EVALUATION OF PHYTOCONSTITUENTS, AND ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF JUGLANS REGIA FROM KASHMIR REGION
Objectives: The main focus of the present study was to evaluate the antimicrobial efficacy (against oral pathogenic bacteria), free radical scavenging activity, and total phenolic and flavonoids content (TPC and TFC) of methanolic extract (ME) of J. regia obtained from Kashmir region.
Methods: The plant part was collected and its ME was prepared. ME was subjected to antibacterial activity against oral bacteria such as Staphylococcus aureus, Streptococcus mutans, and Pseudomonas aeruginosa. The free radical scavenging activity was determined using 2,2-diphenyl- 1-picrylhydrazylhydrate (DPPH) assay. TPC and TFC were also determined using a standard curve equation of gallic acid and quercetin. A standard curve using different concentrations of gallic acid and quercetin was drawn from which the concentration of phenols in the test sample was calculated and expressed in mg/g.
Results: The ME of J. regia was found effective against all the strains of microorganisms responsible for oral infection understudy. It was also observed that scavenging of DPPH increased with the increase in concentration for both standard ascorbic and methanolic bark extract of J. regia showing its antioxidant potential. The TPC and TFC of ME was found to be 43.35±0.079 and 17.28±0.125.
Conclusions: The results obtained from the study clearly indicate that the walnut bark from Kashmir region can be a good candidate for employment as an antibacterial against oral pathogens. J. regia bark was found to be a good source of healthy compounds such as phenolic and flavonoids, suggesting that its bark could be useful to prevent diseases in which free radicals are present.
2. Silva BM, Andrade PB, Valentão P, Ferreres F, Seabra RM, Ferreira MA, et al. Quince (Cydonia oblonga Miller) fruit (pulp, peel, and seed) and jam: Antioxidant activity. J Agric Food Chem 2004;52:4705-12.
3. Middleton E. Effect of plant flavonoids on immune and inflammatory cell function. In: Flavonoids in the Living System. Boston, MA: Springer; 1998. p. 175-82.
4. Deshpande RR, Kale AA, Ruikar AD, Panvalkar PS, Kulkarni AA, Deshpande NR, et al. Antimicrobial activity of different extracts of Juglans regia L. against oral microflora. Int J Pharm Pharm Sci 2011;3:200-1.
5. Jenkinson HF, Lamont RJ. Oral microbial communities in sickness and in health. Trends Microbiol 2005;13:589-95.
6. Tichy J, Novak J. Extraction, assay, and analysis of antimicrobials from plants with activity against dental pathogens (Streptococcus sp.) J Altern Complement Med 1998;4:39-45.
7. Raj TI, Kumar PU, Rathee R, Dubey K. Screening of some medicinal plants for their antimicrobial activities. Int J Pharm Pharm Sci 2016;8:202-6.
8. Loesche WJ. Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986;50:353-80.
9. Runyoro DK, Ngassapa OD, Matee MI, Joseph CC, Moshi MJ. Medicinal plants used by Tanzanian traditional healers in the management of Candida infections. J Ethnopharmacol 2006;106:158-65.
10. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.
11. Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In: Methods in Enzymol. Vol. 299. San Diego, CA: Academic press; 1999. p. 152-78.
12. Atanassova M, Georgieva S, Ivancheva K. Total phenolic and total flavonoid contents, antioxidant capacity and biological contaminants in medicinal herbs. J Univ Chem Technol Metall 2011;46:81-8.
13. Braca A, Tommasi ND, Bari LD, Pizza C, Politi M, Morelli I. Antioxidant principles from Bauhinia terapotensis. J Nat Prod 2001;64:892-5.
14. Yadav M, Chatterji S, Gupta SK, Watal G. Preliminary phytochemical screening of six medicinal plants used in traditional medicine. Int J Pharm Pharm Sci 2014;6:539.
15. Mohammed NA. Comparison of antimicrobial activities of methanol extracts of Juglans regia against Staphylococcus aureus, and Streptococcus mutans with ciprofloxacin: In Vitro. Almustansiriyah J Sci 2012;23:1-6.
16. Sharma PC, Yelne MB, Dennis TJ, ediotors. “Bhunimba (Andrographics paniculata),” in Data Base on Medicinal Plants used in Ayurveda. Vol. 4. New Delhi, India: Central Council for Research in Ayurveda and Siddha; 2002. p. 34-60.
17. Asha K, Sucheta G, Kavita M, Nirmala D, Jyoti S. Quantification of phenolics and flavonoids by spectrophotometer from-Juglans regia. Inte J Pharma Bio Sci 2010;1:1-4.
18. Ogunmoyole T, Kade IJ, Korodele B. In vitro antioxidant properties of aqueous and ethanolic extracts of walnut (Juglans regia). J Med Plants Res 2011;5:6839-48.
19. Sannigrahi S, Mazumder UK, Pal D, Mishra SL. Hepatoprotective potential of methanol extract of Clerodendrum infortunatum L. against CCl4 induced hepatotoxicity in rats. Indian J Exp Bio 2009;5:394-9.
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