DEVELOPMENT AND CHARACTERIZATION OF BIOCOMPATIBLE POLYHYDROXY BUTYRATE IMPREGNATED WITH HERBAL PLANTS AGAINST WOUND HEALING ACTIVITY ON IN VIVO ANIMAL MODEL

  • RAM NARENDRAN R Research and Development Centre, Bharathiar University, Coimbatore, Tamil Nadu, India.
  • MALEEKA BEGUM SF Department of Biotechnology, Sri Ramakrishna College of Arts and Science, Coimbatore, Tamil Nadu, India.
  • RUBAVATHI S Department of Biotechnology, K.S.R. College of Technology, Tiruchengode, Tamil Nadu, India.

Abstract

Objective: The current study is to evaluate the antimicrobial, antioxidant, anti-inflammatory, and in vitro cytotoxicity activities of polyhydroxybutyrate (PHB) and to develop the herbal impregnated PHB cast film for wound healing activities using Albino Wistar rat model.


Methods: PHB produced by Azotobacter chroococcum A3 strain was synthesized and characterized (previous study). The PHB was subjected to various biocompatibility studies such as antimicrobial, antioxidant, and anti-inflammatory studies. The PHB was also subjected to cytotoxicity study by (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay. PHB films were made using different combinations of plant and algal blends (herbal blends). The herbal blends of PHB films were evaluated for in vivo wound healing activity using Albino Wistar rats.


Results: The turmeric impregnated PHB showed the highest result for antimicrobial with 27.25±0.23 mm against skin pathogens and antioxidant activity with the highest percentage of inhibition of 76%. The result predicts that PHB will not let to any toxic substances rather it acts as a chemoprotective agent followed by the inhibitory concentration value was found to be 1.56 μg/ml for 100 μg. The in vivo study showed better wound healing activity for PHB blended with 2% turmeric leaf and rhizome cast film. Whereas the wound healing activity of control and crude PHB was 90.4±0.4 and 91.3±0.56 respectively.


Conclusion: The results from the present study showed that PHB can act as a good candidate for drug carrier and it is biocompatible in living cells.

Keywords: Polyhydroxybutyrate, Azotobacter chroococcum, Biocompatibility, (3-[4,5-Dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay, Herbal blends, In vivo study.

Author Biographies

RAM NARENDRAN R, Research and Development Centre, Bharathiar University, Coimbatore, Tamil Nadu, India.

Assistant Professor,

School of Biotechnology,

Dr. G.R. Damodaran College of Science,

Coimbatore - 641014

MALEEKA BEGUM SF, Department of Biotechnology, Sri Ramakrishna College of Arts and Science, Coimbatore, Tamil Nadu, India.

Professor and Head,

Department of Biotechnology,

Sri Ramakrishna College of Arts and Science,

Coimbatore - 641006

RUBAVATHI S, Department of Biotechnology, K.S.R. College of Technology, Tiruchengode, Tamil Nadu, India.

