IN VITRO ANTHELMINTIC ACTIVITY OF ABUTILON THEOPHRASTI MEDIK. (MALVACEAE) AGAINST EGGS AND L3 LARVAE OF HAEMONCHUS CONTORTUS
Keywords:Haemonchus contortus, Egg hatch test, Larval motility test
Objective: The present study was carried out to assess the in vitro anthelmintic activity of Abutilon theophrasti stem extracts.
Methods: Simple maceration was employed for extraction. Solvents such as methanol, water, and hexane were used. Egg hatch test (EHT) and larval motility test were employed to check the anthelmintic activity of crude extracts. Concentrations of 500, 250, 125, 62.5, and 31.25 mg/ml were made. Levamisole and distilled water served as control, respectively.
Results: All selected extracts displayed concentration-dependent inhibition except aqueous extracts. At higher concentration (500 mg/ml), stem extracts (methanol, aqueous, and ethanol) showed 74.39%, 72.5 and 70.03% of efficacy in EHT, respectively (p≤0.05). Meanwhile, inhibition of larval motility was seen higher with inhibition percentage of 79.79, 75.65, and 71.28 by methanolic, aqueous, and hexane extracts, respectively (p≤ 0.05).
Conclusion: The presents study suggests active principles having anthelmintic efficacy in Abutilon theopharsti stem.
Dhar DN, Sharma RL, Bansal GC. Gastro-intestinal nematodes in sheep in Kashmir. Vet Para 1982;11:271-7.
Kundu SU, Roy SA, Nandi SU, Ukil BI, Lyndem LM. In vitro anthelmintic effects of Senna occidentalis (L.) link (Leguminosae) on rat tapeworm Hymenolepis diminuta. Int J Pharm Pharm Sci 2015;7:286-71.
Ueno H, Gonçalves PC. Manual Para Diagnóstico das Helmintoses de Ruminantes. Tokyo: Japan International Cooperation Agency; 1998.
Coles GC, Bauer C, Borgsteede FH, Geerts S, Klei TR, Taylor MA, et al. World association for the advancement of veterinary parasitology (WAAVP) methods for the detection of anthelmintic resistance in nematodes of veterinary importance. Vet Para 1992;44:35-44.
Paszkowski WL, Kremer RJ. Biological activity and tentative identification of flavonoid components in velvetleaf (Abutilon theophrasti Medik.) seed coats. J Chem Eco 1988;14:1573-82.
Matławska I, Sikorska M. Flavonoids from Abutilon theophrasti flowers. Acta Pol Pharm 2005;62:135-9.
Dinu M, Uivarosi V, Popescu L, Radulescu V, Arama CC, Nicolescu TO, et al. Proximate composition and some physico-chemical properties of Abutilon theophrasti (velvetleaf) seed oil. Rev de Chim (Bucuresti) 2010;61:50-4.
Balah MA, Nassar MI. Allelopathic constituents from Abutilon theophrasti aerial parts to other weeds. Res J Agric Biol Sci 2011;7:243-50.
Kiyamova SE, Khidyrova NK, Shakhidoyatov KM. Neutral compounds from leaves of plants of the family Malvaceae. II. Hibiscus tronuma A. Chem Nat Comp 2012;1:1-2.
Tian C, Wang M, Liu X, Wang H, Zhao C. HPLC quantification of nine chemical constituents from the five parts of Abutilon theophrasti Medic. J Chro Sci 2013;10:258-63.
Jones CG, Lawton JH, Shachak M. Organisms as ecosystem engineers. In: Ecosystem Management. New York, NY: Springer; 1994
Haslam E. Natural polyphenols (vegetable tannins) as drugs: Possible modes of action. J Nat Prod 1996;59:205-15.
Aerts RJ, Barry TN, McNabb WC. Polyphenols and agriculture: Beneficial effects of proanthocyanidins in forages. Agric Econ Environ 1999;75:1-2.
Niezen JH, Waghorn GC, Graham T, Carter JL, Leathwick DM. The effect of diet fed to lambs on the subsequent development of Trichostrongylus colubriformis larvae in vitro and on pasture. Vet Par 2002;7:269-83.
Baby AA, Raphael KR. Antioxidant and anthelmintic potential of the stem and leaves of white abrus. Int J Pharm Pharm Sci 2014;5:126-9.
Hassan M, Reyaz AM, Huma H. In-vitro anthelmintic activity of different parts (leaf and root) of Abutilon theopharsti. World J Pharm Pharm Sci 2016;5:738-46.
Grady J, Kotze AC. Haemonchus contortus: In vitro drug screening assays with the adult life stage. Exp Para 2004;106:164-72.
Powers KG, Wood IB, Eckert J, Gibson T, Smith HJ. World association for the advancement of veterinary parasitology (WAAVP) guidelines for evaluating the efficacy of anthelmintics in ruminants (bovine and ovine). Vet Para 1982;10:265-84.
Rodriguez A, Vigorito E, Clare S, Warren MV, Couttet P, Soond DR, et al. Requirement of bic/microRNA-155 for normal immune function. Sciences 2007;316:608-11.
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