SPECTROPHOTOMETRIC METHODS FOR THE QUANTIFICATION OF CARISOPRODOL USING FERRIC CHLORIDE, O-PHENANTHROLINE, AND P-NITROANILINE, SODIUM NITRITE AS ANALYTICAL REAGENTS

  • MURALI D Department of Biochemistry, Acharya Nagarjuna University, N. Nagar, Guntur, Andhra Pradesh, India.
  • PURNA CHANDRA RAO G Department of Chemistry, NRI Institute of Technology, Pothavarappadu, Agiripalli, Vijayawada, Andhra Pradesh, India.

Abstract

Objective: The present study represents the development of two spectrophotometric methods for the determination of carisoprodol (CCP) in pure and formulations using ferric chloride, o-phenanthroline, and p-nitroaniline (PNA), sodium nitrate as analytical reagents.


Methods: The proposed spectrophotometric methods were developed based on oxidation of Fe3+ by CCP, and then, the resultant product was reacted with o-phenanthroline in acidic condition forms an orange-colored complex and diazotization of PNA followed by coupling with CCP in an alkaline medium forms yellow-colored complex.


Results: Under the optimized conditions, the absorbance of CCP concentration obeyed the Beer’s law in the ranges of 10–60 μg/mL with good correlation coefficient values of 0.9992 and 0.9990 with the limit of detection values of 1.286 and 2.408 μg/mL, respectively.


Conclusion: The proposed methods were successfully applied for the determination of CCP in pure and in their formulations.

Keywords: Ferric chloride, o-Phenanthroline, p-Nitroaniline, Sodium nitrate, Spectrophotometry, Analysis.
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How to Cite
D, M., and P. C. RAO G. “SPECTROPHOTOMETRIC METHODS FOR THE QUANTIFICATION OF CARISOPRODOL USING FERRIC CHLORIDE, O-PHENANTHROLINE, AND P-NITROANILINE, SODIUM NITRITE AS ANALYTICAL REAGENTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 2, Jan. 2019, pp. 384-90, https://innovareacademics.in/journals/index.php/ajpcr/article/view/29902.
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