CYTO-DEPRESSIVE POTENTIAL OF GANODERMA LUCIDUM, PLEUROTUS OSTREATUS AND THEIR BINARY COMBINATIONS AGAINST MERISTEMATIC CELLS OF ALLIUM CEPA L.
Objective: The current study is designed to evaluate the cyto-depressive effects of Ganoderma lucidum (GL) and Pleurotus ostreatus (PO) and their binary amalgams (GL+PO) on meristematic cells of Allium cepa L.
Methods: Experiments were assessed by utilizing A. cepa bioassay.
Results: Onion knobs were exposed to 500 μg/mL, 1000 μg/mL, and 2000 μg/mL groupings of the concentrates for naturally visible and minute examination. The concentrates essentially repressed the root development of A. cepa L. contrasted with the control in a period of treatment and concentration-dependent manner (p<0.05). In addition, photomicrographs revealed chromosomal aberrations extending from c-mitosis, stickiness, and bridges in the root tip meristematic cells of A. cepa. All mushroom extracts demonstrated remarkable cyto-depressive impacts on meristematic cells of A. cepa L. with some changeability.
Conclusion: In view of the results come out, GL and PO could be considered as potential sources of anticancer mixes. Notwithstanding, additionally thinks about are essential for disengagement and portrayal of the bioactive mixes and more examination on their pharmacological properties is required. It is first report in Bangladesh where we studied cyto-depressive role of edible mushrooms, Ganoderma lucidum (GL) and Pleurotus ostreatus (PO).
2. Islam MR, Omar M, Moyen, Mia R. Phytochemicals and antibacterial activity screening of three edible mushrooms Pleurotus ostreatus, Ganoderma lucidum and Lentinula edodes accessible in Bangladesh. Am J Biol Life Sci 2015;3:31-5.
3. Moyen IS. Antihyperglycemic activity of edible mushroom, Lentinus edodes in alloxan induced diabetic Swiss albino mice. Int J Pharm Clin Res 2014;6:121-6.
4. Tel G, Ozturk M, Duru ME, Turkoglu A. Antioxidant and anticholinesterase activities of five wild mushroom species with total bioactive contents. Pharm Biol Engl 2015;53:824-30.
5. González-Palma I, Escalona-Buendía HB, Ponce-Alquicira E, Téllez-Téllez M, Gupta VK, Díaz-Godínez G, et al. Evaluation of the antioxidant activity of aqueous and methanol extracts of pleurotus ostreatus in different growth stages. Front Microbiol 2016;7:366.
6. Grant WF. Chromosome aberration assays in allium. Mutat Res Genet Toxicol 1982;99:273-91.
7. Fiskesjö G. The allium test as a standard in environmental monitoring. Hereditas 1985;102:99-112.
8. Gutfreund H. Fundamentals of enzyme kinetics. FEBS Lett 1980;116:125.
9. Rojas E, Herrera LA, Sordo M, Gonsebatt ME, Montero R, Rodríguez R, et al. Mitotic index and cell proliferation kinetics for identification of antineoplastic activity. Anticancer Drugs 1993;4:637-40.
10. Sudhakar R, Gowda KNN, Venu G. mitotic abnormalities induced by silk dyeing industry effluents in the cells of Allium cepa. Cytologia (Tokyo) 2001;66:235-9.
11. Çelik TA, Aslantürk Ö. Anti-mitotic and anti-genotoxic effects of Plantago lanceolata aqueous extract on Allium cepa root tip meristem cells. Biologia (Bratisl) 2006;61:62.
12. Schulze E, Kirschner M. Microtubule dynamics in interphase cells. J Cell Biol 1986;102:1020-31.
13. Babich H. The “allium” test: A simple, eukaryote genotoxicity assay. Am Biol Teach 1997;59:580-3.
14. Soliman MI. Genotoxicity testing of neem plant (Azadirachta indica A. Juss.) using the Allium cepa chromosome aberration assay. J Biol Sci 2001;1:1021-7.
15. Bidau CJ, Amat AG, Yajía M, Martí DA, Riglos AG, Silvestroni A. Evaluation of the genotoxicity of aqueous extracts of ilex paraguariensis St. Hil. (Aquifoliaceae) using the allium test. Cytologia (Tokyo) 2004;69:109-17.
16. Chen L, Watkins JF. Evidence against the presence of h2 histocompatibility antigens in ehrlich ascites tumour cells. Nature 1970;225:734.
17. Hoffmann H, Kogler H, Heyse W, Matter H, Caspers M, Schummer D, et al. Discovery, Structure elucidation, and biological characterization of nannocystin, a macrocyclic myxobacterial metabolite with potent antiproliferative properties. Angew Chem Int Ed Engl Ger 2015;54:10145-8.
18. Khatri S, Phougat N, Chaudhary R, Singh B, Chhillar AK. Chemical composition, antioxidant, antibacterial and cytotoxicity analysis of Blumea lacera (burm. F.) Dc. Int J Pharm Pharm Sci 2016;8:313-9.
19. Khajapeer KV, Biswal R, Baskaran R. Evaluation of anti-cml activity of methanol and aqueous extracts of Benkara malabarica (lam.) Tirveng plant leaves. Int J Pharm Pharm Sci 2018;10:20-2.
20. Jeena K, Liju VB, Kuttan R. Antitumor and cytotoxic activity of ginger essential oil (Zingiber officinale Roscoe). Int J Pharm Pharm Sci 2015;7:341-5.
21. Safarzadeh E, Shotorbani SS, Baradaran B. Herbal medicine as inducers of apoptosis in cancer treatment. Adv Pharm Bull 2014;4 Suppl 1:421-7.
22. Lee JM, Kwon H, Jeong H, Lee JW, Lee SY, Baek SJ, et al. Inhibition of lipid peroxidation and oxidative DNA damage by Ganoderma lucidum. Phytother Res Engl 2001;15:245-9.
23. Yang Y, Wang Y, Zhan J, Shi J, Wang X, Liu G, et al. Genetic and cytological analyses of the natural variation of seed number per pod in rapeseed (Brassica napus L.). Front Plant Sci 2017;8:1890.
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