HYPERTHERMIA EFFECT ON HUMAN NORMAL BREAST (MCF-10A) AND CANCER (MDA-MB 231 AND MCF-7) CELLS

  • ASITA ELENGOE Department of Biotechnology, Faculty of Science, Lincoln University College, 47301 Petaling Jaya, Selangor, Malaysia,
  • NOOR JAHAN BANU MOHAMMED ALITHEEN Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia,
  • SALEHHUDDIN HAMDAN Department of Biosciences and Health Sciences, Faculty of Science, Universiti Teknologi Department of Biosciences and Health Sciences, Faculty of Science, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia.Malaysia, 81310 Skudai, Johor, Malaysia.

Abstract

Objective: In this study, the hyperthermia effect on the viability of human normal breast (MCF-10A) and cancer (MDA-MB 231 and MCF-7) cells was evaluated by MTT assay.


Methods: Cells were exposed to heat at 38ºC, 39ºC, 40ºC, 41ºC, 42ºC, 43ºC, and 44ºC for five different durations of heat exposure (0.5, 1, 2, 3, and 4 h). Breakpoint temperatures of MCF-10A, MDA-MB 231, and MCF-7 were determined using cumulative equivalent 43°C (CEM43) model. This model was first time used to calculate thermal isoeffect dose (TID) for MCF-10A, MDA-MB 231, and MCF-7.


Results: MCF-10A started to die at 42°C for 3 h while MDA-MB 231 and MCF-7 need a temperature of 38°C for 0.5 h; thus, they were identified as the threshold temperatures in CEM43 model. Furthermore, the effect of “43°C incubator 2 h” had similar total thermal dose as “44°C incubator 0.5 h” for MDA-MB 231 and MCF-7. In addition, “43°C incubator 3 h” effect had also almost the same thermal dose as “44°C incubator 1 h” for MDA-MB 231 and MCF-7.


Conclusion: A better understanding of the significant correlations between CEM43 and response parameters in clinical trials could be useful to treat breast cancer patients.

Keywords: Hyperthermia, MCF-10A, MDA-MB 231, MCF-7, cumulative equivalent 43°C model, Thermal isoeffect dose

References

1. American Cancer Society. Cancer Facts and Figures. Atlanta, Georgia: American Cancer Society; 2014.
2. Rukminingsih F, Andayani TM, Rahmawati F, Widayati K. Health-related quality of life in early breast cancer patients with hormone responsive. Int J Pharm Pharm Sci 2018;10:47-9.
3. Al Rashid MD, Bharawaj PV, Majumder S, Mandal V, Pal M, Mandal SC, et al. Antioxidant and anticancer activty of extract and fractions obtained from Diospyros melanoxylon roxb. leaves and correlation with their polyphenolic profiles. Int J Pharm Pharm Sci 2018;10:7-16.
4. Chalkidou A, Simeonidis K, Angelakeris M, Samaras T, Martinez-Boubeta C, Balcells, L. In vitro application of Fe/MgO nanoparticles as magnetically mediated hyperthermia agents for cancer treatment. J Magn Magn Mater 2011;323:775-80.
5. van der Zee J. Heating the patient: A promising approach? Ann Oncol 2002;13:1173-84.
6. Hildebrandt B, Wust P, Ahlers O, Dieing A, Sreenivasa G, Kerner T, et al. The cellular and molecular basis of hyperthermia. Crit Rev Oncol Hematol 2002;43:33-56.
7. Issels RD. Hyperthermia adds to chemotherapy. Eur J Cancer 2008;44:2546-54.
8. Urano M, Kuroda M, Nishimura Y. For the clinical application of thermochemotherapy given at mild temperatures. Int J Hyperthermia 1999;15:79-107.
9. Morris CC, Myers R, Field SB. The response of the rat tail to hyperthermia. Br J Radiol 1977;50:576-80.
10. Sapareto SA, Dewey WC. Thermal dose determination in cancer therapy. Int J Radiat Oncol Biol Phys 1984;10:787-800.
11. Omar RA, Lanks KW. Heat shock protein synthesis and cell survival in clones of normal and simian virus 40-transformed mouse embryo cells. Cancer Res 1984;44:3976-82.
12. Alley MC, Scudiero DA, Monks A, Hursey ML, Czerwinski MJ, Fine DL, et al. Feasibility of drug screening with panels of human tumor cell lines using a microculture tetrazolium assay. Cancer Res 1988;48:589-601.
13. Muruganandam L, Maheswari R. Anti-cancer studies of selective mannich bases by in silico method. Int J Curr Pharm Res 2018;10:81-5.
14. Yonezawa M, Otsuka T, Matsui N, Tsuji H, Kato KH, Moriyama A, et al. Hyperthermia induces apoptosis in malignant fibrous histiocytoma cells in vitro. Int J Cancer 1996;66:347-51.
15. Fukao H, Ikeda M, Ichikawa T, Inufusa H, Okada K, Ueshima S, et al. Effect of hyperthermia on the viability and the fibrinolytic potential of human cancer cell lines. Clin Chim Acta 2000;296:17-33.
16. Ellis RJ, van der Vies SM. Molecular chaperones. Annu Rev Biochem 1991;60:321-47.
17. Williams RS, Thomas JA, Fina M, German Z, Benjamin IJ. Human heat shock protein 70 (hsp70) protects murine cells from injury during metabolic stress. J Clin Invest 1993;92:503-8.
18. Gabai VL, Kabakov AE. Tumor cell resistance to energy deprivation and hyperthermia can be determined by the actin skeleton stability. Cancer Lett 1993;70:25-31.
19. Watanabe M, Suzuki K. Heat sensitivity of human cancer cells and abnormal expression of heat shock protein 70. Gan No Rinsho 1989;35:1512-6.
20. Takasu T, Lyons JC, Park HJ, Song CW. Apoptosis and perturbation of cell cycle progression in an acidic environment after hyperthermia. Cancer Res 1998;58:2504-8.
21. Urano M, Rice L, Epstein R, Suit HD, Chu AM. Effect of whole-body hyperthermia on cell survival, metastasis frequency, and host immunity in moderately and weakly immunogenic murine tumors. Cancer Res 1983;43:1039-43.
22. Song CW, Lokshina A, Rhee JG. Implication of blood flow in hyperthermic treatment of tumors. IEEE Trans BME 1984;31:9-16.
23. Marder J, Eylath U, Moskovitz E, Sharir R. The effect of heat exposure on blood chemistry of the hyperthermic rabbit. Comp Biochem Physiol A Comp Physiol 1990;97:245-7.
24. Han JS, Storck CW, Wachsberger PR. Acute extracellular acidification increases nuclear associated protein levels in human melanoma cells during 42°C hyperthermia and enhances cell killing. Int J Hyperthermia 2002; 18: 404-15.
25. Dewhirst MW, Viglianti BL, Lora-Michiels M, Hanson M, Hoopes PJ. Basic thermal dosimetry and thermal thresholds for tissue damage from hyperthermia. Int J Hyperthermia 2003;19:267-94.
Statistics
24 Views | 94 Downloads
How to Cite
ELENGOE, A., N. J. B. MOHAMMED ALITHEEN, and S. HAMDAN. “HYPERTHERMIA EFFECT ON HUMAN NORMAL BREAST (MCF-10A) AND CANCER (MDA-MB 231 AND MCF-7) CELLS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 512-5, https://innovareacademics.in/journals/index.php/ajpcr/article/view/30956.
Section
Original Article(s)