PHYTOCHEMICAL, GAS CHROMATOGRAPHY WITH MASS SPECTROMETRY ANALYSIS OF ANDROGRAPHIS SERPYLLIFOLIA METHANOL LEAF EXTRACT AND ITS ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES

  • MADHANKUMAR R Department of Botany, School of Life Sciences, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, India.
  • MURUGESAN S Department of Botany, School of Life Sciences, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, India.

Abstract

Objective: The present study is to evaluate the preliminary study of phytochemical screening and biological applications of Andrographis serpyllifolia methanol leaf extracts.


Methods: The methanol leaf extracts of A. serpyllifolia was prepared using Soxhlet apparatus and the extract was analyzed using gas chromatography with mass spectrometry (GC-MS). In vitro antioxidant activity was determined by superoxide dismutase, catalase, glutathione peroxidase, and glutathione S-transferase. Further, the antibacterial activity of methanolic leaf extract of A. serpyllifolia was tested against various human pathogens by using agar disc diffusion method.


Results: Preliminary phytochemical screening and GC-MS results revealed phenols, aromatic carboxylic acids, and esters in the chloroform extract to be the molecules responsible for the antioxidant and antibacterial activity of A. serpyllifolia methanol extract and fractions showed the presence of various secondary metabolites present.


Conclusion: The present study strongly recommended that the methanolic extract of A. serpyllifolia leaves possesses compounds that inhibit the growth of microbes as wells excellent antioxidant activities. The study further suggested the potential therapeutic use of these extract in cancer study.

Keywords: A. serpyllifolia, Methanol extracts, Phytochemical, Gas chromatography with mass spectrometry, Antimicrobial, Antioxidant.

Author Biography

MADHANKUMAR R, Department of Botany, School of Life Sciences, Periyar University, Periyar Palkalai Nagar, Salem, Tamil Nadu, India.

