• IKRAME ZEOUK Department of Biology, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Techniques, Laboratory of Microbial Biotechnology, PB 2202, Fez, Morocco.
  • ABDELHAKIM EL OUALI LALAMI El Ghassani Hospital, Regional Health Directorate, Institute of Nursing Professions and Health Techniques Fez (Annex Meknès), 30000 Fez, Morocco.
  • KHADIJA BEKHTI Department of Biology, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Techniques, Laboratory of Microbial Biotechnology, PB 2202, Fez, Morocco.


Objective: The present study aims the investigation of the antimicrobial potential of medicinal plants selected in the central north of Morocco against methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis strain often involved in dermatitis.

Methods: Structured interviews were carried out among 91 herbalists and traditional healers through a specific information questionnaire, the in vitro susceptibility of Staphylococcus strains award ethanol extracts was evaluated using the well-diffusion assay, while the agar-microdilution method was used to determinate the minimal inhibitory concentrations (MIC). The total phenolic and flavonoids contents of all tested extracts were also determined.

Results: Based on the ethnobotanical survey, a total of 55 plant species belonging to 30 families were mentioned. The Lamiaceae family was the most represented (18.80%) followed by the Apiaceae family (10.90%). Leaves (45.00%) were the favored used part. Decoction method (48.53%) was the most frequently used to prepare remedies that are taken externally (75.00%). Nine of the 17 most selected species have shown an effective antistaphylococcal activity; the most active extracts were Punica granatum and Rhamnus alaternus with MIC values ranging between 0.25 mg/ml and 2.00 mg/ml.

Conclusion: The current data confirm the good antistaphylococcal activity of P. granatum and R. alaternus and suggest that these species could constitute a promoter source for antistaphylococcal drugs with deeply studies.

Keywords: Ethnobotanical study, Morocco, Plant extracts, Antistaphylococcal activity, Phytochemical assay.

Author Biographies

IKRAME ZEOUK, Department of Biology, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Techniques, Laboratory of Microbial Biotechnology, PB 2202, Fez, Morocco.

Departement of Biology, Laboratory of Microbial Biotechnology.

KHADIJA BEKHTI, Department of Biology, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Techniques, Laboratory of Microbial Biotechnology, PB 2202, Fez, Morocco.

