ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF LUDWIGIA PARVIFLORA L. AGAINST STANDARD BACTERIAL STRAINS AND COMPARISON OF ITS ACTIVITY WITH THAT OF STANDARD ANTIBIOTICS

  • SHIBU GEORGE Department of Zoology, St. Aloysius College, (Affiliated to Mahatma Gandhi University, Kottayam, Kerala), Alappuzha, Kerala, India.
  • MEVLIN JOY St. John’s Research Institute, Bengaluru, Karnataka, India

Abstract

Objective: The objective of this study was to evaluate the antimicrobial activity of methanolic extract of Ludwigia parviflora L. using standard bacterial strains and compare its activity with that of standard antibiotics.


Methods: The antibacterial activity and antibiotic susceptibility tests were done by disk diffusion method using MTCC bacterial strains.


Results: The study revealed that the methanolic extract of the whole plant of L. parviflora L. was effective to inhibit the growth of Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli. Among the tested strains, S. aureus, P. aeruginosa, K. pneumoniae, and E. coli were more susceptible to the methanolic extract of L. parviflora than the commonly using antibiotic tetracycline 30 mcg. The activity of methanolic extract was also higher than the activity of gentamicin 10 mcg against the P. aeruginosa.


Conclusion: The study concluded that the crude methanolic extract of the whole plant of L. parviflora L. is a good source for antibacterial agent against S. aureus, P. aeruginosa, K. pneumoniae, and E. coli. Hence, this plant can be used as a natural alternative to the common antibiotics such as gentamicin and tetracycline against common bacterial infections after validating its pharmacological and toxicological activities.

Keywords: Antimicrobial activity, Ludwigia parviflora, Antibiotics, Methanolic extract, Disk diffusion method, Gentamicin, Tetracycline.

Author Biography

SHIBU GEORGE, Department of Zoology, St. Aloysius College, (Affiliated to Mahatma Gandhi University, Kottayam, Kerala), Alappuzha, Kerala, India.

Assistant Professor in Zoology

References

1. Neeraj M, Behal KK. Antimicrobial activity of some spices against selected microbes. Int J Pharm Pharm Sci 2010;2:18796.
2. Hayam MI, Ferial MA. Antibacterial activity of some medicinal plant extracts. Int J Biol Biomol Agric Food Biotechnol Eng 2014;8:1168-73.
3. Bijeshmon PP, Shibu G. Antimicrobial activity and powder microscopy of the flowers of Tabernaemontana divaricata R. Br. Indo Am J Pharm Res 2014;4:1601-5.
4. Rosina K, Barira I, Mohd A, Shazi S, Anis A, Manazir AS, et al. Antimicrobial activity of five herbal extracts against multi drug resistant (MDR) strains of bacteria and fungus of clinical origin. Molecules 2009;14:586-97.
5. Shibumon G, Benny PJ. Antibacterial potency of fruit extracts of Flacourtia inermis against multidrug resistant strains and comparison of its activity with that of standard antibiotics. Int J Pharm Sci Biotechnol 2010;1:96-9.
6. Subhas CM, Harsha R, Dinesha R, Thammanna GS. Antibacterial activity of Coleus aromaticus leaves. Int J Pharm Pharm Sci 2010;2:63 6.
7. Akinyemi KO, Oluwa OK, Omomigbehin EO. Antimicrobial activity of crude extracts of three medicinal plants used in south-west Nigerian folk medicine on some food borne bacterial pathogens. Afr J Trad Complement Alternat Med 2006;3:13-22.
8. Kong JM, Goh NK, Chia LS, Chia TF. Recent advances in traditional plant drugs and orchids. Acta Pharm 2003;24:7-21.
9. Datta SC, Banerjee AK. Useful weeds of West Bengal rice fields. Econ Bot 1979;32:297-310.
10. Mohammad SQ, Venkateshwar R, Kumar GS, Lubna N. Ethnobotanical study on medicinal plants used by traditional health practitioners and villagers of Garhphulghar Gram, Chhattisgarh, India. Asian J Pharm Res 2017;7:98-105.
11. Kirby MD, Bauer AW, Sherris JC, Turck M. Antibiotic susceptibility testing by standardized single disc diffusion method. Am J Clin Pathol 1966;45:493-6.
12. Selvamuthu B, Seetharaman S, Indra V, Daisy A. Antibacterial activity of methanolic extract of Ludwigia perennis leaves. World J Pharm Pharm Sci 2016;5:1186-93.
Statistics
19 Views | 40 Downloads
How to Cite
GEORGE, S., and M. JOY. “ANTIMICROBIAL ACTIVITY OF METHANOLIC EXTRACT OF LUDWIGIA PARVIFLORA L. AGAINST STANDARD BACTERIAL STRAINS AND COMPARISON OF ITS ACTIVITY WITH THAT OF STANDARD ANTIBIOTICS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 430-2, https://innovareacademics.in/journals/index.php/ajpcr/article/view/30818.
Section
Original Article(s)