CULTURED HAIR FOLLICLE CELLS FOR THE TREATMENT OF VITILIGO


Piyatida Sutnut, Saroj Suvanasuthi, Kwanchanok Viravaidya-pasuwat

Abstract


Objective: The objective of this study was to develop a method to isolate cells from hair follicles and cultured them in a hydrogel.

Methods: Different cell types obtained from hair follicles were investigated and mixed with three formulations of Lutrol® F-127-based hydrogels. The percentages of the cell attachment and viability were observed within 48 h.

Results: The results showed that three cell types, including keratinocyte, dermal papilla, and melanocyte cells, were obtained, as shown by the expression of their corresponding genes. All formulations of the hydrogels supported cell attachment and viability. Interestingly, more than 60% cell attachment and viability were found in lutrol hydrogels supplemented with either fetal bovine serum (FBS) or heat-activated human serum.

Conclusion: Higher cell attachment and viability were observed when the hair follicle cells were cultured in the hydrogel with FBS than the hydrogel with human serum. However, the lutrol gel formulation with human serum was more appropriate to be used in the future clinical study, as this formulation contained no animal-derived component.


Keywords


Hair follicles, Hydrogel, Lutrol® F-127, Vitiligo.

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About this article

Title

CULTURED HAIR FOLLICLE CELLS FOR THE TREATMENT OF VITILIGO

Keywords

Hair follicles, Hydrogel, Lutrol® F-127, Vitiligo.

DOI

10.22159/ajpcr.2018.v11s3.30043

Date

06-10-2018

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 11 Special issue 3 2018 Page: 94-97

Print ISSN

0974-2441

Online ISSN

2455-3891

Authors & Affiliations

Piyatida Sutnut
Department of Biological Engineering Program, Faculty of Engineering, KMUTT, Bangkok, Thailand.
Thailand

Saroj Suvanasuthi
Department of Samitivej Sukhumvit Hospital, 33 Sukhumvit 49, Klongtan Nua, Vaghana, Bangkok, Thailand.
Thailand

Kwanchanok Viravaidya-pasuwat
Department of Biological Engineering Program, Faculty of Engineering, KMUTT, Bangkok, Thailand.
Thailand


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