ESTIMATION THE ANTIBACTERIAL EFFECT OF CURCUMIN AND ROSEMARY AMONG PATIENTS WITH DENTAL CARIES IN VITRO
Keywords:Curcumin, Rosemary, Dental caries
Objective: The aim is to estimate the effect of curcumin and rosemary as antibacterial agents among dental caries cases.
Methods: Samples of saliva were randomly collected from 40 patients in Al-Furat General Hospital who attended the hospital from July to September 2018. Swabs were cultured on blood agar at 37°C for 24 h and then subcultured in mannitol salt agar and trypticase soy broth at 37°C for 24 h. Different concentrations of aqueous extract curcumin solution (0.1, 0.3, 0.5, and 1 mg/ml) and rosemary solution (1 g/ml) were prepared and added to the bacterial culture. Later, minimum inhibition zones of the bacterial cultures were determined.
Results: The results showed that there were 25 cases of Streptococcus mutans, 10 cases of Staphylococcus aureus, 3 cases of anaerobic bacteria, and 2 cases of normal flora among 40 culturing swabs of bacteria. Aqueous extract of curcumin showed antibacterial effect with concentrations (0.1, 0.3, 0.5, and 1 mg/ml) against oral bacteria; nevertheless, these bacteria were resistant to the aqueous extract of rosemary with concentration 1 g/ml.
Conclusion: It can be concluded that curcumin can be an effective antibacterial agent against dental caries disease and its effect increases positively in relation to its concentration. On the other hand, rosemary with 1 g/ml concentration did not show any effect on oral bacteria.
Murray CJ, Vos T, Lozano R, Naghavi M, Flaxman AD, Michaud C, et al. Disability-adjusted life years (DALYs) for 291 diseases and injuries in 21 regions, 1990-2010: A systematic analysis for the global burden of disease study 2010. Lancet 2012;380:2197-223.
Loesche WJ. Microbiology of dental decay and periodontal disease. In: Baron S, editor. Medical Microbiology. 4th ed. Galveston (TX): University of Texas Medical Branch at Galveston; 1996.
Busri A, Puspitawati R, Utami S. Antibacterial effect of java turmeric ethanol extract against dual-species Streptococcus mutans and Streptococcus sanguinis Biofilm (in vitro). Asian J Pharm Clin Res 2017;10:23087.
Rukayadi Y, Hwang JK. In vitro activity of xanthorrhizol against Streptococcus mutans biofilms. Lett Appl Microbiol 2006;42:400-4.
Eley BM. Antibacterial agents in the control of supragingival plaque – a review. Br Dent J 1999;186:286-96.
Ammon HP, Wahl MA. Pharmacology of curcuma longa. Planta Med 1991;57:1-7.
National Toxicology Program. NTP toxicology and carcinogenesis studies of turmeric oleoresin (CAS no 8024-37-1) (Major component 79%-85% curcumin, CAS no 458-37-7) in F344/N rats and B6C3F1 mice (Feed studies). Natl Toxicol Program Tech Rep Ser 1993;427:1-275.
Wang YJ, Pan MH, Cheng AL, Lin LI, Ho YS, Hsieh CY, et al. Stability of curcumin in buffer solutions and characterization of its degradation products. J Pharm Biomed Anal 1997;15:1867-76.
Wahlström B, Blennow G. A study on the fate of curcumin in the rat. Acta Pharmacol Toxicol (Copenh) 1978;43:86-92.
Aggarwal BB, Kumar A, Bharti AC. Anticancer potential of curcumin: Preclinical and clinical studies. Anticancer Res 2003;23:363-98.
Abraham A, Samuel S, Mathew L. Pharmacognostic evaluation of Curcuma longa L. Rhizome and standardization of its formulation by HPLC using curcumin as marker. Int J Pharm Phytochem Res 2018;10:38-42.
Negi PS, Jayaprakasha GK, Jagan Mohan Rao L, Sakariah KK. Antibacterial activity of turmeric oil: A byproduct from curcumin manufacture. J Agric Food Chem 1999;47:4297-300.
Ramirez-Boscd A, Soler A, Gutierrez AC, Alvarez JL, Almagro EQ. Antioxidant Curcuma extracts decrease the blood lipid peroxide levels of human subjects. Age (Omaha) 1995;18:167-9.
Toda S, Miyase T, Arichi H, Tanizawa H, Takino Y. Natural antioxidants. III. Antioxidative components isolated from rhizome of Curcuma longa L. Chem Pharm Bull (Tokyo) 1985;33:1725-8.
Mehta K, Pantazis P, McQueen T, Aggarwal BB. Antiproliferative effect of curcumin (diferuloylmethane) against human breast tumor cell lines. Anticancer Drugs 1997;8:470-81.
