• KRISHNAMOORTHI R Department of Botany, Bharathidasan University, Trichy, Tamil Nadu, India.
  • SIVAKUMAR SR Department of Botany, Bharathidasan University, Trichy, Tamil Nadu, India.


Objective: The objective of this study was to evaluate the antifungal activity of seaweed extracted protein against the pathogenic fungi.

Methods: Antifungal activity of seaweed Ulva lactuca L. extracted protein was determined against pathogenic fungi such as Alternaria solani, Aspergillus clavatus, Aspergillus niger, Aspergillus flavus, and Fusarium oxysporum by disk diffusion Method. Then, the potentially active protein was determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and circular dichroism (CD) spectroscopy.

Results: Seaweeds extracted protein checked for the antifungal activity against A. clavatus, A. solani, and A. flavus better activity compared to standard amphotericin-B and CD spectroscopy. Analysis of the extracts divulges the presence of entire protein compounds.

Conclusions: This study extracted seaweed protein sufficient of antifungal activity opposed to antifungal pathogens as compared with the standard. This is first report an activity of seaweed extracted protein against the plant and human pathogenic fungus bearing agricultural important.

Keywords: Bovine, Dichroism, Diffusion, Electrophoresis, Fungus, Protein.


1. Waldvogel FA. Infectious diseases in the 21st century: Old challenges and new opportunities. Int J Infect Dis 2004;8:5-12.
2. Cho SH, Kang SE, Cho JY, Kim AR, Park SM, Hong YK, et al. The antioxidant properties of brown seaweed (Sargassum siliquastrum) extracts. J Med Food 2007;10:479-85.
3. Kolsi RB, Frikha D, Jribi I, Hamza A, Feki L, Belghith K. Screening of antibacterial and anti fongical activity in marine macro algae and magnoliophytea from the coast of tunisha. Int J Pharm Pharm Sci 2015;7:47-51.
4. Fitzgerald RJ, Harnedy PA. In vitro assessment of the cardioprotective, antidiabetic and antioxidant potential of Palmaria palmata protein hydrolysates. J Appl Phycol 2013;25:1793-803.
5. Heffernan N, Thomas S, Villa AS, Richard F, Nigel B. Phenolic content and antioxidant activity of fractions of obtained from selected Irish macroalgae species (Laminaria digitata, Fucus serratus, Gracilaria gracilis and Codium fragile). J Appl Phycol 2014;27:519-30.
6. Magalhaes KD, Costa LS, Fidelis GP, Oliveira RM, Nobre LT, Dantas-Santos N, et al. Anticoagulant, antioxidant and antitumor activities of heterofucans from the seaweed Dictyopteris delicatula. Int J Mol Sci 2011;12:3352-65.
7. Mancini-Filho J, Novoa AV, González AE, de Andrade-Wartha ER, de O e Silva AM, Pinto JR, et al. Free phenolic acids from the seaweed Halimeda monile with antioxidant effect protecting against liver injury. Z Naturforsch C 2009;64:657-63.
8. Higuera AR, Corona LQ, Castro NP, Ceballos GC, Garcia AM, Flores ME. Antioxidant enzyme gene expression and antihypertensive effect of seaweeds Ulva linza and Lessonia trabeculata in rats feed a high- fat and high- sucrose diet. J Appl Phycol 2013;26:597-605.
9. Sellimi S, Kadri N, Barragan-Montero V, Laouer H, Hajji M, Nasri M, et al. Fucans from a tunisian brown seaweed Cystoseira barbata: Structural characteristics and antioxidant activity. Int J Biol Macromol 2014;66:281-8.
10. Zhang CY, Wu WH, Wang J, Lan MB. Antioxidant properties of polysaccharide from the brown seaweed Sargassum graminifolium (Turn.), and its effects on calcium oxalate crystallization. Mar Drugs 2012;10:119-30.
11. Arasaki TF, Mino N, Kuroda M. The protein value in human nutrition of edible marine algae in Japan. Hydrobilogia 1984;116:513-4.
12. Morgan KC, Wright JL, Simpson FJ. Review of chemical constituents of the red alga Palmaria palmate (dulse). Econ Bot 1980;34:27.
13. Rajasekar T, Shamya AM, Joseph J. Screening of phytochemical, antioxidant activity and anti-bacterial activity of marine seaweeds. Int J Pharm Pharm Sci 2019;11:61.
14. Abdel-Fattah AF, Sary HH. Selective isolation of glycoprotein materials from the green seaweed Ulva lactuca. Pak J Biochem 1987;20:61-5.
15. Khanna P, Nag TN, Chandrajaia S, Mohan S. Process for isolation of insulin from plant source. US Patent 1976;3:945-88.
16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.
17. Frolund B, Griebe T, Nielsen PH. Enzymatic-activity in the activated-sludge floc matrix. Appl Microbiol Biotechnol 1995;43:755-61.
18. Whitford D. Proteins: Structure and Function. Hoboken, New Jersey, USA: Wiley; 2005.
19. Krishnamoorthi R, Ayyadurai V, Marimuthu C. In-vitro antifungal activity of Naravelia zeylanica DC (Linn). J Nat Prod Plant Resour 2016;6:30-4.
20. Barot M, Kumar JI, Kumar RN. Bioactive compound and antifungal activity of three different seaweed species Ulva lactuca, Sagassum renerrimum and Laurencia obtusa collected from OKha coast, Western India. J Coast Life Med 2016;4:284-9.
21. Wang SB, Hu Q, Sommerfeld M, Chen F. An optimized protocol for isolation of soluble proteins from microalgae for two-dimensional gel electrophoresis analysis. J Appl Phycol 2003;15:485-96.
22. Kelly SM, Jess TJ, Price NC. How to study proteins by circular dichroism. Biochim Biophys Acta 2005;1751:119-39.
23. Perez-Iratxeta C, Andrade-Navarro MA. K2D2: Estimation of protein secondary structure from circular dichroism spectra. BMC Struct Biol 2008;8:25.
15 Views | 26 Downloads
How to Cite
R, K., and S. SR. “ANTIFUNGAL ACTIVITY OF SEAWEED ULVA LACTUCA L. EXTRACTED CRUDE PROTEIN AGAINST PATHOGENIC FUNGI”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 393-6,
Original Article(s)