SCREENING OF PHYTOCHEMICALS AND IN VITRO ANTIOXIDANT ANALYSIS OF IXORA MACROTHYRSA (TEJISM. AND BINN.)

  • SURUTHI SARASWATHI G Department of Biochemistry, PSG College of Arts and Science, Coimbatore, Tamil Nadu, India.
  • VICTOR AROKIA DOSS D Department of Biochemistry, PSG College of Arts and Science, Coimbatore, Tamil Nadu, India.

Abstract

Objective: The aim of the study was to investigate the phytochemical screening and quantitative and in vitro antioxidant analysis of the hydroethanolic leaf extract of Ixora macrothyrsa (Tejism. and Binn.)


Methods: The powdered plant material was extracted using different solvents for phytochemical screening. For the quantitative analysis, proteins, carbohydrates, tannins, free amino acids, and proline were estimated. In vitro antioxidants such as ascorbic acid, total phenols, chlorophyll, and carotenoids were also analyzed using standard methods.


Results: The preliminary qualitative analysis of the hydroethanolic leaf extract of I. macrothyrsa showed the presence of carbohydrates, proteins, alkaloids, flavonoids, phenols, saponins, glycosides, and tannins. The secondary metabolites such as proteins, carbohydrates, tannin, free amino acid, and proline were quantified as 24.57±0.61 mg/g, 11.07±0.09 mg/g, 6.17±0.20 mg/g, 9.20±0.17 mg/g, and 34.47±0.44 mg/g, respectively. In vitro antioxidants such as ascorbic acid, total phenols, chlorophyll, and carotenoid were estimated to be 35.73±0.28 mg/g, 15.9±0. mg/g, 9.33±0.09 mg/g, and 5.9±0.58 mg/g, respectively.


Conclusion: It can be concluded that the hydroethanolic extract of the leaves of I. macrothyrsa showed the presence of major secondary metabolites and has potent antioxidant activity.

Keywords: Ixora macrothyrsa, Hydroethanolic leaf extract, Phytochemicals, In vitro antioxidant, Secondary metabolites.

Author Biography

SURUTHI SARASWATHI G, Department of Biochemistry, PSG College of Arts and Science, Coimbatore, Tamil Nadu, India.

Department of Biochemistry, PSG college of Arts & Science, Coimbatore- 641014.

Statistics
41 Views | 84 Downloads
How to Cite
SARASWATHI G, S., and V. A. DOSS D. “SCREENING OF PHYTOCHEMICALS AND IN VITRO ANTIOXIDANT ANALYSIS OF IXORA MACROTHYRSA (TEJISM. AND BINN.)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 128-32, doi:10.22159/ajpcr.2019.v12i2.28106.
Section
Original Article(s)