INVESTIGATION OF ANTIOXIDANT ACTIVITY (IN VITRO) AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING OF PORTULACA OLERACEA L. AND PORTULACA GRANDIFLORA HOOK. EXTRACTS

  • KINICHENKO AO Department of Pharmacognosy, Pharmacology and Botany, Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine.

Abstract

Objective: The aim of this research was to investigate the antioxidant activity (AOA) of the methanolic extracts of different parts of Portulaca oleracea and Portulaca grandiflora.


Methods: The plant different parts were extracted with methanol. The methanolic extracts of both species were screened for 2,2-diphenyl-1- picrylhydrazyl radical scavenging abilities and researched by gas chromatography-mass spectrometry (GC-MS) analysis.


Results: GC-MS analysis of P. oleracea root extract revealed the presence of 39 biologically active compounds. 32 biologically active compounds were identified in P. grandiflora root extract. Both extracts revealed the AOA.


Conclusion: The results of the investigation could be useful for further pharmacological and phytochemical researches to assess the beneficial properties of both these species.

Keywords: Portulaca oleracea L., Portulaca grandiflora Hook., 2,2-Diphenyl-1-picrylhydrazyl method, Gas chromatography-mass spectrometry analysis.

References

1. Hrodzynskyi AM. Medicinal Plants: Encyclopedic Guide. Kyiv: Ukrainian Encyclopedia MP Bazhana; 1990.
2. Wasnik DD, Tumane PM. Preliminary phytochemical screening and evaluation of antibacterial activity of Portulaca oleracea. World J Pharm Res 2014;3:920-32.
3. Azuka OI, Mary BA. A review on Portulaca oleracea (Purslane) plant its nature and biomedical benefits. J Biomed Mater Res 2014;5:75-80.
4. Zhao R, Zhang T, Ma B, Li X. Antitumor activity of Portulaca oleracea L. Polysaccharide on heLa cells through inducing TLR4/NF- ?B signaling. Nutr Cancer 2017;69:131-9.
5. Gu JF, Zheng ZY, Yuan JR, Zhao BJ, Wang CF, Zhang L, et al. Comparison on hypoglycemic and antioxidant activities of the fresh and dried Portulaca oleracea L. In insulin-resistant hepG2 cells and streptozotocin-induced C57BL/6J diabetic mice. J Ethnopharmacol 2015;161:214-23.
6. Al-Sheddi ES, Farshori NN, Al-Oqail MM, Musarrat J, Al-Khedhairy AA, Siddiqui MA, et al. Portulaca oleracea seed oil exerts cytotoxic effects on human liver cancer (HepG2) and human lung cancer (A-549) cell lines. Asian Pac J Cancer Prev 2015;16:3383-7.
7. Ashok KJ, Mudasir B. Efficient micropropagtion protocol for Portulaca grandiflora Hook. Using shoot tip explants. N Y Sci J 2010;3:112-6.
8. Kazunin MS, Voskoboynik OY, Nosulenko IS, Berest GG, Sergeieva T, Okovytyy S, et al. Synthesis, tautomerism, and antiradical activity of novel pteridinetrione derivatives. J Heterocycl Chem 2018;5:1033-41.
9. Sreedevi P, Vijayalakshmi K. Determination of antioxidant capacity and gallic acid content in ethanolic extract of Punica granatum L. Leaf. Asian J Pharm Clin Res 2018;11:319-23.
10. Hasan HT, Kadhim EJ. Phytochemical investigation of leaves and seeds of Corchorus olitorius L. cultivated in Iraq. Asian J Pharm Clin Res 2018;11:408-17.
11. Mousavi SR, Niazmand R, Noghabi MS. Antioxidant activity of purslane (Portulaca oleracea L.) seed hydro-alcoholic extract on the stability of soybean oil. J Agric Sci Tech 2015;17:1473-80.
12. Erkan N. Antioxidant activity and phenolic compounds of fraction from Portulaca oleracea L. Food Chem 2012;133:775-81.
13. Dkhil MA, Moniem AE, Al-Quraishy S, Saleh RA. Antioxidant effect of purslane (Portulaca oleracea) and its mechanism of action. J Med Plant Res 2011;5:1563-89.
14. Uddin MK, Juraimi AS, Ali ME, Ismail MR. Evaluation of antioxidant properties and mineral composition of purslane (Portulaca oleracea L.) at different growth stages. Int J Mol Sci 2012;13:10257-67.
15. Li Y, Hu Y, Shi S, Jiang L. Evaluation of antioxidant and immuno-enhancing activities of purslane polysaccharides in gastric cancer rats. Int J Biol Macromol 2014;68:113-6.
Statistics
51 Views | 117 Downloads
How to Cite
AO, K. “INVESTIGATION OF ANTIOXIDANT ACTIVITY (IN VITRO) AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY PROFILING OF PORTULACA OLERACEA L. AND PORTULACA GRANDIFLORA HOOK. EXTRACTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 348-52, https://innovareacademics.in/journals/index.php/ajpcr/article/view/30621.
Section
Original Article(s)