BIO-CONTROL OF MULTIPLE DRUG-RESISTANT UROPATHOGENS USING MEDICINAL PLANT EXTRACTS
Objective: The present study was conducted to evaluate the potential of some medicinal plants used in Ayurveda in treating multiple drug-resistant human pathogens causing urinary tract infections (UTIs).
Methods: Dried parts of six medicinal plants used in Ayurveda for treating UTI were Soxhlet extracted, and the extract was concentrated in vacuo. Various concentrations of the extract were tested for antimicrobial activity against three clinical isolates of multiple drug-resistant bacteria causing UTI.
Results: Preliminary results showed the promising antibacterial effect of plant extracts. Escherichia coli, the most common pathogen associated with UTI, was susceptible to aqueous extracts of all the six medicinal plants.
Conclusion: This study concluded that the medicinal plants used in Ayurveda to treat UTIs are effective against multiple drug-resistant uropathogens. Further study in this regard may lead to the identification of novel antimicrobial agent for treating multiple drug-resistant urinary tract pathogens.
2. Chaudhary V, Sharma G, Chaudhary N, Raghuvanshi RK. High prevalence of multiple drug resistance among pediatric Escherichia coli infections. Int J Med Res Health Sci 2016;5:166-9.
3. Cohen ML. Epidemiology of drug resistance: Implications for a post - Antimicrobial era. Science 1992;257:1050-5.
4. World Health Organization. Antimicrobial Resistance: Global Report on Surveillance. Geneva: World Health Organization; 2014.
5. Scalbert A. Antimicrobial properties of tannins. Phytochemistry 1991;30:3875-83.
6. Eloff JN. Which extractant should be used for the screening and isolation of antimicrobial components from plants? J Ethnopharmacol 1998;60:1-8.
7. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
8. Erdogrul ÖT. Antibacterial activities of some plant extracts used in folk medicine. Pharm Biol 2002;40:269-73.
9. Harborne JB. Phenolic compounds. In Phytochemical Methods. Dordrecht: Springer; 1984. p. 37-99.
10. Wagner H, Blandt S, Zgainski EM. Plant Drug Analysis. New York: Spring-Verlag; 1984. p. 320.
11. Satyanand TR, Amol PP, Ajeet S, Ashok B, Patel BD. Antibacterial activity of aqueous and ethanolic extracts of Scindapsus officinalis (Roxb.) Schott. Adv Biol Res 2011;5:77-80.
12. Nascimento GG, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazil J Microbiol 2000;31:247-56.
13. Smania A Jr., Monache FD, Smania ED, Cuneo RS. Antibacterial activity of steroidal compounds isolated from Ganoderma applanatum (Pers.) Pat. (Aphyllophoromycetideae) fruit body. Int J Med Mushr 1999;1:325-30.
14. Singh B, Sahu PM, Sharma MK. Anti-inflammatory and antimicrobial activities of triterpenoids from Strobilanthes callosus Nees. Phytomedicine 2002;9:355-9.
15. Dasgupta N, Ranjan S, Saha P, Jain R, Malhotra S, Saleh MA. Antibacterial activity of leaf extract of Mexican marigold (Tagetes erecta) against different Gram-positive and Gram-negative bacterial strains. J Pharm Res 2012;5:4201-3.
16. Basri DF, Fan SH. The potential of aqueous and acetone extracts of galls of Quercus infectoria as antibacterial agents. Indian J Pharm 2005;37:26.
17. Thangavel A, Balakrishnan S, Arumugam A, Duraisamy S, Muthusamy S. Phytochemical screening, gas chromatography-mass spectrometry (GC-MS) analysis of phytochemical constituents and anti-bacterial activity of Coraindrum sativum (L.) seeds. Int J Pharm Pharm Sci 2015;7:153-9.
18. Bonjar GS. Screening for antibacterial properties of some Iranian plants against two strains of Escherichia coli. Asian J Plant Sci 2004;3:310-4.
