• Ruvanthika Pn Department of Physiology, Tagore Medical College and Hospitals, Chennai - 600127 (PhD program in Bharath University, Chennai, 600073, India) Affiliated to TN Dr. MGR Medical University, Guindy, Chennai - 600 032, Tamil Nadu, India.
  • Manikandan S Department of Physiology, Tagore Medical College and Hospitals, Affiliated to TN Dr. MGR Medical University, Guindy, Chennai - 600 032, Tamil Nadu, India.


Objective: The objective of the study was to evaluate whether ethanolic extracts of Nelumbo nucifera (EENN) seedpod and quercetin (active component of NN) possess antibacterial proprieties against Gram (-) bacteria such as Escherichia coli and Pseudomonas aeruginosa and Gram (+) bacteria such as Staphylococcus aureus.

Methods: Antibacterial activities of EENN seedpod and quercetin were investigated using disc diffusion method, minimum inhibitory concentration against E. coli and P. aeruginosa and Gram (+) bacteria such as S. aureus.

Results: The antibacterial activity of both EENN seedpod and quercetin was found to be increased in dose-dependent manner. The maximum zone of inhibition was exhibited by both EENN seedpod and quercetin against E. coli (14 mm and 15 mm) and P. aeruginosa (13 mm and 15 mm). Gram-negative bacteria were more susceptible to the EENN seedpod extract and quercetin than Gram-positive bacteria.

Conclusion: The results of the present study suggested that the effect of EENN seedpod and quercetin against the tested bacteria in vitro may contribute to the in vivo activities of the EENN seedpod and quercetin.

Keywords: Nelumbo nucifera, Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa.


1. Akinmoladun AC, Ibukun EO, Afor E, Obuotor EM, Farombi EO. Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum. Sci Res Essays 2007;2:163-6.
2. Nascimento GG, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibioticresistant bacteria. Brazil J Microbiol 2000;31:247-56.
3. Nair R, Chanda S. Activity of some medicinal plants against certain pathogenic bacterial strains. Indian J Pharm 2006;38:142-4.
4. Davis PH. Flora of Turkey and East Eagean Island. Edinburg: Edinburg University Press; 1982. p. 47.
5. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
6. Kumaraswamy MV, Kavitha HU, Sathish S. Antibacterial evaluation and phytochemical analysis of Betula utilis against some human pathogenic bacteria. World J Agric Sci 2008;4:661-4.
7. Pitchai D, Manikkam R, Rajendran SR, Pichai G. Database on pharmacophore analysis of active principles from medicinal plants. Bioinformation 2010;5:43-5.
8. Mukherjee PK, Mukherjee D, Maji AK, Rai S, Heinrich M. The sacred lotus (Nelumbo nucifera) - Phytochemical and therapeutic profile. J Pharm Pharm 2009;61:407-22.
9. Vaquero MJ, Alberto MR, De Nadra MC. Antibacterial effect of phenolic compounds from different wines. Food Control 2007;18:93-101.
10. Moharram BA, Al-Mahbashi HM, Saif-Ali R, Ali Aqlan F. Phytochemical, anti-inflammatory, antioxidant, cytotoxic and antibacterial study of Capparis Cartilaginea Decnefrom yemen. Int J Pharm Pharm Sci 2018;10:38-44.
11. Nazemiyeh H, Rahman MM, Gibbons S, Nahar L, Delazar A, Ghahramani MA, et al. Assessment of the antibacterial activity of phenylethanoid glycosides from Phlomis lanceolata against multiple-drug-resistant strains of Staphylococcus aureus. J Nat Med 2008;62:91-5.
12. Shetty SG, Hidayathulla VS, Chandrashekar KR. Antimicrobial activity and phytochemical screening of Pterospermum reticulatum wight and arn. Review article. Int J Pharm Pharm Sci 2011;3:35-7.
13. Boudet AM. Evolution and current status of research in phenolic compounds. Phytochemistry 2007;68:2722-35.
14. Sridhar KR, Rajeev B. Lotus - A potential nutraceutical source. J Agricl Technol 2007;3:143-55.
15. Shen-Miller J, Schopf JW, Harbottle G, Cao RJ, Ouyang S, Zhou KS, et al. Long-living lotus: Germination and soil g-irradiation of centuries-old fruits, and cultivation, growth, and phenotypic abnormalities of offspring. Am J Botany 2002;89:236-47.
16. Duke JA, Bogenschutz-Godwin MJ, du Cellier J, Duke AK. Handbook of Medicinal Herbs. New York: CRC Press; 2002.
17. Mehta NR, Patel EP, Patani PV, Shah B. A review on ethanobotany, phytochemistry and pharmacology. Indian J Pharm Biol Res 2013;1:152-67.
18. Jorgensen JH, Hindler JF. New consensus guidelines from the clinical and laboratory standards institute for antimicrobial susceptibility testing of infrequently isolated or fastidious bacteria. Clin Infect Dis 2006;44:280-6.
19. Zaidan MR, Noor Rain A, Badrul AR, Adlin A, Norazah A, Zakiah I, et al. In vitro screening of five local medicinal plants for antibacterial activity using disc diffusion method. Trop Biomed 2005;22:165-70.1.
20. Sumazian Y, Syahida A, Hakiman M, Maziah M. Antioxidant activities, flavonoids, ascorbic acid and phenolic contents of Malaysian vegetables. J Med Plants Res 2010;4:881-90.
21. Brindha D, Arthi D. Antimicrobial activity of white and pink Nelumbo nucifera gaertn flowers. Asian J Pharm Clin Res Health Care 2010;2:147-55.
22. Burt S. Essential oils: Their antibacterial properties and potential application in foods: A review. Int J Food Microbio 2004;94:223-53.
23. Gill AO, Holley RA. Disruption of Escherichia coli, Listeria monocytogenes and Lactobacillus sakei cellular membranes by plant oil aromatics. Int J Food Microbio 2006;108:1-9.
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How to Cite
Pn, R., and M. S. “IN VITRO ANTIBACTERIAL ACTIVITY OF ETHANOLIC EXTRACT OF SEEDPOD AND QUERCETIN OF NELUMBO NUCIFERA GAERTN”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 164-7, doi:10.22159/ajpcr.2019.v12i1.28915.
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