RAPHANUS SATIVUS LINN. A NEW ANTINOCICEPTIVE FOR DIABETIC NEUROPATHY IN RATS DETERMINED BY RANDALL SELITTO APPROACH
Keywords:Raphanus sativus linn, Streptozotozin, type II DM, sciatic nerve, antinociceptive effect, Randall selitto method
Objective: The objective of the study was to evaluate the antinociceptive effect of Raphanus sativus Linn. using Randall Selitto method.
Methods: Streptozotocin, lard, casein, cholesterol, DL-methionine, yeast powder, quercetin, thiobarbituric acid, 2-nitrobenzoic acid (5, 5, Dithiobis), hematoxylin, and hydrogen peroxide were used. A diet rich in fat content was fed to the animals for a period of 2 weeks. After a stabilization period of 2weeks, the treatment period started and continued for a period of 8weeks. The nociceptive parameters were assessed once a week by Randall Selitto method and hot plate test. After treatment, the animals were sacrificed, and antioxidant parameters were assessed using sciatic nerve homogenate and histopathological analysis of sciatic nerve.
Results: Treatment R. sativus extract (RSE 100 mg/kg and 200 mg/kg) appreciably declined the levels of blood glucose in a dose-dependent manner, and it was comparable with standard quercetin. A significant increase in pain threshold levels was observed by the treatment RSE in hot plate method after the 4th week compared to diabetic control, and it was consistent until the end of treatment (p<0.01, p<0.001). In Randall Selitto method RSE produced a significant increase in paw withdrawal threshold after the 4th week compared to diabetic control, and it was consistently increased until the end of treatment. RSE (100 and 200 mg/kg) significantly restored the levels of antioxidant enzymes and decreased lipid peroxidation in a dose-dependent fashion in comparison with the diabetic control group. RSE (100 mg/kg and 200 mg/kg) attenuated the nerve degeneration and axonal swelling along with quercetin.
Conclusion: The findings from the current study showed the antinociceptive and antioxidant effect of R. sativus in neuropathic pain in diabetes.
Gutiérrez RM, Perez RL. Raphanus sativus (Radish): Their chemistry and biology. ScientificWorldJournal 2004;4:811-37.
Upadhyay PB, Roy S, Kumar A. Traditional uses of medicinal plants among the rural communities of Churu district in the Thar Desert, India. J Ethnopharm 2007;113:387-99.
Gilani AH, Ghayur MN. Pharmacological basis for the gut stimulatory activity of Raphanus sativus leaves. J Ethnopharm 2004;95:169-72.
Anjaneyulu M, Chopra K. Quercetin, a bioflavonoid, attenuates thermal hyperalgesia in a mouse model of diabetic neuropathic pain. Prog Neuropsychopharmacol Biol Psychiatry 2003;27:1001-5.
Beevi SS, Lakshmi NM, Gowda BB. Polyphenolics profile, antioxidant and radical scavenging activity of leaves and stem of Raphanus sativus Linn. Plant Foods Hum Nutr 2010;65:8-17.
Asna U, Vanitha RP, Shruthi D, Faiyaz A. Antioxidant properties and stability of Raphanus sativus extracts. J Pharm Res 2010;3:658-61.
Hidayati S, Sulistyawati R, Nurani LH. Regulation of ethyl acetate fraction from Moringa oleifera leaves to improve lipid metabolism and insulin sensitivity in type 2 diabetes. Int J Pharm Pharm Sci 2018;10:78 82.
Edwards JL, Vincent AM, Cheng HT, Feldman EL. Diabetic neuropathy: Mechanisms to management. Pharmacol Ther 2008;120:1-34.
Greene DA, Stevens MJ, Feldman EL. Diabetic neuropathy: Scope of the syndrome. Am J Med 1999;107:2S-8S.
Vinik AI, Mitchell BD, Maser RE, Freeman R. Diabetic autonomic neuropathy. Diabetes Care 2003;26:1553-79.
Barrière DA, Noll C, Roussy G, Lizotte F, Kessai A, Kirby K, et al. Combination of high-fat/high-fructose diet and low-dose streptozotocin to model long-term type-2 diabetes complications. Sci Rep 2018;8:424.
Bachhav R, Saudagar R. Effect of ethanolic flower extract of Spathodea campanulata on streptozotocin-induced diabetic neuropathy. Int J Pharm Pharm Sci 2018;10:64-9.
Konrad RJ, Mikolaenko I, Tolar JF, Liu K, Kudlow JE. The potential mechanism of the diabetogenic action of streptozotocin inhibition of pancreatic β-cell O-GlcNAc selective N-acetyl-b-D-glucosaminidase. Biochem J 2001;356:31-41.
Lenzen S. The mechanisms of alloxan- and streptozotocin-induced diabetes. Diabetologia 2008;51:216-26.
Orient Longmans. Raphanus sativus Linn. Indian Medicinal Plants. 4th ed. Hyderabad: Orient Longman private limited; 2006. p. 407.
Srinivasan K, Viswanad B, Asrat L, Kaul CL, Ramarao P. Combination of high-fat diet-fed and low-dose streptozotocin-treated rat: A model for type 2 diabetes and pharmacological screening. Pharmacol Res 2005;52:313-20.
Ramdas BP, Sangameswaran B, Popat BM, Shantaram GK. Attenuating effect of seeds of Adenanthera pavonina aqueous extract in neuropathic pain in streptozotocin-induced diabetic rats: An evidence of neuroprotective effects. Brazil J Pharm 2012;22:428-35.
Reed MJ, Meszaros K, Entes LJ, Claypool MD, Pinkett JG, Gadbois TM, et al. A new rat model of type 2 diabetes: The fat-fed, streptozotocin-treated rat. Metabolism 2000;49:1390-4.
Prem KN, Annamalai AR, Thakur RS. Antinociceptive property of Emblica officinalis Gaertn (Amla) in high fat fed/low dose streptozotocin-induced diabetic neuropathy. Indian J Exp Biol 2009;47:737-42.
Tsagareli MG, Tsiklauri N, Gurtskaia G, Nozadze I, Abzianidze E. The central nucleus of the amygdala is involved in tolerance to the antinociceptive effect of NSAIDs. Health 2010;2:62-6.
Miki S, Yoshinaga N, Iwamoto T, Yasuda T, Sato S. Antinociceptive effect of the novel compound OT-7100 in a diabetic neuropathy model. Eur J Pharmacol 2001;430:229-34.
Kanwaljit C, Anurag K, Richa S. Lycopene attenuates the diabetes-associated cognitive decline in rats. Life Sci 2008;83:128-34.
Mukherjee D, Roy SG, Bandyopadhyay A, Chattopadhyay A, Basu A, Mitra E, et al. Melatonin protects against isoproterenol-induced myocardial injury in the rat: Antioxidative mechanisms. J Pineal Res 2010;48:251-62.
Stevens MJ, Obrosova I, Xianghui C, Carol VH, Greene DA. Effects of DL-a-lipoic acid on peripheral nerve conduction, blood flow, energy metabolism, and oxidative stress in experimental diabetic neuropathy. Diabetes 2000;49:1006-15.
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