• DEEPENDRA THAKUR Department of Microbiology, Bangalore University, Bengaluru, Karnataka, India,
  • KAMANA SAHANI Department of Microbiology, Andhra University, Visakhapatnam, Andhra Pradesh, India.


Objective: The objective of the present investigation was to find phytochemical constituents of endophytic fungi Aspergillus sp.3 isolated from Boerhavia diffusa L. stem.

Methods: Both qualitative and quantitative phytochemical analysis was performed.

Results: In the present investigation, endophytic fungi was isolated from the medicinal plant B. diffusa L. The qualitative and quantitative phytochemical analysis was performed from the endophytic fungi Aspergillus sp.3 which showed the presence of phenol, flavonoids, alkaloids, steroids, and terpenoids, 11.66±0.577, 17.66±0.577, 1.746±0.0057, 0.446±0.0057, and 9.746±0.0057 (μg of standard/mg of extract), respectively.

Conclusion: Hence, the endophytic fungi isolated from B. diffusa L. could be regarded as good sources of bioactive compounds for drug formulations.

Keywords: Phytochemical, Phenol, Flavonoids, Alkaloids, Endophytic fungi, Boerhavia diffusa L.


1. Singh MK, Usha S, Singh SC, Singh U. Phytoecological investigations of Boerhavia diffusa L. of Darbhanga district, Bihar. Neo-Botan 1994;2:61-4.
2. Jain SP, Singh SC. Ethno Medical Botanical Survey of Ambikapur District, M.P. Ethnobiology in Human Welfare. Lucknow, Uttar Pradesh, India: Abstracts of the 4th International Congress of Ethnobiology; 1994. p. 293.
3. Rawat AK, Mehrotra S, Tripathi SK, Shome U. Hepatoprotective activity of Boerhavia diffusa L. roots - A popular Indian ethnomedicine. J Ethnopharmacol 2008;56:61-6.
4. Strobel G, Daisy B. Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 2003;67:491-502.
5. Joseph B, Priya M. Bioactive compounds from endophytes and their potential in pharmaceutical effect: A review. Am J Biochem Mol Biol 2011;1:291-309.
6. Bhardwaj R, Yadav A, Sharma R. Phytochemical and antioxidant activity of Boerhavia diffusa. Int J Pharm Pharm Sci 2014;6:344-8.
7. Pooja VA, Lal VK. Punarnava - A natural remedy by ayurveda. Int J Pharm Pharm Sci 2014;6:1-6.
8. Fisher PJ, Petrini O. Tissue specificity by fungi endophytic in Ulex europaeus. Sydow 1987;40:46-50.
9. Suryanarayanan TS, Kumaresan V, Johnson JA. Foliar fungal endophytes from two species of the mangrove Rhizophora. Can J Microbiol 1998;44:1003-6.
10. Schulz B, Wanke U, Drager S, Aust HJ. Endophytes from herbaceous plants and shrubs: Effectiveness of surface sterilization methods. Mycol Res 1993;97:1447-50.
11. Buatong J, Phongpaichit S, Rukachaisirikul V, Sakayaroj J. Antimicrobial activity of crude extracts from mangrove fungal endophytes. World J Microbiol Biotechnol 2011;27:3005-8.
12. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. Pune, India: Nirali Prakashan; 1997.
13. Kokate CK. Practical Pharmacognosy. New Delhi: Vallabh Prakashan; 2005. p. 107-11.
14. Harborne IB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998. p. 302.
15. Manjunath A, Mahadev BG, Shraddha UN. Estimation of total alkaloid in Chitrakadivati by UV-spectrophotometer. Anc Sci Life 2012;31:198-201.
16. Fazel S, Hamidreza M, Rouhollah G, Mohammadreza V. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. Thai J Pharm Sci 2008;32:17-20.
17. Meda A, Lamien CE, Romito M, Millogo J, Nacoulma OG. Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chem 2005;91:571-7.
18. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002;10:178-82.
19. Kim D, Jeong SW, Lee CY. Antioxidant capacity of phenolic phytochemicals from various cultivars of plums. Food Chem 2003;81:321-6.
20. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Viticult 1965;16:144-8.
21. Narayan G, Sondipon C, Shamik G, Samir KS, Suman B. Estimation of Total Terpenoids Concentration in Plant Tissues Using a Monoterpene, Linalool as a Standard Reagent. Protocol Exchange; 2012.
22. Attarde D, Pawar J, Chaudhari B, Pal S. Estimation of sterols content in edible oil and ghee samples. Int J Pharm Sci Rev Res 2010;5:135-7.
23. Hiai S, Oura H, Nakajima T. Color reaction of some sapogenins and saponins with vanillin and sulfuric acid. Planta Med 1976;29:116-22.
24. Polshettiwar SA, Ganjiwale RO, Wadher SJ, Yeole PG. Spectrophotometric estimation of total tannins in some Ayurvedic eye drops. Indian J Pharm Sci 2007;69:574-6.
25. Afify AM, El-Beltagi HS, El-Salam SM, Omran AA. Biochemical changes in phenols, flavonoids, tannins, vitamin E, ?-carotene and antioxidant activity during soaking of three white sorghum varieties. Asian Pac J Trop Biomed 2012;2:203-9.
26. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.
27. Hedge JE, Hofreiter BT. In: whistler RL, Be Miller JN, editor. Carbohydrate Chemistry. 17th ed. New York: Academic Press; 1962.
28. Cook JB, Dumitru AM, O’Buckley TK, Morrow AL. Ethanol administration produces divergent changes in GABAergic neuroactive steroid immune histochemistry in the rat brain. Alcohol Clin Exp Res 2014;38:90-9.
29. Vijay DT, Rajendra SB. Estimation of total phenol, tannin, alkaloids and flavonoids in Hibiscus tiliaceus L. wood extracts. Res Rev J Pharm Phytochem 2014;2:41-7.
30. Pandita N, Vaidya A. Pharmacognostic and phytochemical studies of Cassia absus seed extracts. Int J Pharm Pharm Sci 2016;8:325-32.
13 Views | 25 Downloads
How to Cite
THAKUR, D., and K. SAHANI. “QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL ANALYSIS OF ENDOPHYTIC FUNGI (EF8; ASPERGILLUS SP.3) ISOLATED FROM BOERHAVIA DIFFUSA L., STEM.”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 111-6, https://innovareacademics.in/journals/index.php/ajpcr/article/view/28242.
Original Article(s)