19. Bonjar S. Evaluation of antibacterial properties of some medicinal plants used in Iran. J Ethnopharmacol 2004;94:301-5.
20. Elgayyar M, Draughon FA, Golden DA, Mount JR. Antimicrobial activity of essential oils from plants against selected pathogenic and saprophytic microorganisms. J Food Prot 2001;64:1019-24.
21. Tolkunova NN. Effect of essential oils on microbiologic indices of meat products. In: Agropromizdat. Moskva: Pishchevaia Promyshlennost; 2002. p. 56-9.
22. Delaquis PJ, Stanich K, Girard B, Mazza G. Antimicrobial activity of individual and mixed fractions of dill, cilantro, coriander and eucalyptus essential oils. Int J Food Microbiol 2002;74:101-9.
23. Lo Cantore P, Iacobellis NS, De Marco A, Capasso F, Senatore F. Antibacterial activity of Coriandrum sativum L. And Foeniculum vulgare miller var. Vulgare (Miller) essential oils. J Agric Food Chem 2004;52:7862-6.
24. Matasyoh JC, Maiyo ZC, Ngure RM, Chepkorir R. Chemical composition and antimicrobial activity of the essential oil of Coriandrum sativum. Food Chem 2009;113:526-9.
25. Kubo I, Fujita K, Kubo A, Nihei K, Ogura T. Antibacterial activity of coriander volatile compounds against Salmonella choleraesuis. J Agric Food Chem 2004;52:3329-32.
26. Rattanachaikunsopon P, Phumkhachorn P. Potential of coriander (Coriandrum sativum) oil as a natural antimicrobial compound in controlling Campylobacter jejuni in raw meat. Biosci Biotechnol Biochem 2010;74:31-5.
27. Saeed S, Tariq P. Antimicrobial activities of Emblica officinalis and Coriandrum sativum against gram positive bacteria and Candida albicans. Pak J Bot 2007;39:913-7.
28. Saeed S, Tariq P. Antibacterial activities of Emblica officinalis and Coriandrum sativum against gram negative urinary pathogens. Pak J Pharm Sci 2007;20:32-5.
29. A?ao?lu S, Dostbil N, Alemdar S. Antimicrobial effect of seed extract of cardamom (Elettar?a cardamomum Maton). YÜ Vet Fak Derg 2006;16:99-101.
30. Aneja KR, Sharma C. Atimicrobial potetial of fruit extracts of Elettaria cardamomum Mato (chhoti elaichi) against the pathoges causing ear infection. Pharmacologyonline 2010;3:750-6.
31. Sawhney SS, Painuli RM, Chauhan NE. Evaluation of bactericidal and anticancer properties of fruits of Piper longum. Int J Pharm Pharm Sci 2011;3:282-7.
32. Reddy PS, Jamil K, Madhusudhan P, Anjani G, Das B. Antibacterial activity of isolates from Piper longum and Taxus baccata. Pharm Biol 2001;39:236-8.
33. Khan M, Siddiqui M. Antimicrobial activity of Piper fruits. Nat Prod Radiance 2007;6:111-3.
34. Ali MA, Alam NM, Yeasmin MS, Khan AM, Sayeed MA, Rao VB. Antimicrobial screening of different extracts of Piper longum Linn. Res J Agri Biol Sci 2007;3:852-7.
35. Prashanti P, Anita S, Shashidhara S. Studies on the antibacterial activity of the aqueous extract of the roots of Rotula aquatica Lour. Int J Fundam Appl Sci 2012;1:87-90.
36. Bency BT, Remya K, Smitha R, Shebina PR, Azeem AK. Rotula aquatica. Lour - A review on medicinal uses phytochemistry and pharmacological actions. Indian J Pharm Biol Res 2017;5:4-9.
