EVALUATION OF ANTICANCER POTENTIAL OF ETHANOLIC EXTRACT OF ALANGIUM SALVIFOLIUM LEAVES IN ANIMAL MODELS

  • PRATHIMA C Department of Pharmacology, JSS Medical College, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India. http://orcid.org/0000-0003-4261-883X
  • SHASHIKUMARA SHASHIKUMARA Department of Pharmacology, Chamarajanagar Institute of Medical College, Yadapura, Karnataka, India.
  • THIPPESWAMY T Department of General Medicine, JSS Medical College, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

Abstract

Objective: The present study aims to evaluate the anticancer potential of ethanolic extract of Alangium salvifolium leaves (EASL) against Ehrlich ascites carcinoma (EAC) in animal model.


Methods: The ethanolic extract of A. salvifolium was prepared by continuous method using Soxhlet apparatus. Phytochemical analysis of EASL was conducted. Anticancer activity of the extract was evaluated by EAC model in Swiss albino mice. EASL was administered at the doses of 100 and 200 mg/kg body weight once a day orally for 14 days, after 24 h of tumor inoculation.


Results: A. salvifolium leaves extract treatment produced significant decrease in tumor volume and body weight, and increase in total and non-viable cell count, along with elevated life span of EAC-bearing mice. The EASL exhibited potent dose-dependent antitumor activity. Further, the altered hematological parameters such as red blood cells, hemoglobin, and white blood cells levels as well as biochemical parameters such as serum glutamic oxaloacetic transaminase, serum glutamate pyruvate transaminase, alkaline phosphatase, albumin, and total protein levels in EAC mice were significantly (p<0.05) regained following treatment with EASL. The anticancer potential of EASL can be attributed to the presence of phenols and flavonoids.


Conclusion: Results suggest that EASL possesses significant anticancer activity against EAC tumor-bearing Swiss albino mice.

Keywords: Anticancer, Alangium salvifolium, Ehrlich ascites carcinoma.

Author Biography

PRATHIMA C, Department of Pharmacology, JSS Medical College, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

Assistant Professor

Department of Pharmacology

References

1. Dashora N, Sodde V, Bhagat J, Prabhu KS, Lobo R. Antitumor activity of Dendrophthoe falcate against Ehrlich ascites carcinoma in Swiss albino mice. Pharm Crops 2010;2:1-7.
2. American Cancer Society. Global Cancer Facts and Figures 2007. Atlanta, GA: American Cancer Society; 2007.
3. Lyon B. In: Levin BE, editor. World Cancer Report 2008. Geneva: IARC Press; 2008.
4. Yin SY, Wei WC, Jian FY, Yang NS. Therapeutic applications of herbal medicines for cancer patients. Evid Based Complement Alternat Med eCAM 2013;2013:302426.
5. Pearce A, Haas M, Viney R, Pearson SA, Haywood P, Brown C, et al. Incidence and severity of self-reported chemotherapy side effects in routine care: A prospective cohort study. PLoS One 2017;12:e0184360.
6. Anantharaju PG, Gowda PC, Vimalambike MG, Madhunapantula SV. An overview on the role of dietary phenolics for the treatment of cancers. Nutr J 2016;15:99.
7. Venkateshwarlu R, Raju AB, Yerragunta VG. Phytochemistry and pharmacology of Alangium salvifolium: A review. J Pharm Res 2011;4:1423-5.
8. Nahar L, Zahan R, Mosaddik A, Islam S, Haque A, Fazal A, et al. Antioxidant and antitumor activity of chloroform extract of Alangium salvifolium flowers. Phytopharmacology 2012;2:123-34.
9. Ravikumar AR, Madgaonkar V, Venkatesha RT, Bharathi R, Murthy KV. Potential cytotoxic drug effects of secondary metabolites derived from selected medicinal plants of Savanadurga forest in Karnataka. Int J Pharm Pharm Sci 2014;6:238-41.
10. Parameshwar P, Reddy YN. Protective role in-vitro free radical scavenging activities of Alangium salvifolium (linn) againest CCL4 induced hepatic damage in rats. Int J Pharm Pharm Sci 2015;7:447-52.
11. Srikanth P, Sudhakara K, Basha GG, Muruli K, kumar SA. Antiulcer effect of Alangium salvifolium ethanolic leaf extract on gastric lesion induced by ethanol rats. Asian J Pharm Clin Res 2011;4:112-4.
12. Kuttan G, Vasudevan DM, Kuttan R. Isolation and identification of a tumor reducing component from mistletoe extract (Iscador). Cancer Lett 1988;41:307-15.
13. Gupta M, Mazumder UK, Rath N, Mukhopadhyay DK. Antitumor activity of methanol extract of Cassia fistula L. Seed against Ehrlich ascites carcinoma. J Ethnopharmacol 2000;72:151-6.
14. Shashikumara S, Prathima C, Sibgatullah M. Evaluation of antidepressant activity of ethanolic extract of Alangium salvifolium leaves in Swiss albino mice. Biomed Pharm J 2017;10:427-33.
15. Fotsis T, Pepper MS, Aktas E, Breit S, Rasku S, Adlercreutz H, et al. Flavonoids, dietary-derived inhibitors of cell proliferation and in vitro angiogenesis. Cancer Res 1997;57:2916-21.
16. Viral D, Shivanand P, Jivani N. Anticancer evaluation of Adiantum venustum Don. J Young Pharm 2011;3:48-54.
17. Marappan S, Subramaniyan A. Antitumor activity of methanolic extract of Cynodon dactylon leaves against Ehrlich ascites induced carcinoma in mice. J Adv Sci Res 2012;3:105-8.
Statistics
21 Views | 47 Downloads
How to Cite
C, P., S. SHASHIKUMARA, and T. T. “EVALUATION OF ANTICANCER POTENTIAL OF ETHANOLIC EXTRACT OF ALANGIUM SALVIFOLIUM LEAVES IN ANIMAL MODELS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 397-00, https://innovareacademics.in/journals/index.php/ajpcr/article/view/29601.
Section
Original Article(s)