FORMULATION AND EVALUATION OF ANTIMICROBIAL OINTMENTS FROM EUPATORIUM GLANDULOSUM HORT. EX KUNTH

  • SILPA M Department of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.
  • SURESH JOGHEE Department of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.
  • HAMSALAKSHMI Department of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

Abstract

Objective: The present study was undertaken to evaluate the antimicrobial activity of an ointment prepared from alcohol, ethyl acetate, and chloroform extracts of Eupatorium glandulosum Hort. ex Kunth.


Methods: The dried plant materials of E. glandulosum were individually extracted using alcohol, ethyl acetate, and chloroform by Soxhlet method. These three different types of extracts were used for the preparation of ointment and evaluated for the phytochemicals, pH, homogeneity, spreadability, tube extrudability, stability, and antimicrobial activity. The activity of prepared ointments was evaluated against Escherichia coli and Bacillus subtilis and the in vitro antimicrobial activity of ointments of plant extracts was performed by cup plate methods. The most effective antimicrobial ointment was determined by comparing the results of the zone of inhibition of all the ointments of each solvent extract.


Results: The antimicrobial activity of optimized formulation was showed significant activity against the tested bacterial pathogens.


Conclusion: The present study confirmed the antimicrobial activity of the formulations. Furthermore, it can use as a potential dosage form for clinical utility. The results are the justification for the use of the plant in folk medicine.




 

Keywords: Eupatorium glandulosum, Herbal ointment, Cup plate method, Stability

Author Biography

SILPA M, Department of Pharmacognosy, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

Department of pharmacognosy

References

1. Liu S, Long Q, Xu Y, Wang J, Xu Z, Wang L, et al. Assessment of antimicrobial and wound healing effects of brevinin-2Ta against the bacterium Klebsiella pneumoniae in dermally-wounded rats. Oncotarget 2017;8:111369-85.
2. Shihabudeen HM, Priscilla DH, Thirumurugan K. Antimicrobial activity and phytochemical analysis of selected Indian folk medicinal plants. Int J Pharma Sci Res 2010;1:430-4.
3. Alavijeh PK, Alavijeh PK, Sharma D. A study of antimicrobial activity of few medicinal herbs. Asian J Plant Sci Res 2011;2:496-502.
4. Makinde AA, Igoli JO, TA’Ama L, Shaibu SJ, Garba A. Antimicrobial activity of Cassia alata. Afr J Biotechnol 2007;6:1509-10.
5. Parekha J, Chanda SV. Antibacterial activity of aqueous and alcoholic extracts of 34 Indian medicinal plants against some Staphylococcus species. Turk J Biol 2008;32:63-71.
6. Stary F, Hans S. The National Guides to Medical Herbs and Plants. UK: Tiger Books International; 1998.
7. Gislene GF, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Braz J Microbiol 2000;31:247-56.
8. Ashok GK. Introduction to Pharmaceutics-1, New Syllabus Implemented in the Year 1993, According to Regulation 1991. 3rd ed. Chennai: CBS Publishers; 2006. p. 13.
9. Gaud RS, Dr. Yeole PG, Yadav AV, Gokhale SB. Textbook of Pharmaceutics. 10th ed. Pune: Nirali Prakashan; 2008. p. 8.
10. Usha YS, Ashish AM. Review on: An ointment. Int J Pharmacogn Phytochem Res 2015;4:170-92.
11. Desingh M, Jesudas JM, Balasubramaniam P, Mayakrishnan P, Ganesan B, Mohan R. Phytochemical analysis and antimicrobial activity of Eupatorium glandulosum. Int J Curr Microbiol App Sci 2014;3:882-5.
12. Iyeswarya MB, Manigundan K, Usha J, Devi PS, Priya AS. Phytochemical study of methanolic leaf extract of Eupatorium glandulosum L. BioMedRx 2013;1:406-9.
13. Sasikumar JM, Doss AP, Doss A. Antibacterial activity of Eupatorium glandulosum leaves. Fitoterapia 2005;76:240-3.
14. Altoun ME. Altoun’s Pharmaceutics the Design and Manufacture of Medicines. 3rd ed. London: Elsevier, Churchill Livingstone; 2007. p. 593.
15. Chhetri HP, Yogol NS, Sherchan J, Anupa KC, Mansoor S, Panna Thapa Kathmandu. Uni J Sci 2010;6:102-7.
16. Chhetri HP, Yogol NS, Sherchan J, Anupa KC, Mansoor S, Thapa P. Formulation and evaluation of antimicrobial herbal ointment. Kathmandu Univ J Sci Eng Tech 2010;6:102-7.
17. Ravindra TJ, Pratibha RP, Payal HP. Formulation and evaluation of semisolid preparation (ointment, gel and cream) of thiocolchicoside. J Pharm Biomed Sci 2011;8:1-6.
18. Dey S, Mazuder B, Patel JR. Enhance percutaneous permeability of acyclovir by DMSO from topical gel formulation. Int J Pharm Sci Drug Res 2009;2:384-91.
19. Ketkomol P, Punjanon T. Comparative studies on physicochemical properties of Morinda citrifolia Gel and ointment formulations. Asian J Pharm Clin Res 2019;12:208-12.
20. Akanksha D, Vikas G, Neetesh KJ, Shailendra S, Neelam B, Dinesh KJ. Formulation and evaluation of neomycin sulphate ointment containing natural wound healing agent curcuma longa. Int J Pharm Sci Drug Res 2009;1:116-8.
21. Rawat S, Singh R, Thakur P, Kaur S, Semwal A. Wound healing agents from medicinal plants: A review. Asian Pac J Trop Biomed 2012;2:1910-7.
22. Jyothi D, Koland M, Priya S. Investigation of anti-inflammatory activity of ointments containing fenugreek extract. Asian J Pharm Clin Res 2014;7:66-9.
23. Panda P, Ghosh A. Formulation and evaluation of topical dosage form of Eupatorium odoratum Linn. And their wound healing activity. Int J Pharma Bio Sci 2010;1:201-3.
24. Mohanta GP, Jamal M, Umdevi S. Formulation and evaluation of a polyherbal wound healing cream. Indian Drugs 2007;44:281-81.
25. Sumathi S, Banupriya SJ, Akhila V, Padma PR. Characterization, antimicrobial, and metabolic activity of green and chemically synthesized zinc oxide nanoparticles. Asian J Pharm Clin Res 2019;12:11-7.
26. Shinde AJ, Bhise SB, Jarag RJ, Jadhav NR. Preparation of cream containing Tridax procumbens, Curcuma longa and Azadirachta indica and its evaluation for wound healing property. Indian Pharm 2005;4:107-10.
27. Kamble P, Wadher S. Synthesis, in vitro antioxidant and antimicrobial evaluation of 3-hydroxy chromone derivatives. Asian J Pharm Clin Res 2018;1:259-68.
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SILPA M, SURESH JOGHEE, and HAMSALAKSHMI. “FORMULATION AND EVALUATION OF ANTIMICROBIAL OINTMENTS FROM EUPATORIUM GLANDULOSUM HORT. EX KUNTH”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 1, Dec. 2019, pp. 224-8, doi:10.22159/ajpcr.2020.v13i1.29533.
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