Departement of Biology,  Laboratory of Microbial Biotechnology,


1. Clarke SR, Foster SJ. Surface adhesins of Staphylococcus aureus. Adv Microb Physiol 2006;51:187-224.
2. Jarvis AW, Lawrence RC. Production of extracellular enzymes and enterotoxins A, B, and C by Staphylococcus aureus. Infect Immun 1971;4:110-5.
3. Gillet Y, Issartel B, Vanhems P, Fournet JC, Lina G, Bes M, et al. Association between Staphylococcus aureus strains carrying gene for panton-valentine leukocidin and highly lethal necrotising pneumonia in young immunocompetent patients. Lancet 2002;359:753-9.
4. Valle J, Vadillo S, Piriz S, Gomez-Lucia E. Toxic shock syndrome toxin 1 (TSST-1) production by staphylococci isolated from goats and presence of specific antibodies to TSST-1 in serum and milk. Appl Environ Microbiol 1991;57:889-91.
5. Tong SY, Davis JS, Eichenberger E, Holland TL, Fowler VG Jr. Staphylococcus aureus infections: Epidemiology, pathophysiology, clinical manifestations, and management. Clin Microbiol Rev 2015;28:603-61.
6. Totté JEE, van Doorn MB, Pasmans SGMA. Successful treatment of chronic Staphylococcus aureus-Related dermatoses with the topical endolysin staphefekt SA.100: A Report of 3 cases. Case Rep Dermatol 2017;9:19-25.
7. Périchon B, Courvalin P. VanA-type vancomycin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 2009;53:4580 7.
8. Isha J, Ashwini H, Pooja R, Radhakrishna M. Nasal colonization of Methicillin-resistant Staphylococcus aureus among clinical postgraduates in a coastal Karnataka medical college prevalence and antibiogram pattern. Asian J Pharm Clin Res 2018;11:104-9.
9. Moran GJ, Krishnadasan A, Gorwitz RJ, Fosheim GE, McDougal LK, Carey RB, et al. Methicillin-resistant S. Aureus infections among patients in the emergency department. N Engl J Med 2006;355:666-74.
10. Newman DJ, Cragg GM. Natural products as sources of new drugs over the 30 years from 1981 to 2010. J Nat Prod 2012;75:311-35.
11. Swarnamoni D, Mukundam B, Shagufa A. Antibacterial activity of the ethanolic extract of leaves of Citrus maxima (burm.) merr. On Escherichia coli and Pseudomonas aeruginosa. Asian J Pharm Clin Res 2013;6:136-9.
12. Badduri N, Gupta NV, Gowda DV, Manohar M. Formulation and development of topical anti acne formulation of spirulina extract. Int J App Pharm 2018;10:229-33.
13. Bikash G, Biswajit D, Sujata P, Aditya B. Preliminary phytochemical screening and in vitro anti-microbial activity of ethanolic extract of Delonix regia (hook) raf. bark (family-Leguminosae). Int J Curr Pharm Res 2018;10:43-6.
14. Moroccan Ministry of the Interior Wilaya of the Region Fez-Meknes, Monograph of the Region Fez-Menkes, Thèses et Mémoires, C28613/14, Thèse No. DL 1987/489; 2016.
15. Kingdom of Morocco: Ministry of Interior. Fez-Meknes Regional Direction, Regional Statistical Directory Fez Meknes, Regional Direction; 2016.
16. Agency for the Promotion and Development of the North (APDN). Morocco: The Province of Taounat; 2018.
17. Yeo YL, ChiaYY, Lee CH, Sow HS, Yap WS: Effectiveness of maceration periods with different extraction solvents on in-vitro antimicrobial activity from fruit of Momordica charantia L. J Appl Pharm Sci 2014;4:16-23.
18. Elaloui M, Laamouri A, Ennajah A, Cerny M, Mathieu C, Vilarem G, et al. Phytoconstituents of leaf extracts of Ziziphus jujuba Mill. plants harvested in Tunisia. Ind Crops Prod 2016;83:133-9.
19. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 2016;6:71-9.
20. Bahorun T, Gressier B, Trotin F, Brunet C, Dine T, Luyckx M, et al. Oxygen species scavenging activity of phenolic extracts from hawthorn fresh plant organs and pharmaceutical preparations. Arzneimittelforschung 1996;46:1086-9.
21. Chaumont JP, Mandin D, Sanda K, Koba K, de Souza CA. Activités antimicrobiennes in vitro de cinq huiles essentielles de Lamiacees togolaises vis-à-vis de germes représentatifs de la microflore cutanée. Acta Bot Gall 2001;148:93-101.
22. Zgórka G, G?owniak K. Variation of free phenolic acids in medicinal plants belonging to the Lamiaceae family. J Pharm Biomed Anal 2001;26:79-87.
23. Btissam R, Fatima EIM, Mohamed N, Fatima EIM, Rubia L. In vitro Study of antibacterial activity of hydro-alcohol morrocan plants extracts. Pharmacogn J 2018;10:519-26.
24. Cocan I, Alexa E, Danciu C, Radulov I, Galuscan A, Obistioiu D, et al. Phytochemical screening and biological activity of Lamiaceae family plant extracts. Exp Ther Med 2018;15:1863-70.
