• AYESHA HUSNA S Department of Pharmacology, Krishna Teja Pharmacy College, Tirupati, Andhra Pradesh, India,
  • JAYASANKAR REDDY V Department of Pharmacology, Krishna Teja Pharmacy College, Tirupati, Andhra Pradesh, India,


Objective: The objective of this study is to evaluate the antipsoriatic activity of aqueous extract of Brassica oleracea var. capitata leaves (BOAE) and ethanolic extract of Mentha spicata leaves (MSEE) on Imiquimod-induced Psoriasis-like dermatitis.

Methods: Imiquimod induction method for psoriasis-like dermatitis was used for the evaluation of antipsoriatic activity. Extracts were tested at a dose of 100 mg/kg body weight (b.wt.) and 400 mg/kg b.wt. in Swiss albino mice. These test doses were compared with the Retino-A (0.025%) treated group which serves as a standard group. Parameters studied in the test include changes in body weight, spleen weight and spleen index, neutrophils, lymphocytes, eosinophils, monocytes, hemoglobin, red blood cells, platelets, packed cell volume, mean corpuscular volume, mean corpuscular hemoglobin (MCH), MCH concentration, epidermal thickness, erythema, desquamation, skin thickness, superoxide dismutase, catalase, lipid peroxidation of skin and percentage orthokeratotic values, drug activity, and relative epidermal thickness.

Results: MSEE at dose of 400 mg/kg b.wt has shown more effect when compared with MSEE at low dose, BOAE topical and oral.

Conclusion: From the study, it was concluded that the above extracts have promising anti-psoriatic activity.

Keywords: Brassica oleracea var. capitata, Mentha spicata, Psoriasis, Imiquimod, Retino-A (0.025%).


