• IKRAME ZEOUK Department of Biology, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Techniques, Laboratory of Microbial Biotechnology, PB 2202, Fez, Morocco.
  • ABDELHAKIM EL OUALI LALAMI El Ghassani Hospital, Regional Health Directorate, Institute of Nursing Professions and Health Techniques Fez (Annex Meknès), 30000 Fez, Morocco.
  • KHADIJA BEKHTI Department of Biology, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Techniques, Laboratory of Microbial Biotechnology, PB 2202, Fez, Morocco.


Objective: The present study aims the investigation of the antimicrobial potential of medicinal plants selected in the central north of Morocco against methicillin-resistant Staphylococcus aureus and Staphylococcus epidermidis strain often involved in dermatitis.

Methods: Structured interviews were carried out among 91 herbalists and traditional healers through a specific information questionnaire, the in vitro susceptibility of Staphylococcus strains award ethanol extracts was evaluated using the well-diffusion assay, while the agar-microdilution method was used to determinate the minimal inhibitory concentrations (MIC). The total phenolic and flavonoids contents of all tested extracts were also determined.

Results: Based on the ethnobotanical survey, a total of 55 plant species belonging to 30 families were mentioned. The Lamiaceae family was the most represented (18.80%) followed by the Apiaceae family (10.90%). Leaves (45.00%) were the favored used part. Decoction method (48.53%) was the most frequently used to prepare remedies that are taken externally (75.00%). Nine of the 17 most selected species have shown an effective antistaphylococcal activity; the most active extracts were Punica granatum and Rhamnus alaternus with MIC values ranging between 0.25 mg/ml and 2.00 mg/ml.

Conclusion: The current data confirm the good antistaphylococcal activity of P. granatum and R. alaternus and suggest that these species could constitute a promoter source for antistaphylococcal drugs with deeply studies.

Keywords: Ethnobotanical study, Morocco, Plant extracts, Antistaphylococcal activity, Phytochemical assay.

Author Biographies

IKRAME ZEOUK, Department of Biology, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Techniques, Laboratory of Microbial Biotechnology, PB 2202, Fez, Morocco.

Departement of Biology, Laboratory of Microbial Biotechnology.

KHADIJA BEKHTI, Department of Biology, Sidi Mohamed Ben Abdellah University, Faculty of Sciences and Techniques, Laboratory of Microbial Biotechnology, PB 2202, Fez, Morocco.

Departement of Biology,  Laboratory of Microbial Biotechnology,


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