25. Dulla O, Jahan FI. Ethnopharmacological survey on traditional medicinal plants at Kalaroa Upazila, Satkhira district, Khulna division, Bangladesh. J Intercult Ethnopharmacol 2017;6:316-25.
26. Azzi R, Rabah D, Farid L, Zohra SF, Houcine B, Nacéra B. Ethnopharmacological survey of medicinal plants used in the traditional treatment of diabetes mellitus in the North Western and South Western Algeria. J Med Plants Res 2012;6:2041-50.
27. Rupani R, Chavez A. Medicinal plants with traditional use: Ethnobotany in the Indian subcontinent. Clin Dermatol 2018;36:306-9.
28. Bouasla A, Bouasla I. Ethnobotanical survey of medicinal plants in Northeastern of Algeria. Phytomedicine 2017;36:68-81.
29. Kolasani A, Xu H, Millikan M. Evaluation of mineral content of Chinese medicinal herbs used to improve kidney function with chemometrics. Food Chem 2011;127:1465-71.
30. Al-Qura’n S. Ethnopharmacological survey of wild medicinal plants in Showbak, Jordan. J Ethnopharmacol 2009;123:45-50.
31. Aribi I, Chemat S, Hamdi-Pacha Y, Luyten W. Isolation of berberine tannate using a chromatography activity-guided fractionation from root bark of Berberis hispanica Boiss. and Reut. J Liq Chromatogr Relat Technol 2017;40:894-9.
32. Stelmakiene A, Ramanauskiene K, Petrikaite V, Jakstas V, Briedis V. Application of dry hawthorn (Crataegus oxyacantha l.) extract in natural topical formulations. Acta Pol Pharm 2016;73:955-65.
33. Burke-Colvin D, Hines M, Gan D. Skin Care Formulations. Justia Patents Number 9833642 United States; 2017.
34. Pacheco-Palencia LA, Noratto G, Hingorani L, Talcott ST, Mertens- Talcott SU. Protective effects of standardized pomegranate (Punica granatum L.) polyphenolic extract in ultraviolet-irradiated human skin fibroblasts. J Agric Food Chem 2008;56:8434-41.
35. Moorthy K, Punitha T, Vinodhini R, Sureshkumar BT, Vijayalakshmi P, Thajuddin N: Antimicrobial activity and qualitative phytochemical analysis of Punica granatum Linn. (PERICARP). J Med Plants Res 2013;7:474-9.
36. Park HM, Moon E, Kim AJ, Kim MH, Lee S, Lee JB, et al. Extract of Punica granatum inhibits skin photoaging induced by UVB irradiation. Int J Dermatol 2010;49:276-82.
37. Ammar RB, Sghaier MB, Boubaker J, Bhouri W, Naffeti A, Skandrani I, et al. Antioxidant activity and inhibition of aflatoxin B1-, nifuroxazide-, and sodium azide-induced mutagenicity by extracts from Rhamnus alaternus L. Chem Biol Interact 2008;174:1-0.
38. Rhimi W, Salem IB, Immediato D, Saidi M, Boulila A, Cafarchia C, et al. Chemical composition, antibacterial and antifungal activities of crude Dittrichia viscosa (L.) greuter leaf extracts. Molecules 2017;22:E942.
39. Sudjana AN, D’Orazio C, Ryan V, Rasool N, Ng J, Islam N, et al. Antimicrobial activity of commercial Olea europaea (olive) leaf extract. Int J Antimicrob Agents 2009;33:461-3.
40. Leeja L, Thoppil JE. Antimicrobial activity of methanol extract of Origanum majorana L. (Sweet marjoram). J Environ Biol 2007;28:145-6.
41. Abachi S, Khademi F, Fatemi H, Malekzadeh F. Study of antimicrobial activity of selected Iranian plant extracts on vancomycin resistant Staphylococcus epidermidis. J Dent Med Sci 2013;4:59-63.
42. Yashphe J, Segal R, Breuer A, Erdreich-Naftali G. Antibacterial activity of artemisia herba-alba. J Pharm Sci 1979;68:924-5.
43. Dlova NC, Ollengo MA. Traditional and ethnobotanic dermatology practices in Africa. Clin Dermatol 2018;36:353-63.
44. Draelos ZD. Updates in medical skin care. Adv Cosmet Surg 2018;1:211-7.
45. Cushnie TP, Lamb AJ. Recent advances in understanding the antibacterial properties of flavonoids. Int J Antimicrob Agents 2011;38:99-107.
46. Cushnie TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrob Agents 2005;26:343-56.
47. Cushnie TP, Hamilton VE, Chapman DG, Taylor PW, Lamb AJ. Aggregation of Staphylococcus aureus following treatment with the antibacterial flavonol galangin. J Appl Microbiol 2007;103:1562-7.
48. Musumeci R, Speciale A, Costanzo R, Annino A, Ragusa S, Rapisarda A, et al. Berberis aetnensis C. Presl. Extracts: Antimicrobial properties and interaction with ciprofloxacin. Int J Antimicrob Agents 2003;22:48-53.
49. Stapleton PD, Shah S, Ehlert K, Hara Y, Taylor PW. The beta-lactam-resistance modifier (-)-epicatechin gallate alters the architecture of the cell wall of Staphylococcus aureus. Microbiology 2007;153:2093-103.
50. Orhan DD, Hartevio?lu A, Küpeli E, Yesilada E. In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. Fruits. J Ethnopharmacol 2007;112:394-400.
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