• ANITHA K Research scholar, JNTUA, Ananthapuramu, Andhra Pradesh, India.
  • SABAPATHI MOHANA LAKSHMI Department of Pharmacognosy, Sree Vidyanikethan College of Pharmacy, Tirupati, Andhra Pradesh, India.
  • SATYANARAYANA SV Research scholar, JNTUA, Ananthapuramu, Andhra Pradesh, India.


Objective: The present study was to explore the phytochemical analysis and antidiabetic potential of the root of Commiphora caudata in high-fat diet (HFD) streptozotocin-induced diabetic rats.

Methods: The ethanolic root extract of C. caudata at a dose of 400 mg/kg and 200 mg/kg was administered to diabetic rats. Glibenclamide (5 mg/kg) was used as standard drug.

Results: The data were statistically assessed using one-way ANOVA followed by Dennett’s multiple comparison tests. To unfold the mechanism, we studied all the biochemical parameters glucose, total cholesterol, triglycerides (TG), high-density lipoproteins (HDL), low-density lipoproteins (LDL), and very LDL (VLDL) and histopathological examination of the pancreatic tissue section. The ethanolic extracts of root of C. caudata showed significant reduce of the level of cholesterol, TG, LDL, VLDL, and significant increase in the serum level of HDL at 400 mg/kg rather than 200 mg/kg.

Conclusion: Further studies should look into the characterization and isolation of the constituents to know the exact mechanism of hypoglycemic activity. Statistical analyses of this screening method confirm that the proposed method is appropriate and it can be expected to improve basic idea to the researcher who is working in area-like antidiabetic activity.

Keywords: Commiphora caudata, Glibenclamide, High-fat diet streptozotocin, Cholesterol, Triglycerides, Low-density lipoproteins, and Very lowdensity lipoproteins.


1. Moneva MH, Dagogo-Jack S. Multiple drug targets in the management of type 2 diabetes. Curr Drug Targets 2002;3:203-21.
2. Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.
3. Yamada K, Nakata M, Horimoto N, Saito M, Matsuoka H, Inagaki N, et al. Measurement of glucose uptake and intracellular calcium concentration in single, living pancreatic beta-cells. J Biol Chem 2000;275:22278-83.
4. Dhar ML, Dhar MM, Dhawan BN, Mehrotra BN, Ray C. Screening of Indian plants for biological activity: I. Indian J Exp Biol 1968;6:232-47.
5. Gunatilaka AA, Balasubramaniam S. Biological importance of some herbal medicines. J Nat Sci Council 1978;8:87-207.
6. Gamble JS. Flora of Presidency of Madras. London: Adlod and Sons Ltd; 1992. p. 170-1.
7. Harborne AJ. Phytochemical methods: A guide to modern techniques of plant analysis. J Nat Sci Council 2008;3:4-7.
8. Kokate CK, Purohit AP, Gokhale SB. Pharmacognosy. Pune: Nirali Prakashan; 2008. p. 56-8.
9. OECD Guidelines for the Testing of Chemicals. Available from: http:// [Last accessed on 2016 Mar 20].
10. Zhang W, Zhao J, Wang J, Pang X, Zhuang X, Zhu X, et al. Hypoglycemic effect of aqueous extract of seabuckthorn (Hippophae rhamnoides L.) seed residues in streptozotocin-induced diabetic rats. Phytother Res 2010;24:228-32.
11. Singh RK, Mehta S, Jaiswal D, Rai PK, Watal G. Antidiabetic effect of Ficus bengalensis aerial roots in experimental animals. J Ethnopharmacol 2009;123:110-4.
12. Singh SK, Kesari AN, Gupta RK, Jaiswal D, Watal G. Assessment of antidiabetic potential of Cynodon dactylon extract in streptozotocin diabetic rats. J Ethnopharmacol 2007;114:174-9.
13. Si MM, Lou JS, Zhou CX, Shen JN, Wu HH, Yang B, et al. Insulin releasing and alpha-glucosidase inhibitory activity of ethyl acetate fraction of Acorus calamus in vitro and in vivo. J Ethnopharmacol 2010;128:154-9.
14. Ahmad W, Khan I, Khan MA, Ahmad M, Subhan F, Karim N, et al. Evaluation of antidiabetic and antihyperlipidemic activity of Artemisia indica Linn (aeriel parts) in streptozotocin induced diabetic rats. J Ethnopharmacol 2014;151:618-23.
15. Xu Z, Ju J, Wang K, Gu C, Feng Y. Evaluation of hypoglycemic activity of total lignans from Fructus arctii in the spontaneously diabetic goto-kakizaki rats. J Ethnopharmacol 2014;151:548-55.
16. Subhasree N, Kamella A, Kaliappan I, Agrawal A, Dubey GP. Antidiabetic and antihyperlipidemic activities of a novel polyherbal formulation in high fat diet/streptozotocin induced diabetic rat model. Indian J Pharmacol 2015;47:509-13.
17. Li Y, Huang TH, Yamahara J. Salacia root, a unique Ayurvedic medicine, meets multiple targets in diabetes and obesity. Life Sci 2008;82:1045-9.
18. Mohammadi J, Naik PR. Evaluation of hypoglycemic effect of Morus alba in an animal model. Indian J Pharmacol 2008;40:15-8.
19. Khanna AK, Chander R, Kapoor NK, Dhawan N. Hypolipidemic activity of picroliv in albino rats. Pytother Res 1994;8:403-7.
20. Lopes-Virella MF, Stone P, Ellis S, Colwell JA. Cholesterol determination in high-density lipoproteins separated by three different methods. Clin Chem 1977;23:882-4.
21. Carr TP, Andresen CJ, Rudel LL. Enzymatic determination of triglyceride, free cholesterol, and total cholesterol in tissue lipid extracts. Clin Biochem 1993;26:39-42.
22. Weggemans RM, Trautwein EA. Relation between soy-associated isoflavones and LDL and HDL cholesterol concentrations in humans: A meta-analysis. Eur J Clin Nutr 2003;57:940-6.
23. Kannadhasan R, Venkataraman S. Antidiabetic and antihyperlipidaemic activity of sedimental extract of Tinospora cordifolia in streptozotocin induced type 2 diabetes. Int J Pharm Pharm Sci 2012:520-7.
24. Aleykutty JD, Jyoti H. Effect of combination of two plant extracts on diabetes mellitus. Int J Pharm Pharm Sci 2018:49-52.
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How to Cite
K, A., S. M. LAKSHMI, and S. SV. “PROTECTIVE EFFECT OF ETHANOLIC ROOT EXTRACT OF COMMIPHORA CAUDATA AGAINST DIABETIC-INDUCED RATS IN HIGH-FAT DIET-STREPTOZOTOCIN MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 3, Feb. 2019, pp. 563-6, doi:10.22159/ajpcr.2019.v12i3.31083.
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