• YADAV AV Department of Pharmacology, MCE Society’s Allana College of Pharmacy, Pune, Maharashtra, India.
  • UNDALE VR Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pune, Maharashtra, India.


Objective: Bhasmas are biologically produced nanoparticles prescribed with several other medicines of Ayurveda. Metal-based drugs are prepared by transmutation of base metals into noble ones along with the use of plant extracts meant to eradicate the toxic effects of metal. Naga bhasma (NB) is one of such metallic preparation used in various diseases such as diarrhea, spleen enlargement, and diabetes. The present study aims to test the hematinic activity of NB against phenylhydrazine (PHZ)-induced anemia in rats.

Methods: The experiment was carried on Wistar rats of either sex (150–200 g). Anemia was induced by an oral administration of PHZ 40 mg/kg for a period of 2 days. Hemoglobin (Hb) was measured on the 3rd day. Rats with Hb <11 g/dl were selected for the study. The animals were divided into six groups containing six animals each. Group I served as normal control, Group II as anemic control, and Group III was considered as standard that received ferrous sulfate (100 mg/kg), while Groups IV, V, and VI were treated with test NB 100, 200, and 400 mg/kg, respectively, for 15 days. Hb was checked on the 0, 2nd, 7th, and 15th days.

Results: Oral administration of PHZ decreased Hb from 14 g/dl at day 0 to 11 g/dl at day 2. NB induced a significant increase in Hb concentration to 13 at the dose of 100 mg/kg p.o. after 48 h.

Conclusion: PHZ decreased Hb rate inducing anemia. NB caused an increase in the Hb values in anemic rats. Thus, it can be concluded that NB exhibits significant hematinic activity in rats.

Keywords: Naga bhasma, Anemia, Phenylhydrazine.


1. Cappellini MD, Motta I. Anemia in clinical practice-definition and classification: Does hemoglobin change with aging? Semin Hematol 2015;52:261-9.
2. Idu MD, Igbafe G, Erhabor J. Anti-anaemic activity of jatropha tanjorensis ellis and saroja in rabbits. J Med Plants Stud 2014;2:64-72.
3. Pingali PS, Srinivas P, Madhava RB. Study of anti-anaemic effect of Schrebera swietenioides roxb. In rat models. Asian J Pharm Clin Res 2015;8:260-3.
4. Ankur J, Soni P, Vyas N, Khan J, Malviya S, Kharia A. Anti-anemic activity of hydro-alcoholic leaf extract of Aegle marmelos in phenylhydrazine induced anemic rats. Int J Curr Res 2017;9:48928-31.
5. Singh SK, Gautam DN, Kumar M, Rai SB. Synthesis, characterization and histopathological study of a lead-based Indian traditional drug: Naga bhasma. Indian J Pharm Sci 2010;72:24-30.
6. Nagarajan S, Pemiah B, Krishnan UM, Rajan KS, Krishnaswamy S, Swaminathan S. Physicochemical characterization of lead based Indian traditional medicine - Naga bhasma. Int J Pharm Pharm Sci 2012;4:69-74.
7. Deshmukh SM, Bhingare CL, Kshirsagar SJ. Screening of antidiabetic effect of naga bhasma in alloxan induced hyperglycemic rats. Int J Res Ayurveda Pharm 2013;4:240-3.
8. Subedi RP, Vartak RR, Kale PG. Study of general properties of abhrak bhasma: A nanomedicine. Int J Pharm Sci Rev Res 2017;44:238-42.
9. Roque M, Anna C, Gatti C, Veuthey T. Haematological and Morphological analysis of erythropoietic regenerative responses in phenylhydrazine induced hemolytic anemia in mice. Scand J Lab Anim Sci 2008;35:181-90.
10. Joshi A, Gupta D, Soni P, Malviya S, Jhade D, Kharia A. Anti-anemic activity of hydroalcoholic leaf extract of Tamarindus indica in phenylhydrazine induced anemic rats. J Harmoniz Res Appl Sci 2017;5:132-5.
11. Baskaran K, Suruthi B. Anti-anemic activity of ethanolic leaf extract of Kedrostis foetidissima in phenylhydrazine induced anemic rats. Sch Acad J Biosci 2016;4:681-3.
12. Berger J. Phenylhydrazine haematotoxicity. J Appl Biomed 2007;5:125-30.
13. Pal D, Sahu CK, Haldar A. Bhasma: The ancient Indian nanomedicine. J Adv Pharm Technol Res 2014;5:4-12.
14. Sarkar PK, Prajapati PK, Shukla VJ, Ravishankar B, Choudhary AK. Toxicity and recovery studies of two ayurvedic preparations of iron. Indian J Exp Biol 2009;47:987-92.
15. Singh TR, Gupta LN, Kumar N, Kumar V. Anti-diabetic activity of shilajatvadi lauha, an ayurvedic traditional herbomineral formulation. Int J Health Allied Sci 2016;5:9-14.
16. Hussain A, Joshi A, Malviya S, Kharia A. Anti-anemic activity of seeds of Trigonella Foenum-graecum in male albino rats. J Pharm Biomed Sci 2016;6:608-12.
17. Shukla S, Mehta A, John J, Singh S, Mehta P, Vyas S. Clinical manifestations of human parvovirus B19 in adults. Arch Int Med 2012;149:11532-6.
6 Views | 20 Downloads
How to Cite
AV, Y., and U. VR. “EVALUATION OF HEMATINIC ACTIVITY OF NAGA BHASMA IN PHENYLHYDRAZINE-INDUCED ANEMIA IN EXPERIMENTAL RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 202-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/29256.
Original Article(s)