Assistant professor

References

1. Prakash P, Gupta N. Therapeutic uses of Ocimum sanctum Linn (Tulsi) with a note on eugenol and its pharmacological actions: A short review. Indian J Physiol Pharmacol 2005;49:125-31.
2. Mahesh B, Satish S. Antimicrobial activity of some important medicinal plant against plant and human pathogens. World J Agric Sci 2008;4:839-43.
3. Banu KS, Cathrine L. General techniques involved in phytochemical analysis. Int J Adv Res Chem Sci 2015;2:25-32.
4. Muhammad H, Gomes-Carneiro MR, Poça KS, De-Oliveira AC, Afzan A, Sulaiman SA, et al. Evaluation of the genotoxicity of Orthosiphon stamineus aqueous extract. J Ethnopharmacol 2011;133:647-53.
5. Chithra S, Murthy NK, Srinivas C. Phytochemical screening and in vitro assessment of antimicrobial and antioxidant potential of A. serpyllifolia-an endemic medicinal plant from South India. Int J Adv Res 2014;2:917-28.
6. Sanjeevaiah N, Jithan A. Pharmacological screening of A. serpyllifolia for antidiabetic activity. Int J Adv Pharma 2013;2:2277-4688.
7. Madav S, Tripathi HC, Mishra SK. Analgesic, antipyretic and antiulcerogenic effects of andrographolide. Ind J Pharm Sci 1994;57:121-5.
8. El-Shouny WA, Ali SS, Sun J, Samy SM, Ali A. Drug resistance profile and molecular characterization of extended spectrum beta-lactamase (ES?L)-producing Pseudomonas aeruginosa isolated from burn wound infections. Essential oils and their potential for utilization. Microb Pathog 2018;116:301-12.
9. Ahmed SA, Kaur G, Sharma P, Singh S, Ikram S. Fruit waste (peel) as bio-reductant to synthesize silver nanoparticles with antimicrobial, antioxidant and cytotoxic activities. J Appl Biomed 2018;16:221-31.
10. Kumar MR, Joshi SD, Kulkarni VH, Savant C. Phytochemical screening and evaluation of analgesic, anti-inflammatory activities of Peganum harmala Linn., Seeds in rodents. Int J Appl Pharm Sci 2015;5:52-5.
11. Nayak D, Ashe S, Rauta PR, Nayak BB. Assessment of antioxidant, antimicrobial and anti-osteosarcoma potential of four traditionally used Indian medicinal plants. J Appl Biomed 2017;15:119-32.
12. Gandhia PR, Jayaseelan C, Kamaraj C, Rajasree SR, Mary RR. In vitro antimalarial activity of synthesized TiO2 nanoparticles using Momordica charantia leaf extract against Plasmodium falciparum. J Appl Biomed 2018;18:221-31.
13. Tahaa H, Aryab A, Khan AK, Shahid N, Noordind MI, Mohan S. Effect of Pseuduvaria macrophylla in attenuating hyperglycemia mediated oxidative stress and inflammatory response in STZ-nicotinamide induced diabetic rats by upregulating insulin secretion and glucose transporter-1, 2 and 4 proteins expression. J Appl Biomed 2018;16:263 73.
14. Ali SS, Morsy R, El-Zawawy NA, Fareed M, Bedaiwy MY. Synthesized zinc peroxide nanoparticles (ZnO2-NPs): A novel antimicrobial, anti-elastase, antikeratinase, and anti-inflammatory approach toward polymicrobial burn wounds. Int J Nanomed Nanosurg 2017;12:6059 73.
15. Bloemsma GC, Dokter J, Boxma H, Oen IM. Mortality and causes of death in a burn centre. Burns 2008;34:1103-7.
16. Kandati PV, Ch G, Reddy AS, Nath RR, Reddy R. In-vitro and in-vivo antiinflammatory activity of Andrographis serpyllifolia (Rottl. Ex Vahl.) Wt. Int Curr Pharm J 2012;1:199-204.
17. Harborne AJ. Phytochemical methods a guide to modern techniques of plant analysis. Plant Pathol 1998;48:146.
18. Sinha AK. Colorimetric assay of catalase. Anal Biochem 1972;47:389 94.
19. Rotruck JT, Pope AL, Ganther HE, Swanson AB, Hafeman DG, Hoekstra WG, et al. Selenium: Biochemical role as a component of glutathione peroxidase. Science 1973;179:588-90.
20. Habig WH, Pabst MJ, Jacoby WB. Glutathione-S-transferase the first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130-9.
21. Rawat P, Bachheti R, Kumar N, Rai N. Phytochemical analysis and evaluation of in vitro immunomodulatory activity of Rhododendron arboreum leaves. Asian J Pharm Clin Res 2018;11:123-8.
22. Roy JD, Handique AK, Barua CC, Talukdar A, Ahmed FA, Barua IC. Evaluation of phytoconstituents and assessment of adaptogenic activity in vivo in various extracts of Rhododendron arboreum (leaves). Ind J Pharm Biol Res 2014;2:49-56.
23. Osama A, Awadelkarim S, Ali A. Antioxidant activity, acetylcholinesterase inhibitory potential and phytochemical analysis of Sarcocephalus latifolius Sm. Bark used in traditional medicine in Sudan. BMC Complement Altern Med 2017;17:270.
24. Chen JJ, Ting CW, Hwang TL, Chen IS. Benzophenone derivatives from the fruits of Garcinia multiflora and their anti-inflammatory activity. J Nat Prod 2009;72:253-8.
25. Bowler C, Montagu MV, Inze D. Superoxide dismutase and stress tolerance. Ann Rev Plant Physiol Plant Mol Biol 1992;43:83-116.
26. Renuka K, Devi VR, Subramanian SP. Phytochemical screening and evaluation of in vitro antioxidant potential of immature palmyra palm (Borassus flabellifer Linn.) fruits. Int J Pharm Pahrm Sci 2018;9:30-4.
27. Ahmed A, Hasnain A, Akhtar S, Hussain A, Yasin AU, Wahid A, et al. Antioxidant enzymes as bio-markers for copper tolerance in safflower (Carthamus tinctorius L.). Afr J Biotechnol 2010;9:5441-4.
28. Starlin T, Gopalakrishnan VK. Enzymatic and non-enzymatic antioxidant properties of Tylophora pauciflora Wight and arn.-an in vitro study. Asian J Pharm Clin Res 2013;6:68-71.
29. Azalework HG, Sahabjada, Jafri A, Arshad MD, Malik T. Phytochemical investigation, GC-MS profile and antimicrobial activity of a medicinal plant Ruta graveolens. From Ethiopia. Int J Pharm Pharm Sci 2017;9:30-4.
30. Gupta SS, Sharma J, Kumar GR, Pandey G, Mohapatra PK, Rawat AS, et al. Effect of A. serpyllifolia Leaves extract on experimentally induced typhoid using Salmonella typhi. Br J Pharm Res 2014;3:230-9.
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R, M., and M. S. “PHYTOCHEMICAL, GAS CHROMATOGRAPHY WITH MASS SPECTROMETRY ANALYSIS OF ANDROGRAPHIS SERPYLLIFOLIA METHANOL LEAF EXTRACT AND ITS ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 343-7, https://innovareacademics.in/journals/index.php/ajpcr/article/view/30618.
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