QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

  • Ruben Ashotovich Pogosyan Department of Chemistry, Sechenov First Moscow State Medical University, Russia.
  • Olga Vladimirovna Nesterova Department of Chemistry, Sechenov First Moscow State Medical University, Russia.
  • Dmitry Olegovich Bokov Department of Pharmaceutical and Natural Sciences, Sechenov First Moscow State Medical University, Russia. http://orcid.org/0000-0003-2968-2466
  • Irina Alexandrovna Samylina Department of Pharmaceutical and Natural Sciences, Sechenov First Moscow State Medical University, Russia.

Abstract

Objective: Pomegranate (Punica granatum L.) is a broadly used plant possessing a wide range of medicinal properties. In this research, we have mainly focused on the investigation of phenolic compounds of pomegranate fruit pulp (PFP).

Methods: Fresh fruits of “Çəhrayı Gülöyşə,†“Kizil-anor,†and pomegranate varietal mixture were used as samples. High-performance liquid chromatography-ultraviolet (HPLC-UV) analysis of phenol carboxylic acids was performed with metal column Kromasil® C18 (4.6×250 mm, particle size 5 μm) and the acetonitrile-water-concentrated acid phosphoric system (400:600:5) under isocratic elution conditions (flow rate of 0.5 ml/min). Detection was carried out using a UV detector “GILSTON†UV/Visible model 151 at a wavelength of 280 nm.

Results and Discussion: As a result of our research, we proposed chromatographic conditions for the separation of phenolic compounds, the conditions for sample preparation of PFP. Procedure for determination of phenolic carboxylic acids total content in terms of gallic acid by HPLC-UV method was developed. According to the obtained data, the content of phenolic carboxylic acids should be at least 0.7%.

Conclusion: Procedure for the quantitative determination of gallic acid using the HPLC-UV method was developed. This method which can be used in the standardization of new medicinal plant raw materials - PFP, as well as extract preparations based on it in the future.

Keywords: Pomegranate fruit pulp, Punica granatum L., Phenol carboxylic acids, Gallic acid, high-performance liquid chromatography with ultraviolet detection.

Author Biography

Dmitry Olegovich Bokov, Department of Pharmaceutical and Natural Sciences, Sechenov First Moscow State Medical University, Russia.
assistant

References

1. Gruzdev VF. Russian Handwritten Medical Books. Leningrad: Military-medical Academy Publishing House; 1946. p. 72.
2. Toren MD. The use of medicinal plants in Russian traditional medicine. 7th The International Congress of Anthropologists and Ethnographic Sciences. Moscow; 1964. p. 56.
3. Aulus Cornelius Celsus. About Medicine. Works of 25-30 Years AD. Translation from Latin. Moscow; 1959.
4. Aseeva TA, Bolkhosoeva ND, Bukhasheva TG, Dashiev DB. Atlas of Tibetan Medicine. A Collection of Illustrations for the Tibetan Medical Treatise of the 17th Century “Blue Beryl”. Moscow: Galart Publishing House; 1994. p. 592.
5. Ministry of Public Health of the Russian Federation. Appendix No. 2 to Order No. “Nomenclature of Single-Component Homeopathic Medicines Authorized for Medical use on the Territory of the Russian Federation”; 28 December, 1995.
6. Kirilenko OA. Antibacterial Properties of Juice from Different Varieties Pogranate. Moscow: Canning and Vegetable Drying Industry; 1978. p. 12-3.
7. Zykina TF, Kostinskaya LI. Polyphenolic compounds of pomegranate, Izvestia Vuzov. Food Technol Krasnodar 1984;3:117-9.
8. Karasharly AS. Processing of pomegranate without waste. Gardening, Moscow. Kolos 1981;1:153-5.
9. Sreedevi P, Vijayalakshmi K, Venkateswari R. Phytochemical evaluation of Punica granatum L. leaf extract. Int J Curr Pharm Res 2017;9:14-8.
10. Rylina EV. Determination of indicator phenolic compounds of nonflavonoid nature in medicinal and edible plant raw materials by the HPLC method. Candidate of Pharmaceutical Sciences Thesis. Moscow; 2010. p. 112.
11. Andreotti C, Costa G, Treutter D. Composition of phenolic compounds in pear leaves as affected by genetics, ontogenesis and the environment. Sci Hortic 2006;109:130-7.
12. Derakhshan Z, Ferrante M, Tadi M, Ansari F, Heydari A, Hosseini MS, et al. Antioxidant activity and total phenolic content of ethanolic extract of pomegranate peels, juice and seeds. Food Chem Toxicol 2018;114:108-11.
13. Bassiri-Jahromi S. Punica granatum (Pomegranate) activity in health promotion and cancer prevention. Oncol Rev 2018;12:345.
14. Hamza RZ, Abdel-Azez AM, Hussien NA. Evaluation of the antioxidant potential for different extracts of Al-Taif pomegranate (Punica granatum L.) induced by atrazine and malathion pesticides in liver of male albino mice. Int J Pharm Sci 2015;7:89-94.
15. Taha O, Barakat A, Abdelhakim H, Shemis M, Zakaria Z. Hypolipidemic and anti-fatty liver effects exerted by standardized Punica granatum L. peel extract in hepg2 cell-line and high-fat diet-induced mice. Int J Pharm Pharm Sci 2016;8:156-61.
16. Aditya JB, Biswajit D, Sujata P, Bikash G. Preliminary phytochemical screening and in vitro anti-microbial activity of ethanolic extracts of seeds of Punica granatum against standard pathogenic strains. Int J Curr Pharm Res 2018;10:55-8.
17. Skurkhin IM, Tutelyan VA. Guide for Analyzing Methods of Food Quality and Safety. Moscow: Medicine; 1998. p. 188.
18. Styskin EL, Itzikson LB, Braude EV. Practical High-Performance Liquid Chromatography. Moscow: Chemistry; 1986. p. 312.
19. Wagner H, Bladt S, Zgainski EM. Plant Drug Analysis. Berlin, Germany: Springer; 1984.
20. Shatz VD, Sahartova OV. Highly Effective Liquid Chromatography: Fundamentals of Theory. Methodology. Application in Drug Chemistry. Riga: Zinatne; 1988. p. 380.
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How to Cite
Pogosyan, R. A., O. V. Nesterova, D. O. Bokov, and I. A. Samylina. “QUANTITATIVE DETERMINATION OF PHENOL CARBOXYLIC ACIDS IN POMEGRANATE FRUIT PULP BY THE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 1, Jan. 2019, pp. 548-50, doi:10.22159/ajpcr.2019.v12i1.30589.
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