EFFECT OF AQUEOUS EXTRACT OF CITRULLUS LANATUS PULP ON SCOPOLAMINE INDUCED COGNITIVE DYSFUNCTION IN SWISS ALBINO MICE
Objective: Amnesia is a medical condition involving the loss of memory. The memory loss is attributed to a number of illnesses or factors including Alzheimer’s disease (AD) and dementia, amnesia is often caused by head injury, brain trauma, or brain surgery. The incidence of amnesia affects only a small percent of the world’s population, the relevant study is becoming increasingly important with the rising numbers of people with AD. Alzheimer’s is predicted to strike 34 million people globally by 2025 and 14 million within the U.S. alone over subsequent 40 years.
Methods: The study the possible protective effect of aqueous extract of Citrullus lanatus pulp (ACL) using scopolamine-induced amnesia in Swiss Albino mice. The mice were divided randomly into six groups each of five mice (n=5). Groups IV, V, and VI received mice feed and ACL 25%, 50%, and 100% instead of water for 21 days. Mice of Groups I, II, and III were treated with mice feed and water ad libitum. The animals were subjected to a single dose of scopolamine (1 mg/kg b.wt. ip) except in Group I on the 21st day 60 min after respective drug administration and observed for the effects in brain activity for the learning and memory. The behavioral parameters such as passive avoidance, Morris water maze, Y-Maze, and elevated plus maze were used as a tool for cognitive dysfunction study.
Results: The ACL significantly reversed the scopolamine induced amnesia in mice. This is evident as C. lanatus is rich in lycopene content. The experimental models demonstrated that all the concentration of ACL treated mice showed remarkable results in restoring the effect of amnesia induced by scopolamine.
Conclusion: To concise, these results suggest that ACL may exhibit protective effect on the brain to reverse the scopolamine induced amnesia. Further, it can be explored extracting the lycopene content of watermelon.
2. Whitehouse PJ, Price DL, Clark AW, Coyle JT, DeLong MR. Alzheimer disease: Evidence for selective loss of cholinergic neurons in the nucleus basalis. Ann Neurol 1982;10:122-6.
3. Klinkenberg I, Blokland A. The validity of scopolamine as a pharmacological model for cognitive impairment: A review of animal behavioral studies. Neurosci Biobehav Rev 2010;34:50.
4. Imam A, Ajao MS, Ajibola MI, Amin A, Abdulmajeed WI, Lawal AZ, et al. Black seed oil ameliomiceed scopolamine-induced memory dysfunction and cortico-hippocampal neural alterations in male Wistar mices. Bull Fac Pharm Cairo Univ 2016;54:49-57.
5. Bores GM, Huger FP, Petko W, Mutlib AE, Camacho F, Rush DK, et al. Pharmacological evaluation of novel Alzheimer’s disease therapeutics: Acetylcholinesterase inhibitors related to galantamine. J Pharmacol Exp Ther 1996;277:728-38.
6. Allain H, Bentue-Ferrer D, Tribut O, Gauthier S, Michel BF, Drieu-La RC. Alzheimer’s disease: The pharmacological pathway. Fundam Clin Pharmacol 2003;17:419-28.
7. Vaghela B, Buddhadev S, Shukla L. Pharmacological activities of Desmodium gangeticum: An overview. An Int J Pharm Sci 2013;4:264-78.
8. Yativ M, Harary I, Wolf S. Sucrose accumulation in watermelon fruits: Genetic variation and biochemical analysis. J Plant Physiol 2010;167:589-96.
9. Adunola AT, Chidimma AL, Olatunde DS, Peter OA. Antibacterial activity of watermelon (Citrullus lanatus) seed against selected microorganisms. Afr J Biotechnol 2015;14:1224-9.
10. Hassan LE. In vitro antigiardial activity of Citrullus lanatus var. citroides extracts and cucurbitaceous isolated compounds. J Med Plants Res 2011;5:3338-46.
11. Madhavi P, Vakati K, Rahman H. Hepatoprotective activity of Citrullus lanatus seed oil on ccl4 induced liver damage in rats. SAJP 2012;1:30-3.
12. Bhardwaj A, Kumar R, Dabas V, Alam N. Evaluation of anti-ulcer activity of Citrullus lanatus seed extract in wistar albino rats. Int J Pharm Pharm Sci 2012;4:135-9.
13. Jiyun A, Wonhee C, Suna K, Taeyoul H. Anti-diabetic effect of watermelon (Citrullus vulgaris Schrad) on Streptozotocin-induced diabetic mice. Food Sci Biotechnol 2011;20:251-4.
14. Sharma S. First report on laxative activity of Citrullus lanatus. Pharmacol Online 2011;2:790-7.
15. Olamide AA, Olayemi OO, Demetrius OO, Olatoye OJ, Kehinde AA. Effects of methanolic extract of Citrullus lanatus seed on experimentally induced prostatic hyperplasia. Eur J Med Plants 2011;1:171-9.
16. Gill NS. Evaluation of antioxidant activity of Citrullus lanatus seed extract in rats. Lat Am J Pharm 2011;30:429.
17. Kumari A, Rao J, Kumari J. Analgesic activity of aqueous extract of Citrullus lanatus peels. Advin Pharmacol Pharma 2013;1:135-8.
18. Abdelwahab I, Hassan LE, Hasnah MS, Yagi SM, Koko WS, Mohan S, et al. Anti-inflammatory activities of Cucurbitacin E isolated from Citrullus lanatus var. citrodies: Role of reactive nitrogen species and cyclooxygenase enzyme inhibition. Fitoterapia 2011;82:1190-7.
