GAS CHROMATOGRAPHY-MASS SPECTROMETRIC ANALYSIS AND IDENTIFICATION OF BIOACTIVE CONSTITUENTS OF CATHARANTHUS ROSEUS AND ITS ANTIOXIDANT ACTIVITY

  • JYOTI RANI Department of Botany, Punjabi University, Patiala, Punjab, India.
  • MANISH KAPOOR Department of Botany, Punjabi University, Patiala, Punjab, India.

Abstract

Objective: The main objectives of this study were analysis of the phytochemicals produced by two different Catharanthus roseus morphotypes, i.e., pink and white flowered and evaluate it morphologically and phytochemically in terms of total phenolic content (TPC), total flavonoid content (TFC), antioxidant properties, and gas chromatography-mass spectrometry (GC-MS) analysis.


Methods: Methanolic extracts of both morphotypes were prepared by Soxhlet apparatus. After extraction, the extracts were filtered and solvent removed by rotatory evaporator. TPC was determined by Folin–Ciocalteu reagent method and TFC was estimated by aluminum chloride colorimetric method. Antioxidant and free radical scavenging activities were estimated by superoxide dismutase and 1,1-diphenyl-2-picrylhydrazyl assay. GC-MS analysis was performed at Central Instrumentation Laboratory/ SAIF, Panjab University, Chandigarh.


 Results: Pink-flowered C. roseus showed highest activities in terms of TPC, TFC, and antioxidant activity as compared to white-flowered C. roseus. 42 different bioactive compounds were detected in the methanolic extract of pink, while only 7 compounds were identified in white-flowered C. roseus. The identification was performed by GS-MS analysis mainly based on retention time, peak area, molecular formula, and molecular weight.


Conclusion: The finding indicated that the pink-flowered C. roseus was phytochemically superior then the white one.

Keywords: Catharanthus roseus, Total phenolic content, Total flavonoid content, Antioxidant properties, Gas chromatography-mass spectrometry.

Author Biography

JYOTI RANI, Department of Botany, Punjabi University, Patiala, Punjab, India.

ASSOCIATE PROFESSOR

DEPARTMENT OF BOTANY, PUNJABI UNIVERSITY, PATIALA-147002 PUNJAB

References

1. Peter MP, Raj JY, Sicis VP, Joy V, Saravanan J, Sakthivel S. GC MS analysis of bioactive components on the leaves extract of Stylosanthes fruticosa-a potential folklore medicinal plant. Asian J Plant Sci Res 2012;2:243-53.
2. Azalework HG, Sahabjada, Jafri A, Arshad MD, Malik T. Phytochemical investigation, GC-MS profile and antimicrobial activity of a medicinal plant Ruta graveolens L. from Ethiopia. Int J Pharm Pharm Sci 2017;9:21-6.
3. Newman DJ, Cragg GM, Snadder KM. Natural products as sources of new drugs over the Newman period, 1981-2002. J Nat Prod 2003;66:1022-37.
4. Oluduro AO. Evaluation of antimicrobial properties and nutritional potentials of Moringa oleifera Lam. Leaf in South-Western Nigeria. Malays J Microbiol 2012;8:59-67.
5. Velmurugan P, Kamaraj M, Prema D. Phytochemical constituents of Cadaba trifoliata Roxb. root extract. Int J Phytomed 2010;2:379.
6. Christopher L, Abboah-Offei O. Anticancer properties of some ornamental plants on KNUST campus, Kumasi, Ghana. Int J Phytopharmacol 2011;5:366-70.
7. Jaleel CA, Gopi R, Sankar B, Manivannan P, Kumar KA, Sridharan R, et al. Studies on germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus seedlings under salt stress. S Afr J Bot 2007;73:190-5.
8. Rashmi R, Trivedi MP. Assessment of variations in different cultivars of Catharanthus roseus by using restriction endonuclease and rapid PCR. Int J Pure Appl Biosci 2014;2:336-242.
9. Patil PJ, Ghosh JS. Antimicrobial activity of Catharanthus roseus–a detailed study. Br J Pharmacol Toxicol 2010;1:40-4.
10. Sain M, Sharma V. Catharanthus roseus (an anti-cancerous drug yielding plant)-a review of potential therapeutic properties. Int J Pure Appl Biosci 2013;1:139-42.
11. Jacobs DI, Snoeijer W, Hallard D, Verpoorte R. The Catharanthus alkaloids: Pharmacognosy and biotechnology. Curr Med Chem 2004;11:607-28.
12. Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. J Enol Vitic 1965;16:144-58.
13. Park YS, Jung ST, Kang SG, Heo BG, Arancibia-Avila P, Toledo F, et al. Antioxidants and proteins in ethylene-treated kiwifruits. Food Chem 2008;107:640-8.
14. Kono Y. Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch Biochem Biophys 1978;186:189-95.
15. Blois MS. Antioxidant determination by the use of a stable free radical. Nature 2002;26:1199-200.
16. Gomez LA, Gomez AA. Statistical Procedure for Agricultural Research. 3rd ed. Singapore, MA: John Wiley and Sons; 1984.
17. Snedecor GW, Cochran WG. Statistical Method. 5th ed. Ames, Iowa: Iowa State University Press; 1980. p. 456.
18. Burton GN, Devane EM. Estimating heritability in fall Fescue (Festuca arundiancea L.) from replicated clonal materials. Agron J 1953;45:478 81.
19. Hussein HM. Analysis of trace heavy metals and volatile chemical compounds of Lepidium sativum using atomic absorption spectroscopy, gas chromatography-mass spectrometric and fourier-transform infrared spectroscopy Res J Pharm Biol Chem Sci 2016;2529-55.
20. Ajayi GO, Olagunju JA, Ademuyiwa O, Martins OC. GC-MS analysis and phytochemical screening of ethanolic root of Plumbago zeylanica (Linn.). J Med Plants Res 2011;5:1756-61.
21. Karthikeyan AV, Sudan I. GC-MS profile of in-vivo and in-vitro shoots of Cleome gynandra L. Int J Pharm Pharm Sci 2017;9:21-6.
22. Archana D, Dixitha M, Santhy M. Antioxidant and anti-clastogenic potential of Piper longum L. Int J Appl Pharm 2015;7:11-4.A
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RANI, J., and M. KAPOOR. “GAS CHROMATOGRAPHY-MASS SPECTROMETRIC ANALYSIS AND IDENTIFICATION OF BIOACTIVE CONSTITUENTS OF CATHARANTHUS ROSEUS AND ITS ANTIOXIDANT ACTIVITY”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 461-5, https://innovareacademics.in/journals/index.php/ajpcr/article/view/30865.
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