PROTECTIVE ROLE OF AQUEOUS EXTRACT OF ATRIPLEX HALIMUS L. AGAINST BENZENE-INDUCED DAMAGE ON RENAL FUNCTION AND GLOMERULAR CELLS IN RATS
Objective: Benzene (BZ) is an important pollutant compound, present in both occupational and general environment that has been linked to adverse health effect. The aim of the current study was to investigate the curative and preventive effect of Atriplex halimus L. (Ah) extract against BZ-induced nephrotoxicity in rats.
Methods: A total of 30 male albino Wistar rats were divided into five groups (n=6): Control, Ah, BZ, AhP+BZ, and BZ+AhC. BZ (100 mg/kg b.w) was added in their drinking water for 15 weeks. Ah (Aqueous Extract of aerial parts) was given by gavage during the past 30 days of BZ-exposed for curative treatment (AhC) and all the duration of BZ exposure in the animals for preventive treatment (AhP). Some biochemical, oxidative stress parameters, and histopathology of kidney tissue were studied.
Results: Obtained results revealed that BZ exposure to rats caused a significant elevation in urea, creatinine, and malondialdehyde levels. Then, it led to reducing the glutathione level, catalase, and glutathione-S-transferase activities when compared to the control animals. Histopathological studies showed a massive damage in kidneys of the BZ-exposed rat. Our results showed that curative treatment of aqueous extract of Ah has a partial correction of the biochemical, oxidative stress parameters, and kidney morphology, but preventive treatment has a dramatic effect on the previous parameters.
Conclusion: Results demonstrated the beneficial effects of Ah preventive treatment against stress oxidative and kidney damage induced by BZ.
2. Mathews JM, Etheridge AS, Matthews HB. Highlight dose-dependent metabolism of benzene in hamsters rats and mice. J Environ Sci 1998;44:14-21.
3. Çavu?o?lu K, Yalçin E, Yapar K, Gür B, Çiçek F. The protective role of grape seed extract against chronic toxicity of benzene in swiss albino mice. Cumhuriyet Sci J 2014;35:1-11.
4. Maltoni C, Ciliberti A, Cotti G, Conti B, Belpoggi F. Benzene, an experimental multipotential carcinogen: Results of the long-term bioassays performed at the bologna institute of oncology. Environ Health Perspect 1989;82:109-24.
5. Verma Y, Rana, SVS, Singh N. Simultaneous exposure to benzene and alcohol in petrol pump operators. Int J Pharm Sci Res 2016;7:2606-10.
6. Medinsky MA, Schlosser PM, Bond JA. Critical issues in benzene toxicity and metabolism: The effect of interactions with other organic chemicals on risk assessment. Environ Health Perspect 1994;102 Suppl 9:119-24.
7. Emara AM, El-Bahrawy H. Green tea attenuates benzene-induced oxidative stress in pump workers. J Immunotoxicol 2008;5:69-80.
8. Falzone L, Marconi A, Loreto C, Franco S, Spandidos DA, Libra M, et al. Occupational exposure to carcinogens: Benzene, pesticides and fibers (Review). Mol Med Rep 2016;14:4467-74.
9. El Batsh MM, Zakaria SS, Gaballah HH. Protective effects of alpha-lipoic acid against benzene induced toxicity in experimental rats. Eur Rev Med Pharmacol Sci 2015;19:2717-24.
10. Derouiche S, Zeghib K, Gharbi S, Khelef Y, Feriani A. Protective effects of aristolochia longa and aquilaria malaccensis against lead induced acute liver injury in rats. J Acute Dis 2017;6:193-19.
11. Tucker PS, Scanlan AT, Dalbo VJ. Chronic kidney disease influences multiple systems: Describing the relationship between oxidative stress, inflammation, kidney damage, and concomitant disease. Oxid Med Cell Longev 2015;2015:806358.
12. Oh GS, Kim HJ, Shen A, Lee SB, Khadka D, Pandit A, et al. Cisplatin-induced kidney dysfunction and perspectives on improving treatment strategies. Electrolyte Blood Press 2014;12:55-65.
13. Afravy M, Angali K, Khodadadi A, Ahmadizadeh M. The protective effect of buffalo’s milk against toluene induced-nephrotoxicity in rats. J Nephropathol 2017;6:174-9.
14. Basu S, Das M, Sen A, Datta G. Protective role of crude extract of Amorphophallus Campanulatus against ethanol-induced oxidative renal damage. Asian J Pharm Clin Res 2018;11:240-50.
15. Nedjimi B, Guit B, Toumi M, Beladel B, Akam A. Daoud Y. Atriplex halimus Subsp. schweinfurthii (Chenopodiaceae): Description, écologie et utilisations pastorales et thérapeutiques. Fourrages 2013;216:333-8.
16. Kabbash AM. Macroscopic and microscopic charachterization of Atriplex halimus L. growing in egypt and in vitro evaluation of its cytotoxic activity. W J Pharm Pharm Sci 2016;5:84-100.
17. Murugan R, Parimelazhagan T. Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckia parvifolia Arn. An in vitro approach. J King Saud Univ Sci 2014;26:267-75.
18. Li HB, Cheng KW, Wong CC, Fan KW, Chen F, Jiang Y. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chem 2007;102:771-6.
19. Broadhurst RB, Jones WT. Analysis of condensed tannins using acidified vanillin. J Sci Food Agric 1978;29:788-94.
20. Snyder R, Hedli CC. An overview of benzene metabolism. Environ Health Perspect 1996;104 Suppl 6:1165-71.
21. Quintanilha AT, Packer L, Davies JM, Racanelli TL, Davies KJ. Membrane effects of vitamin E deficiency: Bioenergetic and surface charge density studies of skeletal muscle and liver mitochondria. Ann N Y Acad Sci 1982;393:32-47.
