; p=0.001; r=0.621; p=001).

Conclusion: These results concluded that lowering of Vitamin D levels and upregulation of leptin and TNF-Î± altogether play an important role in the development of T2DM, and from correlation analysis, it could be predicted that leptin and TNF-Î± act in a synergistic manner toward the development of T2DM.

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Author Biography

Inshah Din, Department of Biochemistry, Government Medical College, Srinagar - 190 010, Jammu & Kashmir, India.

Currently the corresponding author of this study is serving as Professor and Head of Biochemistry department of Government medical college Srinagar,
Jammu and Kashmir, India

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