NARINGENIN-LOADED D-Α-TOCOPHERYL POLYETHYLENE GLYCOL SUCCINATE 1000 POLYMERIC NANOSUSPENSION: AN IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITY

  • SUMATHI RAJAMANI Nandha College of Pharmacy and Research Institute, Erode - 52, Tamil Nadu, India.
  • GOBINATH KALYANA SUNDARAM Nandha College of Pharmacy and Research Institute, Erode - 52, Tamil Nadu, India.
  • TAMIZHARASI SENGODAN Nandha College of Pharmacy and Research Institute, Erode - 52, Tamil Nadu, India.
  • SIVAKUMAR THANGAVELU Nandha College of Pharmacy and Research Institute, Erode - 52, Tamil Nadu, India.
  • NIKHITHA K SHANMUKHAN Department of Pharmaceutics, JSS College of Pharmacy, Ootacamund, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.
  • ARUN RADHAKRISHNAN Department of Pharmaceutics, JSS College of Pharmacy, Ootacamund, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

Abstract

Objective: Naringenin (NAR) a flavonoid, exhibits extensive pharmacological action, fails to attain a significance in application due to low aqueous solubility (~ 0.214 mg/mL) which results in low bioavailability (5.8%). Nanosuspension of NAR (NARNS) was prepared in our previous studies using high-pressure homogenization employing various polymers. All these formulations were characterized and as a continuation of our work formulations was further evaluated for their anti-inflammatory activity by in vitro and in vivo methods.


Methods: Denaturation of protein method and membrane stabilization methods was chosen for in vitro evaluation. In vivo studies performed were acute inflammatory studies (carrageenan-induced paw edema) and chronic inflammatory studies (cotton pellet granuloma) on Wistar albino rats.


Results: The studies demonstrated that the NAR and NARNS at a dose of 50mg/kg P.O. have a potent activity compared to the standard drug diclofenac.


Conclusion: The percentage of protection against inflammation exhibited by NARNS was highly significant compared to NAR.

Keywords: Carrageenan, Oxidative stress, Anti-inflammatory, Naringenin.

Author Biography

ARUN RADHAKRISHNAN, Department of Pharmaceutics, JSS College of Pharmacy, Ootacamund, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

Pharmaceutics

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RAJAMANI, S., G. KALYANA SUNDARAM, T. SENGODAN, S. THANGAVELU, N. K. SHANMUKHAN, and A. RADHAKRISHNAN. “NARINGENIN-LOADED D-Α-TOCOPHERYL POLYETHYLENE GLYCOL SUCCINATE 1000 POLYMERIC NANOSUSPENSION: AN IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 459-463 Article Retracted, https://innovareacademics.in/journals/index.php/ajpcr/article/view/29331.
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