Assistant Professor,

Department of Biotechnology,

K.S.R. College of Technology,

Tiruchengode - 637215

References

1. Bonartsev AP, Myshkina VL, Nikolaeva DA, Furina EK, Makhina TA, Livshits VA, et al. Biosynthesis, biodegradation, and application of poly (3- hydroxybutyrate) and its copolymers - Natural polyesters produced by diazotrophic bacteria. Commun Curr Res Edu Topic Trends Appl Microbiol 2007;2007:295-307.
2. Pfeiffer D, Jendrossek D. PhaM is the physiological activator of poly (3- Hydroxybutyrate) (PHB) synthase (PhaC1) in Ralstonia eutropha. Appl Environ Microbiol 2014;80:555-63.
3. Chen GQ, Wu Q. The application of polyhydroxyalkanoates as tissue engineering materials. Biomaterials 2005;26:6565-78.
4. Xavier JR, Babusha ST, George J, Ramana KV. Material properties and antimicrobial activity of polyhydroxybutyrate (PHB) films incorporated with vanillin. Appl Biochem Biotechnol 2015;176:1498-510.
5. Babu PK, Maruthi Y, Pratap S, Sudhakar K, Sadiku R, Prabhakar MN, et al. Development and characterization of polycaprolactone (PCL)/ poly ((R)-3-Hydroxybutyric acid) (PHB) blend microspheres for tamoxifen drug release studies. Int J Pharm Pharm Sci 2015;7:95-100.
6. Gonta S, Savenkova L, Krallish I, Kirilova E. Antimicrobial activity of PHB based polymeric compositions. Environ Eng Manag J 2012;11:S92.
7. Kim MR. Antimicrobial activity of PHB/chitosan films and quality of white bread packaged with the films. Korean J Hum Ecol 2005;14:321 30.
8. Shishatskaya EI, Volova TG. A comparative investigation of biodegradable polyhydroxyalkanoate films as matrices for in vitro cell cultures. J Mat Sci 2004;15:915-23.
9. Wei X, Hu YJ, Xie WP, Lin RL, Chen GQ. Influence of poly (3-hydroxybutyrate-co-4-hydroxybutyrateco-3-hydroxyhexanoate) on growth and osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. J Biomed Mat Res A 2009;90:894-905.
10. Sevastianov VI, Perova NV, Shishatskaya EI, Kalacheva GS, Volova TG. Production of purified polyhydroxyalkanoates (PHAs) for applications in contact with blood. J Biomat Sci Polymer 2003;14:1029-42.
11. Brigham CJ, Sinskey AJ. Applications of polyhydroxyalkanoates in the medical industry. Int J Biotechnol Wellness Indus 2012;1:53-60.
12. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
13. Kirby WM, Bauer AW, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Tech Bullet Regis Med Technol 1966;36:49-52.
14. Mahendran G, Manoj M, Prasad KJ, Bai VN. Antioxidants, anti-proliferative, anti-inflammatory, anti-diabetic and anti-microbial effects of isolated compounds from Swertia corymbosa (Grieb.) Wight ex C.B. Clark – An In vitro approach. Food Sci and Human Wellness 2015;4:169-79.
15. Farahpour MR, Emami P, Ghayour SJ. In vitro antioxidant properties and wound healing activity of hydroethanolic turmeric rhizome extract (ZINGIBERACEAE). Int J Pharm Pharm Sci 2014;6:474-8.
16. Rajeshwaran T, Sumathy R, Kumuthakalavalli R. Wound healing effect of king Alferd’s mushroom (Daldinia concentrica) used by tribes of Sirumalai Hills, Tamil Nadu, India. Int J Pharm Pharm Sci 2017;9:161 4.
17. Pachekoski WM, Agnelli JAM, Belem LP. Thermal, mechanical and morphological properties of poly (Hydroxybutyrate) and polypropylene blends after processing. Mat Res 2009;12:159-64.
18. Monshupanee T, Nimdach P, Incharoensakdi A. Two-stage (photoautotrophy and heterotrophy) cultivation enables efficient production of bioplastic poly-3-hydroxybutyrate in auto-sedimenting cyanobacterium. Sci Rep 2016;6:37121.
19. Gayathri A, Sekar DS, Sakthi R. Wound healing activity of Curcuma longa with Oleum olivae. J Aca Ind Res 2015;3:479-80.
20. Simon-Colin C, Gueguen Y, Bachere E, Kouzayha A, Saulnier D, Gayet N, et al. Use of natural antimicrobial peptides and bacterial biopolymers for cultured pearl production. Mar Drugs 2015;13:3732 44.
21. Mogo?anu GD, Grumezescu AM. Natural and synthetic polymers for wounds and burns dressing. Int J Pharm 2014;463:127-36.
22. Kundu S, Biswas TK, Das P, Kumar S, De DK. Turmeric (Curcuma longa) rhizome paste and honey show similar wound healing potential: A preclinical study in rabbits. Int J Low Extrem Wounds 2005;4:205 13.
23. Narendhirakannan RT, Nirmala JG, Caroline A, Lincy S, Saj M, Durai D. Evaluation of antibacterial, antioxidant and wound healing properties of seven traditional medicinal plants from India in experimental animals. Asian Pac J Trop Bio 2012;2012:S1245-53.
24. Karri VV, Kuppusamy G, Talluri SV, Mannemala SS, Kollipara R, Wadhwani AD, et al. Curcumin loaded chitosan nanoparticles impregnated into collagen-alginate scaffolds for diabetic wound healing. Int J Biol Macromol 2016;93:1519-29.
25. Nguyen VC, Nguyen VB, Hsieh MF. Curcumin-loaded chitosan/gelatin composite sponge for wound healing application. Int J Poly Sci 2013. Doi.org/10.1155/2013/106570.
26. Dai X, Liu J, Zheng H, Wichmann J, Hopfner U, Sudhop S, et al. Nano-formulated curcumin accelerates acute wound healing through Dkk-1-mediated fibroblast mobilization and MCP-1-mediated anti-inflammation. NPG Asia Mat 2017;9:1-14.
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How to Cite
R, R. N., M. BEGUM SF, and R. S. “DEVELOPMENT AND CHARACTERIZATION OF BIOCOMPATIBLE POLYHYDROXY BUTYRATE IMPREGNATED WITH HERBAL PLANTS AGAINST WOUND HEALING ACTIVITY ON IN VIVO ANIMAL MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 196-01, https://innovareacademics.in/journals/index.php/ajpcr/article/view/29179.
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