Sharma OP. Antioxidant activity of curcumin and related compounds. Biochem Pharmacol 1976;25:1811-2.
Chaturvedi TP. Uses of turmeric in dentistry: An update. Indian J Dent Res 2009;20:107-9.
Abbas SH, Abdulridha MK, Najeb AA. Potential benefit of curcumin adjuvant therapy to the standard Helicobacter pylori eradication therapy in patients with peptic ulcer disease. Asian J Pharm Clin Res 2017;10:313-7.
C??kr?kc?? S, Mozi?og?lu E, Y?lmaz H. Biological activity of curcuminoids isolated from Curcuma longa. Rec Nat Prod 2008;2:19-24.
Lal B, Kapoor AK, Asthana OP, Agrawal PK, Prasad R, Kumar P, et al. Efficacy of curcumin in the management of chronic anterior uveitis. Phytother Res 1999;13:318-22.
Behal R, Mali AM, Gilda SS, Paradkar AR. Evaluation of local drug-delivery system containing 2% whole turmeric gel used as an adjunct to scaling and root planing in chronic periodontitis: A clinical and microbiological study. J Indian Soc Periodontol 2011;15:35-8.
Suhag A, Dixit J, Prakash D. Role of curcumin as a subgingival irrigant: A pilot study. Periodental Pract Today 2007;4:115-21.
Ulbricht C, Windsor RC, Brigham A, Bryan JK, Conquer J, Costa D, et al. An evidence-based systematic review of annatto (Bixa orellana L.) by the natural standard research collaboration. J Diet Suppl 2012;9:57-77.
Klancnik A, Guzej B, Kolar MH, Abramovic H, Mozina SS. In vitro antimicrobial and antioxidant activity of commercial rosemary extract formulations. J Food Prot 2009;72:1744-52.
Rosselló JA, Cosín R, Boscaiu M, Vicente O, Martínez I, Soriano P. Intragenomic diversity and phylogenetic systematics of wild rosemaries (Rosmarinus officinalis L. s.l., Lamiaceae) assessed by nuclear ribosomal DNA sequences (ITS). Plant Syst Evol 2006;262:1-12.
Maha FA, Hadeel WA. The effect of aqueous extract of Curcuma longa on some parameters of cytogenetic, immunity and fertility in female mice. J Coll Basic Educ 2013;19:889-97.
Ibrahim RM. Effect of aqueous extract of Rosemary officinalis on cytotoxicity of CCL4 induced albino male mice. J Biotechnol Res Cent 2018;12:10.
Abozid M, Farid HE. Protective role and antioxidant activity of aqueous extract of Rosmarinus Officinalis against trichlorocetate--induced toxicity in liver of male rats. Asian J Pharm Clin Res 2018;11:420-4.
Selwitz RH, Ismail AI, Pitts NB. Dental caries. Lancet 2007;369:51-9.
Basheer AI. Effect of alcoholic extract of rosmarinus against some type of Entrobacteriaceae. Tikrit J Pure Sci 2018;23:18-21.
Bowen WH. Dental caries – Not just holes in teeth! A perspective. Mol Oral Microbiol 2016;31:228-33.
Tanzer JM, Livingston J, Thompson AM. The microbiology of primary dental caries in humans. J Dent Educ 2001;65:1028-37.
Aday HA. Bioactivity of curcumin extract against of some pathogenic strains. Eng Tech J 2011;29:2087-92.
Song J, Choi B, Jin EJ, Yoon Y, Choi KH. Curcumin suppresses Streptococcus mutans adherence to human tooth surfaces and extracellular matrix proteins. Eur J Clin Microbiol Infect Dis 2012;31:1347-52.
Bharali DJ, Siddiqui IA, Adhami VM, Chamcheu JC, Aldahmash AM, Mukhtar H, et al. Nanoparticle delivery of natural products in the prevention and treatment of cancers: Current status and future prospects. Cancers (Basel) 2011;3:4024-45.
Al-musawi S. Folated-nanocarrier for curcumin drug delivery in breast cancer therapy. Eng Tech J 2015;3:1643-54.
Jawad AM, Allawi AK, Mufeed EH. Essential oils of rosemary as antimicrobial agent against three types of bacteria. Med J Babylon 2018;15:1-6.
Campo JD, Amiot MA, Nguyen TC. Antimicrobial effect of rosemary extracts. J Food Prot 2000;63:1359-68.
Das S, Mandal SK. Current developments on anti-inflammatory natural medicines. Asian J Pharm Clin Res 2018;11:61-5.
Ali HI, Ali DS. Evaluation of the inhibitory effect of the water, alcohol and oil extract of Rosmarinus officinalis L. In some pathogenic bacteria. Al-Muthanna J Agric 2018;6:41-7.
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