CARDIOPROTECTIVE EFFECT OF FISETIN ON LYSOSOMAL ENZYME ACTIVITIES OF NORMAL AND ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION IN MALE WISTAR RATS

  • MYTHILY PANNEERSELVAM Department of Biochemistry, Mohamed Sathak College of Arts and Science, Chennai, Tamil Nadu, India.
  • DEVIKA P. T Department of Biochemistry, Mohamed Sathak College of Arts and Science, Chennai, Tamil Nadu, India.

Abstract

Objective: This study aims to evaluate the cardioprotective effect of fisetin on change in the activities of lysosomal enzymes in isoproterenol (ISO)- induced myocardial infarction (MI) in male albino Wistar rats.


Methods: Rats were orally pretreated with fisetin (30 mg/kg body weight) daily for 30 days. After the pretreatment period, ISO (100 mg/kg body weight) was subcutaneously administered to rats at intervals of 24 h for 2 consecutive days.


Results: The activities of β-glucuronidase, β-N-acetylglucosaminidase, β-galactosidase, cathepsin-B, and cathepsin-D were significantly (*p<0.05) increased in serum and the heart of ISO-induced rats. Pre-treatment with fisetin daily for 30 days to ISO-induced rats significantly prevented these activities and restored their activities to near normal. Oral treatment with fisetin (30 mg/kg body weight) to normal control rats did not show any significant effect.


Conclusion: We have concluded that fisetin protects the β-glucuronidase, β-N-acetylglucosaminidase, β-galactosidase, cathepsin-B, and cathepsin-D against ISO-induced MI. The observed effects might be due to the free radical-scavenging and membrane-stabilizing effect of fisetin.

Keywords: Myocardial infarction, Isoproterenol, Fisetin and Lysosomal enzymes.

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PANNEERSELVAM, M., and D. P. T. “CARDIOPROTECTIVE EFFECT OF FISETIN ON LYSOSOMAL ENZYME ACTIVITIES OF NORMAL AND ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION IN MALE WISTAR RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 516-9, https://innovareacademics.in/journals/index.php/ajpcr/article/view/30974.
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