INSULIN SECRETAGOGUE EFFECT OF ROOTS OF RAVENALA MADAGASCARIENSIS SONN. - AN IN VITRO STUDY

  • SAKTHI PRIYADARSINI S Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.
  • KUMAR PR Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.
  • ABHISHEK ANAND Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.
  • DEVENDIRAN B Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.
  • VENKAT S KADIYAM Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.
  • RICHIE PADMANABH ROY Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

Abstract

Objective: The objective of this study was to establish the cytotoxicity profile and to evaluate the insulin secretagogue effect of ethanolic root extract of Ravenala madagascariensis Sonn.


Methods: The cell viability of rat insulinoma 5F (RIN5F) cell lines over the treatment of plant extract was assessed by 3-(4,5-dimethyl-2-thiazolyl)- 2,5-diphenyltetrazolium bromide assay. The insulin-releasing effect was evaluated by insulin secretion assay over RIN5F cell lines by enzyme-linked immunosorbent assay.


Results: The ethanolic extract of the roots of R. madagascariensis Sonn. showed negligible cytotoxicity at 20–40 μg/ml, and hence, concentrations up to 40 μg/ml were used in insulin secretion assay. The ethanolic root extract at 20 and 40 μg/ml significantly (p<0.05 compared to control) stimulated the insulin release in a dose-dependent manner even in the presence of glucose at lower and higher concentrations (5 and 10 mM).


Conclusion: Thus, our results validate its traditional claim in the treatment of diabetes by stimulating the secretion of insulin, thereby suggesting a possible mechanism of its antidiabetic effect.

Keywords: Insulin secretagogue, Rat insulinoma 5F, Diabetes, In vitro, Ravenala, Insulin secretion assay, Cytotoxicity.

Author Biographies

SAKTHI PRIYADARSINI S, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

ASSISTANT PROFESSOR, 

DEPARTMENT OF PHARMACOGNOSY,

SRM COLLEGE OF PHARMACY,

SRM INSTITUTE OF SCIENCE & TECHNOLOGY,

KATTANKULATHUR-603203.

KUMAR PR, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

PROFESSOR & HEAD, 

DEPARTMENT OF PHARMACOGNOSY,

SRM COLLEGE OF PHARMACY,

SRM INSTITUTE OF SCIENCE & TECHNOLOGY,

KATTANKULATHUR-603203.

ABHISHEK ANAND, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

STUDENT,

SRM COLLEGE OF PHARMACY, 

SRM INSTITUTE OF SCIENCE & TECHNOLOGY,

KATTANKULATHUR, TAMIL NADU, INDIA.

DEVENDIRAN B, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

STUDENT,

SRM COLLEGE OF PHARMACY, 

SRM INSTITUTE OF SCIENCE & TECHNOLOGY,

KATTANKULATHUR, TAMIL NADU, INDIA.

VENKAT S KADIYAM, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

STUDENT,

SRM COLLEGE OF PHARMACY, 

SRM INSTITUTE OF SCIENCE & TECHNOLOGY,

KATTANKULATHUR, TAMIL NADU, INDIA.

RICHIE PADMANABH ROY, Department of Pharmacognosy, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu, India.

STUDENT,

SRM COLLEGE OF PHARMACY, 

SRM INSTITUTE OF SCIENCE & TECHNOLOGY,

KATTANKULATHUR, TAMIL NADU, INDIA.

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PRIYADARSINI S, S., K. PR, A. ANAND, D. B, V. S KADIYAM, and R. PADMANABH ROY. “INSULIN SECRETAGOGUE EFFECT OF ROOTS OF RAVENALA MADAGASCARIENSIS SONN. - AN IN VITRO STUDY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 176-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/29011.
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