IN VITRO ANTHELMINTIC ACTIVITY OF MAYTENUS EMARGINATA STEM BARK ON INDIAN ADULT EARTHWORM
Objective: The development of anthelmintic activity and the high cost of synthetic anthelmintic drugs it leads to the evaluation of medicinal plants as an alternative source of anthelmintics. In the current study, in vitro experiments were conducted to determine the possible anthelmintic effects of crude methanolic, aqueous, and hydroalcoholic extracts of the stem bark of Maytenus emarginata on eggs and adult Pheretima posthuma and Ascaridia galli.
Methods: Three concentrations (10, 25, and 50 mg/ml) of methanolic, aqueous, and hydroalcoholic extracts of plant M. emarginata were studied in activity which involved the determination of the time of paralysis (called as vermifuge) and time of death (called as vermicidal) of the worms. Piperazine citrate in same concentration as those of extract was included as standard reference and normal saline water with 1% carboxymethyl cellulose as control.
Results: The methanolic, aqueous, and hydroalcoholic extracts exhibited significant anthelmintic activity at a concentration of 50 mg/ml. Peak activity was exhibited by the methanolic extract at a concentration of 50 mg/ml.
Conclusion: The overall findings of the present study have shown that M. emarginata contain possible anthelmintic compounds and further evaluation of these plants should be carried out. The traditional use of anthelmintic activity of this plant is genuine.
2. Hossain E, Chandra G, Nandy AP, Mandal SC, Gupta JK. Anthelmintic effect of a methanol extract of leaves of Dregea volubilis on Paramphistomum explanatum. Parasitol Res 2012;110:809-84.
3. Tripathi KD. Essentials of Medical Pharmacology. 6th ed. New Delhi, India: Jaypee Brothers Medical Publishers (P) Ltd.; 2008. p. 808-10.
4. Patsnaik AK, Kodati D, Pareta SK, Patra KC, Harwansh RK. Analgesic and anti-inflammatory activities of Buchanania lanzan Spreg. Roots. Res J Pharm Biol Chem 2011;2:419-25.
5. CSIR. The Wealth of India, A Dictionary of Raw Materials and Industrial Products. Vol. 2. New Delhi, India: CSIR; 1997. p. 81.
6. Waller PJ, Thamsborg SM. Nematode control in green ruminant Production systems. Trends Prasitol 2004;20:493-7.
7. Sagwan S, Rao DV, Sharma RA. Maytenus emarginata (Willd.): A promising drug for cancer therapy. Asian J Pharm Clin Res 2011;4:177-80.
8. Ajaiyeoba EO, Onocha PA, Olarenwaju OT. In vitro anthelmintic properties of Buchholzia coriaceae and Gynandropsis gynandra extract. Pharm Bio 2001;39:217-20.
9. Kumar T, Alexander A, Dewangan D, Nagori K. Anthelmintic activity of the whole plant of Bauhinia purpurea (Linn). Asian J Pharm Clin Res 2011;4:110-1.
10. Kosalge SB, Fursule RA. Investigation of in vitro anthelmintic activity of Thespesia lampas (Cav.). Asian J Pharm Clin Res 2009;2:69-71.
11. Kundu S, Roy S, Nandi S, Ukil B, Larisha ML. In vitro anthelmintic effects of Senna Occidentalis (L.) Link (Leguminosae) on rat tapeworm Hymenolepis diminuta. Int J Pharm Pharm Sci 2015;7:268-71.
12. Chatterjee KD. Parasitology, Protozoology and Helminthology. 6th ed. Calcutta, India: Guha Ray Sree Saraswaty Press Ltd.; 1967. p. 168-9.
13. Vigar Z. Atlas of Medical Parasitology. 2nd ed. Singapore: P.G. Publishing House; 1984. p. 216.
14. Daniel WW. Biostatistics, A Foundation for the Analysis in the Health Sciences. 7th ed. Singapore: John Wiley and Sons (Asia) Pvt. Ltd.; 2004. p. 312.
15. Martin RJ. ?- Aminobutyric acid and piperazine activated single channel currents from Ascaris suum body muscle. Br J Pharm 1985;84:l445-61.
16. Niezen JH, Waghorn GC, Charlestone WA, Waghorn GC. Growth and gastrointestinal parasitism in lambs grazing either Lucerne (Medicago sativa) or Sulla (Hedysarum coronarium), which contains condensed tannins. J Agri Sci 1995;125:281-9.
17. Bate-Smith EC. The phenolic constituents of plants and their taxonomic significance, dicotyledons. Bot J Linn Soci 1968;60:325-56.
18. Martin RJ. Mode of actions of anthelmintic drugs. Vet J 1997;154:11-34.
19. Athnasiadou S, Kyriazakis F, Jackson RL. Coop. Direct anthelmic effect of condensed tannins towards different gastrointestinal nematodes of sheep: In vitro and in vivo studies. Vet Parasitol 2001;99:205-19.
20. Thomson DP, Geary TG. The structure and function of helminth surfaces. In: Marr JJ, editor. Biochemistry and Molecular Biology of Parasites. 1st ed. New York; Academic Press; 1995. p. 203-32.
21. Satyavati GV. Use of plants drugs in Indian traditional system of medicines and their relevance to primary health care. In: Farnsworth NR, Wagner H, editors. Economic and Medicinal Plant Research. London: Academic Press Ltd.; 1990. p. 190.
This work is licensed under a Creative Commons Attribution 4.0 International License.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.