• GOZIF MOHAMMED N OMAR Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad – 431 004, India.
  • FADEL YOUSIF AL-ARABI Dr. Rafiq Zakaria College for Woman, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad – 431 001, India.
  • MANSOUR ABDULNABI H MEHDI Dr. Rafiq Zakaria College for Woman, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad – 431 001, India.
  • MADHUKAR M FAWADE Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad – 431 004, India.


Objectives: This study is to investigate the effect of oral Vitamins combined (A, C, and E) with metformin on the levels of fasting blood sugar (FBS), postprandial blood sugar (PPBS), hemoglobin (HbA1c), total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL), and low-density lipoprotein (LDL) in Type 2 diabetic patients.

Methods: A total of 32 patients with Type 2 diabetes were divided randomly into A and B groups, i.e., 16 per each group. Group A received metformin only twice a day while Group B received one tablet of Antox and metformin twice a day for 3 months. FBS, PPBS, HbA1c, TC, TG, LDL, and HDL were measured before and after the dose, and the results were analyzed statistically.

Results: A significant decreases in FBS, PPBS, HbA1c, TC, TG, and LDL whereas results showed increasing significance in HDL level that seen in the patients of Group B which received Antox and metformin compared to the group which received metformin only. In conclusion, the results indicate that daily consumption of supplementary vitamins with metformin leads to improving blood glucose and lipids in patients with Type 2 diabetes and reducing the risk of complications. Hence, the dose can be used combined Vitamins A, C, and E with metformin in the treatment of Type 2 diabetes by maintaining good glycemic control.

Keywords: Type 2 diabetes, Blood sugar, Serum lipids, Vitamins A, C and E.

Author Biography

GOZIF MOHAMMED N OMAR, Department of Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad – 431 004, India.

Department of  Biochemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad (India).


1. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2014;37 Suppl 1:S81-90.
2. Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.
3. Kamala S, Mohammed A, Swathi K, Venkateswarlu K, Ram MR. Evaluation of pharmacoeconomic direct cost in diabetes patients. Asian J Pharm Clin Res 2017;10:38-40.
4. Ting HH, Timimi FK, Boles KS, Creager SJ, Ganz P, Creager MA, et al. Vitamin C improves endothelium-dependent vasodilation in patients with non-insulin-dependent diabetes mellitus. J Clin Invest 1996;97:22-8.
5. Fadupin GT, Akpoghor AU, Okunade KA. A comparative study of serum ascorbic acid level in people with and without Type 2 diabetes in Ibadan, Nigeria. Afr J Med Med Sci 2007;36:335-9.
6. Afkhami-Ardekani M, Shojaoddiny-Ardekani A. Effect of Vitamin C on blood glucose, serum lipids and serum insulin in Type 2 diabetes patients. Indian J Med Res 2007;126:471-4.
7. Prajapat R, Bhattacharya I, Jakhalia A. Combined effect of Vitamin C and E dose on Type 2 diabetes patients. Adv Diabetes Metab 2017;5:21 5.
8. Matough FA, Budin SB, Hamid ZA, Alwahaibi N, Mohamed J. The role of oxidative stress and antioxidants in diabetic complications. Sultan Qaboos Univ Med J 2012;12:5-18.
9. Bonnefont-Rousselot D, Bastard JP, Jaudon MC, Delattre J. Consequences of the diabetic status on the oxidant/antioxidant balance. Diabetes Metab 2000;26:163-76.
10. Omar G, Mehdi M, AL-Arabi F. Management of diabetes and its complications through the role of antioxidants: Review. Res Rev Int J Multidiscip 2018;9:519-26.
11. Vinotha AT, Senthilkumaran P. Effect of enalapril and metformin on oxidative stress in newly diagnosed Type 2 diabetes mellitus and hypertensive patients. Asian J Pharm Clin Res 2015;9:69-72.
12. Srivatsan R, Das S, Gadde R, Manoj-Kumar K, Taduri S, Rao N, et al. Antioxidants and lipid peroxidation status in diabetic patients with and without complications. Arch Iran Med 2009;12:121-7.
13. Afkhami-Ardekani M, Vahidi AR, Borjian L, Borjian L. Effect of Vitamin C supplement on glycosylated hemoglobin in patients with Type 2 diabetes. J Shah Sad Univ 2003;10:15-8.
14. Ganesh ND, Chaudhari HV, Shrivastava M. Supplementation of Vitamin C reduces blood glucose and improves glycosylated hemoglobin in Type 2 diabetes mellitus: A randomized, double-blind study. Adv Pharmacol Sci 2011;2011:1-5.
15. Forghani B, Goharian V, Kassayian N, Amini M. The effect of Vitamin C supplements on serum lipoprotein levels in NIDDM patients. J M C Isfahan Univ 2001;2:95-100.
16. Eriksson J, Kohvakka A. Magnesium and ascorbic acid supplementation in diabetes mellitus. Ann Nutr Metab 1995;39:217-23.
17. Paolisso G, D’Amore A, Giugliano D, Ceriello A, Varricchio M, D’Onofrio F, et al. Pharmacologic doses of Vitamin E improve insulin action in healthy subjects and non-insulin-dependent diabetic patients. Am J Clin Nutr 1993;57:650-6.
18. Reaven PD, Herold DA, Barnett J, Edelman S. Effects of Vitamin E on susceptibility of low-density lipoprotein and low-density lipoprotein subfractions to oxidation and on protein glycation in NIDDM. Diabetes Care 1995;18:807-16.
19. Paolisso G, D’Amore A, Galzerano D, Balbi V, Giugliano D, Varricchio M, et al. Daily Vitamin E supplements improve metabolic control but not insulin secretion in elderly Type II diabetic patients. Diabetes Care 1993;16:1433-7.
20. Pazdro R, Burgess JR. The role of Vitamin E and oxidative stress in diabetes complications. Mech Ageing Dev 2010;131:276-86.
21. Iqbal S, Naseem I. Role of Vitamin A in Type 2 diabetes mellitus biology: Effects of intervention therapy in a deficient state. Nutrition 2015;31:901-7.
22. Hodis HN, Mack WJ, Dustin L, Mahrer PR, Azen SP, Detrano R, et al. High-dose B vitamin supplementation and progression of subclinical atherosclerosis: A randomized controlled trial. Stroke 2009;40:730-6.
23. Low-Income Americans and Racial and Ethnic Minorities Experience Disproportionately Higher Rates of Disease. U.S Medicine Report; 2009.
24. Cabrera-Valladares G, German MS, Matschinsky FM, Wang J, Fernandez-Mejia C. Effect of retinoic acid on glucokinase activity and gene expression and on insulin secretion in primary cultures of pancreatic islets. Endocrinology 1999;140:3091-6.
25. Ziouzenkova O, Orasanu G, Sharlach M, Akiyama TE, Berger JP, Viereck J, et al. Retinaldehyde represses adipogenesis and diet-induced obesity. Nat Med 2007;13:695-702.
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How to Cite
GOZIF MOHAMMED N OMAR, FADEL YOUSIF AL-ARABI, MANSOUR ABDULNABI H MEHDI, and MADHUKAR M FAWADE. “ANTIOXIDANT EFFECT OF VITAMINS COMBINED A, C, AND E DOSE ON TYPE 2 DIABETIC PATIENTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 4, Feb. 2019, pp. 51-54, doi:10.22159/ajpcr.2019.v12i4.31216.
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