PREPARATION OF SOLID LIPID NANOPARTICLES CONTAINING MANGOSTEEN PERICARP EXTRACT


Siti Nur Diniyanti

Abstract


Objectives: The aim of this study was to develop solid lipid nanoparticles (SLNs) containing mangosteen pericarp extract (MPE) to achieve enhanced photoprotection and to provide an alternative to synthetic sunscreens in the market.

Materials and Methods: The MPE was prepared using the maceration method, and evaluated for sun protection factor (SPF) value using an ultraviolet (UV)-Vis spectrophotometer. SLNs were prepared through ultrasonication method. Blank-SLNs were formulated using stearic acid (SA) or palmitic acid (PA) as solid lipids at a concentration of 3%. Tween® 80 or polyvinyl alcohol (PVA) was employed as a surfactant with a concentration ranging from 1 to 2%. The obtained blank-SLNs were investigated for their physical characteristics, (i.e., morphology, particle size, polydispersity index [PDI], and zeta potential values). The blank-SLNs with suitable physical characteristics were selected to encapsulate MPE and evaluated for the physical characteristics.

Results: The MPE was a brownish viscous substance with an SPF value that ranged from 3.09±0.005 to 27.20±0.05 at a concentration ranging from 0.02 to 0.1 mg/ml. Based on the physical characteristics, the blank-SLNs employing PA or SA with 1% of PVA were selected. The MPE-SLNs were spherical, with a particle size that ranged from 443.51±6.50 to 533.52±16.15 nm; PDI ranged from 0.35±0.008 to 0.459±0.02, and zeta potential value ranged from 18.32±1.37 to −19.03±0.64. The entrapment efficiencies of MPE-PA-SLNs and MPE-SA-SLNs were 83.24±1.37% and 84.17±0.411%, respectively.

Conclusion: The results indicated the promising potential of MPE as a UVB photoprotector. The MPE-SLNs were also successfully formulated, but, further study is needed to confirm the potential of MPE-SLNs to be used as a sunscreen, and their stability during storage.


Keywords


Mangosteen pericarp extract, Solid lipid nanoparticles (SLNs), Ultraviolet radiation, Sun protection factor.

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References


Balogh TS, Velasco MV, Pedriali CA, Kaneko TM, Baby AR. Ultraviolet radiation protection: Current available resources in photoprotection. An Bras Dermatol 2011;86:732-42.

Saewan N, Jimtaisong A. Photoprotection of natural flavonoids. J Appl Pharm Sci 2013;3:129-41.

González S, Fernández-Lorente M, Gilaberte-Calzada Y. The latest on skin photoprotection. Clin Dermatol 2008;26:614-26.

Shannon MA, Bohn PW, Elimelech M, Georgiadis JG, Mariñas BJ, Mayes AM, et al. Science and technology for water purification in the coming decades. Nature 2008;452:301-10.

Khazaeli P, Mehrabani M. Screening of sun protective activity of the ethyl acetate extracts of some medicinal plants. Iran J Pharm Res 2010;7:5-9.

Pathirana R, Ratnasooriya W, Gamage R, Hasanthi K, Hettihewa S. Sunscreen activity of pericarp of fruit of Sri Lankan Garcinia mangostana L.(Mangosteen) in vitro. Imp J Interdiscip Res 2016;3:2225-9.

Yodhnu S, Sirikatitham A, Wattanapiromsakul C. Validation of LC for the determination of α-mangostin in mangosteen peel extract: A tool for quality assessment of Garcinia mangostana L. J Chromatogr Sci 2009;47:185-9.

Sanad RA, Abdel Malak NS, El-Bayoomy TS, Badawi AA. Preparation and characterization of oxybenzone-loaded solid lipid nanoparticles (SLNs) with enhanced safety and sunscreening efficacy: SPF and UVA-PF. Drug Discov Ther 2010;4:472-83.

Wissing S, Müller R. Solid lipid nanoparticles as carrier for sunscreens: In vitro release and in vivo skin penetration. J Control Release 2002;81:225-33.

Severino P, Moraes LF, Zanchetta B, Souto EB, Santana MH. Elastic liposomes containing benzophenone-3 for sun protection factor enhancement. Pharm Dev Technol 2012;17:661-5.

Mansur M, Leitão S, Cerqueira-Coutinho C, Vermelho A, Silva R, Presgrave O, et al. In vitro and in vivo evaluation of efficacy and safety of photoprotective formulations containing antioxidant extracts. Rev Bras Farmacogn 2016;26:251-8.