1. Azfar RS, Gelfand JM. Psoriasis and metabolic disease: Epidemiology and pathophysiology. Curr Opin Rheumatol 2008;20:416-22.
2. Bowcock AM, Cookson WO. The genetics of psoriasis, psoriatic arthritis and atopic dermatitis. Hum Mol Genet 2004;13:R43-55.
3. Amigó M, Payá M, De Rosa S, Terencio MC. Antipsoriatic effects of avarol-3’-thiosalicylate are mediated by inhibition of TNF-alpha generation and NF-kappaB activation in mouse skin. Br J Pharmacol 2007;152:353-65.
4. Mandloi S, Mishra R, Yadav N, Yadav S. Antibacterial activity of Mentha spicata ethanol leaf extract against Pseudomonas aeruginosa isolated from upper respiratory tract of T.B. negative patients. World J Pharm Pharm Sci 2016;6:837-47.
5. Lewis JJ. Cabbage extracts and insulin-like activity. Br J Pharmacol Chemother 1950;5:21-4.
6. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. Vol. 40. Pune: Nirali Prakashan; 2007. p. 1-635.
7. Nadeem A, Ahmad SF, Al-Harbi NO, El-Sherbeeny AM, Al-Harbi MM, Almukhlafi TS, et al. GPR43 activation enhances psoriasis-like inflammation through epidermal upregulation of IL-6 and dual oxidase 2 signaling in a murine model. Cell Signal 2017;33:59-68.
8. Swindell WR, Michaels KA, Sutter AJ, Diaconu D, Fritz Y, Xing X, et al. Imiquimoid has strain-dependent effects in mice and does not uniquely model human psoriasis. Genome Med 2017;9:1-21.
9. Nakaguma H, Kambara T, Yamamoto T. Rat ultraviolet ray B photodermatitis: An experimental model of psoriasis vulgaris. Int J Exp Pathol 1995;76:65-73.
10. Singhal M, Kansara N. Cassia tora Linn cream inhibits ultraviolet-B-induced psoriasis in rats. ISRN Dermatol 2012;2012:346510.
11. Grine L, Steeland S, Van Ryckeghem S, Ballegeer M, Lienenklaus S, Weiss S, et al. Topical imiquimod yields systemic effects due to unintended oral uptake. Sci Rep 2016;6:1-6.
12. Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J 2013;21:143 52.
13. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
14. Buege J, Aust SD. Microsomal lipid peroxidation. In: Colowick SP, Kaplan NO, editors. Methods in Enzymology. New York: Academic Press; 1978. p. 302-11.
15. van der Fits L, Mourits S, Voerman JS, Kant M, Boon L, Laman JD, et al. Imiquimod-induced psoriasis-like skin inflammation in mice is mediated via the IL-23/IL-17 axis. J Immunol 2009;182:5836-45.
16. Psoriasis Revolution. Psoriasis Cure-How to Cure Psoriasis 100% Naturally; 2017. Available from: https://www.youtube.com.
17. Ashley Marcin. Can You Use Oils to Treat Psoriasis? 2018. Available from: https://www.healthline.com/health/essential-oils-for-psoriasis.
18. Al-Mosawi AM. Antipsoriatic activity of oral and topical milk thistle extract against imiquimod-induced psoriasis-like skin lesions in balb/c mice. J Int Acad Res Multidiscip 2016;4:1-10.
19. Na Takuathung M, Wongnoppavich A, Panthong A, Khonsung P, Chiranthanut N, Soonthornchareonnon N, et al. Antipsoriatic effects of wannachawee recipe on imiquimod-induced psoriasis-like dermatitis in BALB/c mice. Evid 2018;2018:7931031.
20. Hu SC, Yu HS, Yen FL, Lin CL, Chen GS, Lan CC. Neutrophil extracellular trap formation is increased in psoriasis and induces human ?-defensin-2 production in epidermal keratinocytes. Sci Rep 2016;6:3119.
21. Langewouters AM, van Erp PE, de Jong EM, van de Kerkhof PC. Lymphocyte subsets in peripheral blood of patients with moderate-to-severe versus mild plaque psoriasis. Arch Dermatol Res 2008;300:107 13.
22. Kim HJ, Roh JY, Jung Y. Eosinophils accelerate pathogenesis of psoriasis by supporting an inflammatory milieu that promotes neutrophil infiltration. J Invest Dermatol 2018;138:2185-94.
23. Golden JB, Groft SG, Squeri MV, Debanne SM, Ward NL, McCormick TS, et al. Chronic psoriatic skin inflammation leads to increased monocyte adhesion and aggregation. J Immunol 2015;195:2006-18.
24. Berentsen S, Sundic T. Red blood cell destruction in autoimmune hemolytic anemia: Role of complement and potential new targets for therapy. J Biomed Biotechnol 2015;363278:1-11.
25. Harry S. Jacob. Enlarged spleen (Splenomegaly); 2018. Available from: https://www.msdmanuals.com>home>blood disorders>spleen disorders.
26. Ponikowska M, Tupikowska M, Kasztura M, Jankowska EA, Szepietowski JC. Deranged iron status in psoriasis: The impact of low body mass. J Cachexia Sarcopenia Muscle 2015;6:358-64.
27. Bosman B, Matthiesen T, Hess V, Friderichs E. A quantitative method for measuring antipsoriatic activity of drugs by the mouse tail test. Skin Pharmacol 1992;5:41-8.
28. Ighodaro OM, Akinloye OA. First line defence antioxidants-superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX): Their fundamental role in the in the entire antioxidant defence grid. Alexandria Med J 2017;2017:1-7.
29. Ferretti G, Bacchetti T, Campanati A, Simonetti O, Liberati G, Offidani A, et al. Correlation between lipoprotein(a) and lipid peroxidation in psoriasis: Role of the enzyme paraoxonase-1. Br J Dermatol 2012;166:204-7.
30. Vijayalakshmi A, Geetha M. Anti-psoriatic activity of flavonoids from Cassia tora Leaves using the rat ultraviolet B ray photodermatitis model. Rev Bras Farmacogn 2014;24:322-9.
31. Shrivastav S, Sindhu RK, Kumar S, Kumar P. Anti-psoriatic and phytochemical evaluation of Thespesia populnea bark extracts. Int J Pharm Pharm Sci 2009;1:176-85.
32. Muruganantham N, Basavara KH, Dhanabal SP, Praveen T, Shamasundar NM, Rao KS. Screening of Caesalpinia bonduc leaves for antipsoriatic activity. J Ethnopharmacol 2011;133:897-91.
33. Ahmed MF, Rao AS, Ahmed SR, Ibrahim M. Phytochemical studies and antioxidant activities of Brassica oleraceae. L. var.Capitata. Int J Pharm Pharm Sci 2012;4:374-8.
34. Barbara EO. Psoriasis it is not. J Clin Rheumatol 2013;19:296.
35. Abdelgawad R, Nasr M, Hamza MY, Awad GA. Topical and systemic dermal carriers for psoriasis. Int J Curr Pharm Rev Res 2016;8:4-9.
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How to Cite
HUSNA S, A., and J. R. V. “EVALUATION OF ANTIPSORIATIC ACTIVITY OF AQUEOUS EXTRACT OF BRASSICA OLERACEA VAR. CAPITATA AND ETHANOLIC EXTRACT OF MENTHA SPICATA LEAVES ON IMIQUIMOD-INDUCED PSORIASIS-LIKE DERMATITIS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 359-65, https://innovareacademics.in/journals/index.php/ajpcr/article/view/30693.
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