NEUROPROTECTION OF ABELMOSCHUS ESCULENTUS L. AGAINST DIABETIC NEUROPATHY

  • Lee Wei Yang Departement of Pharmacology, School of Pharmacy, Management and Science University, Shah Alam Selangor, Malaysia.
  • Santosh Fattepur Departement of Pharmacology, School of Pharmacy, Management and Science University, Shah Alam Selangor, Malaysia.
  • Kiran Chanabasappa Nilugal Departement of Pharmacology, School of Pharmacy, Management and Science University, Shah Alam Selangor, Malaysia.
  • Fadli Asmani Department of Clinical pharmacy, School of Pharmacy, Management and Science University Shah Alam, Selangor, Malaysia.
  • Eddy Yusuf Department of Clinical pharmacy, School of Pharmacy, Management and Science University Shah Alam, Selangor, Malaysia.
  • Mohd Nizam Abdul Ghani Department of Clinical pharmacy, School of Pharmacy, Management and Science University Shah Alam, Selangor, Malaysia.
  • Ibrahim Abdullah Department of Clinical pharmacy, School of Pharmacy, Management and Science University Shah Alam, Selangor, Malaysia.

Abstract

Objective: The present study was designed to determine the neuroprotective effect of Abelmoschus esculentus L. on alloxan-induced diabetic neuropathy in rats.

Methods: Diabetes was induced in rats with a single intraperitoneal injection of alloxan monohydrate (130 mg/kg b.w). The ethanol extract of A. esculentus L. at a dose of 100 and 200 mg/kg of body weight was administered at single dose per day to alloxan-induced diabetic rats for 21 days. The fasting blood glucose was screened in the intermittent on day 0, day 14, and day 21. Behavioral tests such as thermal hyperalgesia test and rotarod performance test were performed to assess the thermal sensitivity and muscle grip strength. At the end of the study period, experimental animals were sacrificed and sciatic nerve tissues were obtained for histopathological investigation.

Results: Animals treated with A. esculentus L. extarct at a dose of 200 mg/kg of body weight significantly reduced (p<0.05) in hyperglycemia and thermal hyperalgesia and significantly increased (p<0.05) in rotarod performance. The sciatic nerve fiber of diabetic rats receiving 200 mg/kg of body weight of A. esculentus L. extract also shows no swelling of nerve fibers, and lesser demyelination was observed.

Conclusion: These findings demonstrate that A. esculentus L. exhibits significant antidiabetic and neuroprotective effect against alloxan-induced diabetic neuropathy in rats.

Keywords: Abelmoschus esculentus L, Neuroprotective, Antidiabetic, Histopathological investigation.

References

1. International Diabetes Federation. Diabetes in Malaysia-2014; 2014. Available from: https://www.idf.org/sites/default/files/Atlas-poster-2014_EN.pdf. [Last accessed on 2014 Jun].
2. The Expert Committee on the Diagnosis and Classification of Diabetes Mellitus. report of the expert committee on the diagnosis and classification of diabetes mellitus. Diabetes Care 2003;36 Suppl 2:1183-97.
3. Forbes JM, Cooper ME. Mechanisms of diabetic complications. Physiol Rev 2011;93:137-88.
4. Boulton AJ, Malik RA. Diabetic neuropathy. Med Clin North Am 1998;82:909-29.
5. Tesfaye S, Selvarajah D. Advances in the epidemiology, pathogenesis and management of diabetic peripheral neuropathy. Diabetes Metab Res Rev 2012;1:8-14.
6. Kasper D, Fauci A, Hauser S, Longo D, Jameson J, Loscalzo J. Harrison’s Principles of Internal Medicine. 19th ed. New York: McGraw- Hill, Medical Pub. Division; 2015.
7. Negi G, Kumar A, Joshi RP, Ruby PK, Sharma SS. Oxidative stress and diabetic neuropathy: Current status of antioxidants. Inst Integrat Omics Appl Biotechnol 2011;2:71-8.
8. Pourmand R. Diabetic neuropathy. Neurol Clin 1997;3:569-76.
9. Edwards JL, Vincent AM, Cheng HT, Feldman EL. Diabetic neuropathy: Mechanisms to managemen. Pharmacol Ther 2008;120:1-34.
10. Yagihashi S, Mizukami H, Sugimoto K. “Mechanism of diabetic neuropathy: Where are we now and where to go? J Diabetes Invest 2011;2:18-32.
11. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal Plants. New Delhi: Council of Industrial and Scientific Research; 1956. p. 1-133.
12. Ndunguru J, Rajabu AC. Effect of okra mosaic virus disease on the above-ground morphological yield components of okra in Tanzania. Sci Horticul 2004;99:225-35.
13. Gemede HF, Ratta N, Haki GD, Woldegiorgis AZ, Beyene F. Nutritional quality and health benefits of okra (Abelmoschus esculentus): A review. Food Sci Q Manage 2015. DOI: 10.4172/2157-7110.1000458.
14. Subrahmanyam GV, Sushma M, Alekya A, Neeraja CH, Harsha HS, Ravindra J. Antidiabetic activity of Abelmoschus esculentus fruit extract. Int J Res Pharm Chem 2011;1:17-20.
15. Ngoc TH, Ngo QN, Van AT, Phung NV. Hypolipidemic effect of extracts from Abelmoschus esculentus L. (Malvaceae) on tyloxapol-induced hyperlipidemia in mice. Warasan Phesatchasat 2008;35:42-6.
16. Sabitha V, Ramachandran S, Naveen KR, Panneerselvam K. Investigation of in vivo antioxidant property of Abelmoschus esculentus (L). Oench. fruit seed and peel powders in streptozotocin-induced diabetic rats. J Ayurveda Integr Med 2012;3:188-93.
17. Pendre NK, Nema PK, Sharma HP, Rathore SS, Kushwah SS. Effect of drying temperature and slice size on quality of dried okra (Abelmoschus esculentus (L.) Moench). J Food Sci Technol 2012;49:378-81.
18. Raju S, Hemamalini K. In vivo animal model for screening of antidiabetic activity. Asian J Pharm Clin Res 2012;5:118-24.
19. Austin PJ, Wu A, Moalem-Taylor G. Chronic constriction of the sciatic nerve and pain hypersensitivity testing in rats. J Vis Exp 2012;61:3393.
20. Deacon RM. Measuring motor coordination in mice. J Vis Exp 2013;75:2609.
21. Rohilla A, Ali S. Alloxan induced diabetes: Mechanisms and effects. Int J Res Pharm Biomed Sci 2012;3:819-23.
Statistics
281 Views | 149 Downloads
How to Cite
Yang, L. W., S. Fattepur, K. C. Nilugal, F. Asmani, E. Yusuf, M. N. A. Ghani, and I. Abdullah. “NEUROPROTECTION OF ABELMOSCHUS ESCULENTUS L. AGAINST DIABETIC NEUROPATHY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 11, no. 15, Oct. 2018, pp. 28-31, doi:10.22159/ajpcr.2018.v11s3.30023.
Section
Original Article(s)