22. Weckbecker G, Cory JG. Ribonucleotide reductase activity and growth of glutathione-depleted mouse leukemia L1210 cells in vitro. Cancer Lett 1988;40:257-64.
23. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130-9.
24. Aebi H. “Catalase in vitro”. Methods Enzymol 1984;105:121-6.
25. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.
26. Ibrahim KS, Saleh ZA, Farrag AR, Shaban EE. Protective effects of zinc and selenium against benzene toxicity in rats. Toxicol Ind Health 2011;27:537-45.
27. Kadeche L, Bourogaa E, Saoudi M, Boumendjel A, Djeffal A, El Feki A, et al. Ameliorative effects of vanillin against metribuzin-induced oxidative stress and toxicity in rats. Int J Pharm Pharm Sci 2017;9:56 62.
28. Moirangthem RS, Gunindro N, Takhellambam DS, Khuraijam SD, Meena N, Rita S. Protective effect of Phyllanthus fraternus against cyclophosphamide induced nephrotoxicity in rats. Int J Basic Clin Pharmacol 2017;6:984-9.
29. Khan S, Yusufi AN. Effect of benzene on the enzymes of carbohydrate metabolism, brush border membrane (BBM) and oxidative stress in kidney and other rat tissues. Biol Med 2009;1:28-41.
30. Neghab M, Hosseinzadeh K, Hassanzadeh J. Early liver and kidney dysfunction associated with occupational exposure to sub-threshold limit value levels of benzene, toluene, and xylenes in unleaded petrol. Saf Health Work 2015;6:312-6.
31. Badham HJ, Renaud SJ, Wan J, Winn LM. Benzene-initiated oxidative stress: Effects on embryonic signaling pathways. Chem Biol Interact 2010;184:218-21.
32. Fenga C, Gangemi S, Teodoro M, Rapisarda V, Golokhvast K, Docea AO, et al 8-hydroxydeoxyguanosine as a biomarker of oxidative DNA damage in workers exposed to low-dose benzene. Toxicol Rep 2017;4:291-5.
33. Uzma N, Kumar BS, Hazari MA. Exposure to benzene induces oxidative stress, alters the immune response and expression of p53 in gasoline filling workers. Am J Ind Med 2010;53:1264-70.
34. Wilbur S, Wohlers D, Paikoff S, Keith LS, Faroon O. ATSDR evaluation of health effects of benzene and relevance to public health. Toxicol Ind Health 2008;24:263-398.
35. Kalf GF. Recent advances in the metabolism and toxicity of benzene. Crit Rev Toxicol 1987;18:141-59.
36. Bayliss D, Jinot J, Sonawane B. Toxicological Review of Benzene (CAS No. 71-43-2). In Support of Summary Information on the Integrated Risk Information System; EPA/635/R-02/001F. Washington, DC, USA: U.S. Environmental Protection Agency; 2002.
37. Derouiche S, Rezzag MO, Serouti A. Triazinone herbicide metribuzin induced acute liver injury: A study of animal model. J Acute Dis 2018;7:152-7.
38. Çavu?o?lu K, Yalçin E, Oruç E, Gür B, Çiçek F. The effects of grape seed extract against toxicity of benzene on liver and kidney tissues of albino mice: Biochemical evaluation. Turk J Biochem 2015;40:66-73.
39. Verma Y, Rana SV. Modulation of CYP4502E1 and oxidative stress by testosterone in liver and kidney of benzene treated rats. Indian J Exp Biol 2008;46:568-72.
40. Hetal R. Evaluation of benzene induced histopathological alteration in rat. Int J Adv Res 2016;4:563-7.
41. Sairazi SM, Sirajudeen KN, Muzaimi M, Swamy M, Asari MA, Sulaiman SA. Tualang honey attenuates kainic acid-induced oxidative stress in rat cerebellum and brainstem. Int J Pharm Pharm Sci 2017;9:155-62.
42. Abd-Elshakour A, El-Ebiarie AS, Ibrahim YH, Abd-Elmoneim AE, El-Mekawy AM. Effect of benzene on oxidative stress and the functions of liver and kidney in rats. J Environ Occup Sci 2015;4:34-9.
43. Elsayed AS. Hematotoxicity and oxidative stress caused by benzene. Pyrex J Biomed Res 2015;1:74-80.
44. Zarth AT, Murphy SE, Hecht SS. Benzene oxide is a substrate for glutathione S-transferases. Chem Biol Interact 2015;242:390-5.
45. Ahmad S, Agrawal R, Agrawal DK, Rao GS. Bioreactivity of glutathionyl hydroquinone with implications to benzene toxicity. Toxicology 2000;150:31-9.
46. Chikhi I, Allali H, Dib MA, Medjdoub H, Tabti B. Antidiabetic activity of aqueous leaf extract of Atriplex halimus L. (Chenopodiaceae) in streptozotocin-induced diabetic rats. Asian Pac J Trop Dis 2014;4:181 4.
47. Kaouachi A, Derouiche S. Phytochemical analysis, DPPH antioxidant activity and Acute toxicity of bark aqueous extracts of Pinus halepensis. Res J Chem Env Sci 2018;6:86-91.
48. Mohammedi Z. Resistance, pharmacology properties and nutritional value of a shrub from arid environments Atriplex halimus. Res J Med Plant 2016;10:10-8.
49. Mohammadi A, Ahmadizadeh M. Effects of antioxidants on xenobiotics-induced nephrotoxicity. J Renal Inj Prev 2018;7:56-7.
50. Cottone S, Lorito MC, Riccobene R, Nardi E, Mulè G, Buscemi S, et al. Oxidative stress, inflammation and cardiovascular disease in chronic renal failure. J Nephrol 2008;21:175-9.
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