Gaikwad M, Kale S. Formulation and in vitro evaluation for sun protection factor of Moringa oleifera Lam (family-Moringaceae) oil sunscreen cream. Int J Pharm Pharm Sci 2011;3:371-5.

Al-Qushawi A, Rassouli A, Atyabi F, Peighambari SM, Esfandyari- Manesh M, Shams GR, et al. Preparation and characterization of three tilmicosin-loaded lipid nanoparticles: Physicochemical properties and in-vitro antibacterial activities. Iran J Pharm Res 2016;15:663-76.

Aisha A, Abu-Salah K, Ismail Z, Majid A. Determination of total xanthones in Garcinia mangostana fruit rind extracts by ultraviolet (UV) spectrophotometry. J Med Plants Res 2013;7:29-35.

Jujun P, Pootakham K, Pongpaibul Y, Tharavichitkul P, Ampasavate C. HPLC determination of mangostin and its application to storage stability study. CMU J Nat Sci 2009;8:43-53.

Mansur J, Breder M, Mansur M, Azulay R. Determinação do fator de proteção solar por espectrofotometria. An Bras Dermatol 1986;61:121-4.

Sayre RM, Agin PP, LeVee GJ, Marlowe E. A comparison of in vivo and in vitro testing of sunscreening formulas. Photochem Photobiol 1979;29:559-66.

Bhalekar M, Upadhaya P, Madgulkar A. Formulation and characterization of solid lipid nanoparticles for an anti-retroviral drug darunavir. Appl Nanosci 2017;7:47-57.

Xie S, Zhu L, Dong Z, Wang X, Wang Y, Li X, et al. Preparation, characterization and pharmacokinetics of enrofloxacin-loaded solid lipid nanoparticles: Influences of fatty acids. Colloids Surf B Biointerfaces 2011;83:382-7.

Medina-Holguín A, Micheletto S, Holguín F, Rodriguez J, O’Connell M, Martin C. Environmental influences on essential oils in roots of Anemopsis californica. HortScience 2007;42:1578-83.

Fitzpatrick TB. The validity and practicality of sun-reactive skin types I through VI. Arch Dermatol 1988;124:869-71.

Hayakawa I, Kanno T, Yoshiyama K, Fujio Y. Oscillatory compared with continuous high pressure sterilization on Bacillus stearothermophilus spores. J Food Sci 1994;59:164-7.

Park JW, Takahata Y, Kajiuchi T, Akehata T. Effects of nonionic surfactant on enzymatic hydrolysis of used newspaper. Biotechnol Bioeng 1992;39:117-20.

Turk CT, Oz UC, Serim TM, Hascicek C. Formulation and optimization of nonionic surfactants emulsified nimesulide-loaded PLGA-based nanoparticles by design of experiments. AAPS PharmSciTech 2014;15:161-76.

Zhang Z, Tan S, Feng SS. Vitamin E TPGS as a molecular biomaterial for drug delivery. Biomaterials 2012;33:4889-906.

Rawat MK, Jain A, Mishra A, Muthu MS, Singh S. Development of repaglinide loaded solid lipid nanocarrier: Selection of fabrication method. Curr Drug Deliv 2010;7:44-50.

Mohanty B, Majumdar DK, Mishra SK, Panda AK, Patnaik S. Development and characterization of itraconazole-loaded solid lipid nanoparticles for ocular delivery. Pharm Dev Technol 2015;20:458-64.

Rowe R, Sheskey P, Weller P. Handbook of Pharmaceutical Excipients. 6th ed. London: Pharmaceutical Press London; 2006.

Weiss J, Decker E, McClements D, Kristbergsson K, Helgason T, Awad T. Solid lipid nanoparticles as delivery systems for bioactive food components. Food Biophys 2008;3:146-54.

Wulff-Pérez M, Torcello-Gómez A, Gálvez-Ruíz M, Martín Rodríguez A. Stability of emulsions for parenteral feeding: preparation and characterization of o/w nanoemulsions with natural oils and Pluronic f68 as surfactant. Food Hydrocoll 2009;23:1096-102.




About this article

Title

PREPARATION OF SOLID LIPID NANOPARTICLES CONTAINING MANGOSTEEN PERICARP EXTRACT

Keywords

Mangosteen pericarp extract, Solid lipid nanoparticles (SLNs), Ultraviolet radiation, Sun protection factor.

DOI

10.22159/ajpcr.2018.v11s3.30040

Date

06-10-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Special issue 3 2018 Page: 80-84

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Siti Nur Diniyanti
Department of Pharmaceutical Technology, Chulalongkorn University, Thailand.
Thailand


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