19. Oyenihi OR, Afolabi BA, Oyenihi AB, Ogunmokun OJ, Oguntibeju OO. Hepato-and neuro-protective effects of watermelon juice on acute ethanol-induced oxidative stress in mices. Toxicol Rep 2016;3:288-94.
20. Mabberley DI. The Plant Book. Cambridge, New York: Cambridge Cambridge University Press; 1987.
21. Oluwole FS, Balogun ME. Antisecretory effects of watermelon (Citrullus lanatus) juice in male albino mices. Ann Revi Res Biol 2013;3:358-66.
22. Mohammad MK, Mohamed MI, Zakaria AM, Razak HR, Saad WM. Watermelon (Citrullus lanatus (Thunb.) Matsum. and Nakai) juice modulates oxidative damage induced by low dose x-ray in mice. Biomed Res Int 2014;2014:512834.
23. Ohno M, Sametsky EA, Younkin LH, Oakley H, Younkin SG, Citron M, et al. BACE1 deficiency rescues memory deficits and cholinergic dysfunction in a mouse model of Alzheimer’s disease. Neuron 2004;41:27-33.
24. Dhingra D, Parle M, Kulkarni SK. Memory enhancing activity of Glycyrrhiza glabra in mice. J Ethnopharmacol 2004;91:361-5.
25. Itoh J, Nabeshima T, Kameyama T. Utility of an elevated plus-maze for the evaluation of memory in mice: Effects of nootropics, scopolamine and electroconvulsive shock. Psychopharmacology 1990;101:27-33.
26. Parle M, Dhingra D. Ascorbic acid: A promising memory-enhancer in mice. J Pharmacol Sci 2003;93:129-35.
27. Morris R. Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 1984;11:47-60.
28. Cummings JL, Cole G. Alzheimer’s disease. JAMA 2002;287:2335-48.
29. Kang SY, Lee KY, Koo KA, Yoon JS, Lima SW, Kima YC, et al. ESP-102, a standardized combined extract of Angelica gigas, Saururus chinensis and Schizandra chinensis, significantly improved scopolamine-induced memory impairment in mice. Life Sci 2005;76:1691-705.
30. Charoensiri R, Kongkachuichai R, Suknicom S, Sungpuag P. Beta-carotene, lycopene, and alpha-tocopherol contents of selected Thai fruits. Food Chem 2009;113:202-7.
31. Chaturvedi P, Pipedi-Tshekiso M, Tumed A. Supplementation with watermelon renders protection against toxicity induced by paracetamol in albino rats: The mutual and fine interaction of antioxidants prevented the cellular damage. Int J Food Agric Vet Sci 2014;4:102-11.
32. Balahoro?lu R, Dülger H, Özbek H, Bayram ?, ?ekero?lu MR. Protective effects of antioxidants on the experimental liver and kidney toxicity in mice. Eur J Gen Med 2009;5:157-64.
33. Seif HS. Ameliorative effect of pumpkin oil (Cucurbita pepo L.) against alcohol-induced hepatotoxicity and oxidative stress in albino rats. Beni Suef Univ J Basic Appl Sci 2014;3:178-85.
34. Kasdallah-Grissa A, Mornagui B, Aouani E, Hammami M, El May M, Gharbi N, et al. Resveratrol, a red wine polyphenol, attenuates ethanol-induced oxidative stress in rat liver? Life Sci 2007;80:1033-9.
35. Naz A, Butt MS, Pasha I, Nawaz H. Antioxidant indices of watermelon juice and lycopene extract. Pak J Nutr 2013;12:255-60.
36. Zakaria AM, Ghazali N, Mohammad MK, Mohamed MI, Isa MM, Razak HR, et al. Radioprotective effect of watermelon juice against low dose ionizing radiation-induced inflammatory response in mice. World J Med Sci 2014;10:191-7.
37. Lian F, Wang XD. Enzymatic metabolites of lycopene induce Nrf2- mediated expression of phase II detoxifying/antioxidant enzymes in human bronchial epithelial cells. Int J Cancer 2008;123:1262-8.
38. Dementia and Cognitive Impairment Diagnosis and Treatment Guideline; ???.
39. Kaur R, Parveen S, Mehan S, Khanna D, Kalra S. Neuroprotective effect of ellagic acid against chronically scopolamine induced Alzheimer’s type memory and cognitive dysfunctions: Possible behavioural and biochemical evidences. Int J Preven Med Res 2015;1:45-64.
40. Available from: http://www.sbfnl.stanford.edu/bml_passive.html.
41. Kameyama T, Nabeshima T, Kozawa T. Step-down-type passive avoidance-and escape-learning method: Suitability for experimental amnesia models. J Pharmacol Methods 1986;16:39-52.
42. Zhang O, Wang Q, Chen H. Involvement of cholinergic dysfunction and oxidative damage in the effects of simulated weightlessness on learning and memory in rats. Biomed Res Int 2018;2018:2547532.
43. Swonger AK, Rech RH. Serotonergic and cholinergic involvement in habituation of activity and spontaneous alternation of rats in a Y maze. J Comp Physiol Psychol 1972;81:509-22.
44. Bala R, Khanna D, Mehan S, Kalra S. Experimental evidence for the potential of lycopene in the management of scopolamine induced amnesia. RSC Adv 2015;5:72881-92.
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