37. Aswathanarayan JB, Vittal RR. In vitro evaluation of antioxidant and antibacterial activities of Rotula aquatica and Ancistrocladus heyneanus: Antioxidant and antimicrobial activity of medicinal plants. J Pharm Res 2013;6:313-7.
38. Raj KB, Joseph AV. Antibacterial potential of phytochemicals against urinary tract infectious bacteria. Int J Innov Res Dev 2016;5:94-9.
39. Parekh J, Chanda S. In vitro antimicrobial activity of some Indian folklore medicinal plants. J Cell Tissue Res 2006;6:577-80.
40. Dandge PB, Karajagi AB, Bhagwat PK, Bhise KK. Phytochemical profiling and antimicrobial potential of Santalum album L, Morinda citrifolia L. And Mucuna pruriens L. Res J Life Sci Bioinform Pharm Chem Sci 2016;1:265-72.
41. Parekh J, Jadeja D, Chanda S. Efficacy of aqueous and methanol extracts of some medicinal plants for potential antibacterial activity. Turk J Biol 2006;29:203-10.
42. Sivananthan M. Antibacterial activity of 50 medicinal plants used in folk medicine. Int J Biosci 2013;3:104-21.
43. Murugan VK, Arumugam T, Tamilvannan V, Umaarani K, Dhamotharan R, Ravikumar S. Phytochemical analysis and in vitro antibacterial properties of selected medicinal plants. Eur J Pharm Med Res 2015;2:189-94.
44. Hire KK, Dhale DA. Antimicrobial effect and in silico admet prediction of Santalum album L. Int J Pharm Bio Sci 2012;3:727-34.
45. Shamsi TN, Parveen R, Afreen S, Azam M, Fatma T, Haque QM, et al. In-vitro antibacterial and antioxidant activities of sandalwood (Santalum album). Austin J Biotechnol Bioeng 2014;1:3.
46. Nyiligira E, Viljoen AM, Ba?er KH, Ozek T, Van Vuuren SF, Houghton PJ. Essential oil composition and in vitro antimicrobial and anti-inflammatory activity of South African Vitex Species. S Afr J Bot 2004;70:611-7.
47. Aswar PB, Khadabadi SS, Kuchekar BS, Rajurkar RM, Saboo SS, Javarkar RD. In-vitro evaluation of anti-bacterial and anti-fungal activity of Vitex nigundo (Verbenaceae). Ethnobotanical Leaflets 2009;2009:13.
48. Menghani E, Pareek A, Negi RS, Ojha CK. Search for antimicrobial potentials from certain Indian medicinal plants. Res J Med Plants 2011;5:295-301.
49. Ahmad N, Khan MI, Ahmed S, Javed SB, Faisal M, Anis M, et al. Change in total phenolic content and antibacterial activity in regenerants of Vitex negundo L. Acta Physiol Plant 2013;35:791-800.
50. Thombre R, Jagtap R, Patil N. Evaluation of phytoconstituents, antibacterial, antioxidant and cytotoxic activity of Vitex negundo L. and Tabernaemontana divaricata L. Int J Pharm Bio Sci 2013;4:389-96.
51. Prashith KT, Manasa M, Poornima G, Abhipsa V, Rekha C, Upashe SP, et al. Antibacterial, cytotoxic and antioxidant potential of Vitex negundo Var. Negundo and Vitex negundo Var. Purpurascens - A comparative study. Sci Technol Arts Res J 2013;2:59-68.
52. Ling TJ, Ling WW, Chen YJ, Wan XC, Xia T, Du XF, et al. Antiseptic activity and phenolic constituents of the aerial parts of Vitex negundo var. Cannabifolia. Molecules 2010;15:8469-77.
53. Silva F, Ferreira S, Queiroz JA, Domingues FC. Coriander (Coriandrum sativum L.) essential oil: Its antibacterial activity and mode of action evaluated by flow cytometry. J Med Microbiol 2011;60:1479-86.
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