EVALUATION OF THE HISTOPATHOLOGICAL AND BIOCHEMICAL EFFECT OF ALOE VERA AQUEOUS EXTRACT ON DIABETES MELLITUS INDUCED BY STREPTOZOTOCIN IN RATS
Objective: Numerous plants have exciting pharmaceutical activities, but Aloe vera (AV) is likely to have many medicinal applications worldwide. Therefore, the present study focused on identifying the potential of AV aqueous extract to protect against complications of diabetes.
Methods: Fifty rats were divided into five groups. The first group, the normal group, received a vehicle solution. The second group, the AV group, received AV aqueous extract (1/2 ml/100 g body weight). The third group was the streptozotocin (STZ)-induced diabetic rat group (i.p. as a single dose), the fourth group was the ameliorative group (a week after STZ injection, rats were given the same daily oral dose of AV extract), and the fifth group was the protective group (rats were treated with the same dose of AV extract for 21 days, and on the 14th day after the start of AV treatment, STZ injection was performed). At the end of the experiment, changes in serum indices, such as insulin, glucose, aspartate transaminase (AST), alanine transaminase (ALT), urea and creatinine, and tissue antioxidants as well as histopathological alterations in the pancreas, liver, and kidney were evaluated.
Results: We found that STZ-treated animals displayed significant increases in tissue lipid peroxidation (LPO, as an indicator of oxidative stress), serum glucose, AST, ALT, urea, and creatinine, with a parallel decrease in the levels of serum insulin and tissue antioxidants. In accordance with these data, several histopathological alterations in the selected organs were observed. When diabetic animals received 1/2 ml/100 g body weight of AV extract, these deleterious effects were ameliorated.
Conclusion: In the current study, AV aqueous extract exhibited an ameliorative and protective effect against the oxidative damage and the associated complications that occurred in different rat organs due to diabetes mellitus induced by STZ.
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