IN VITRO ANTHELMINTIC ACTIVITY OF ABUTILON THEOPHRASTI MEDIK. (MALVACEAE) AGAINST EGGS AND L3 LARVAE OF HAEMONCHUS CONTORTUS

MUSHEERUL HASSAN; MIR RA; TRIPTI JOSHI; FIRDOUS AHMAD YATOO; HUMA HABIB

doi:10.22159/ajpcr.2019.v12i3.29922

Authors

MUSHEERUL HASSAN Deparment of Life Science, Pacific University, Udaipur, Rajasthan, India.
MIR RA Department of Zoology, Islamia College of Science and Commerce, Srinagar, Jammu and Kashmir, India.
TRIPTI JOSHI Department of Fire and Safety Pacific University, Udaipur, Rajasthan, India.
FIRDOUS AHMAD YATOO Department of Botany, Bhagwant University, Ajmer, Rajasthan, India.
HUMA HABIB Department of Biochemistry, Islamia College of Science and Commerce, Srinagar, Jammu and Kashmir, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i3.29922

Keywords:

Haemonchus contortus, Egg hatch test, Larval motility test

Abstract

Objective: The present study was carried out to assess the in vitro anthelmintic activity of Abutilon theophrasti stem extracts.

Methods: Simple maceration was employed for extraction. Solvents such as methanol, water, and hexane were used. Egg hatch test (EHT) and larval motility test were employed to check the anthelmintic activity of crude extracts. Concentrations of 500, 250, 125, 62.5, and 31.25 mg/ml were made. Levamisole and distilled water served as control, respectively.

Results: All selected extracts displayed concentration-dependent inhibition except aqueous extracts. At higher concentration (500 mg/ml), stem extracts (methanol, aqueous, and ethanol) showed 74.39%, 72.5 and 70.03% of efficacy in EHT, respectively (p≤0.05). Meanwhile, inhibition of larval motility was seen higher with inhibition percentage of 79.79, 75.65, and 71.28 by methanolic, aqueous, and hexane extracts, respectively (p≤ 0.05).

Conclusion: The presents study suggests active principles having anthelmintic efficacy in Abutilon theopharsti stem.

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Author Biographies

MUSHEERUL HASSAN, Deparment of Life Science, Pacific University, Udaipur, Rajasthan, India.

Phd Scholar

TRIPTI JOSHI, Department of Fire and Safety Pacific University, Udaipur, Rajasthan, India.

Department of Zoology

Rank: Assiatant Professor

FIRDOUS AHMAD YATOO, Department of Botany, Bhagwant University, Ajmer, Rajasthan, India.

Department of Biochemistry,

Rank: Assiatant Professor.

HUMA HABIB, Department of Biochemistry, Islamia College of Science and Commerce, Srinagar, Jammu and Kashmir, India.

Department of Fire and safety

Rank: Assiatant Professor

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