• PRABHAKARAN K Department of Chemistry, Government Arts College, Ayyar Malai, Kulithalai – 639 120, Tamil Nadu, India.
  • JOHN BRITTO S Department of Rapinat Herbarium and Molecular Systematics, St. Joseph’s College, Tiruchirappalli, Tamil Nadu, India.
  • PREETHI J Department of Cellular and Molecular Biology, Lab-D05, Institute of Neurobiology, UNAM, Campus Juriquilla, Queretaro, Mexico.


Objective: Salvadora persica L. is a dense foliaceous evergreen shrub or small tree with diversified medicinal properties. The objective of this work was to do a comparative study on phytochemical composition between different plant parts of S. persica collected from the southern region of India.

Methods: The phytochemical analysis of ethyl acetate fraction of ethanolic extracts from leaves, tender stems and tree bark of S. persica was done by gas chromatography-mass spectrometry/mass spectrometry (GC-MS/MS). Also, the anti-bacterial and anti-fungal activity of extracts was analyzed in vitro by Disc-diffusion method.

Results: GC-MS/MS analysis of S. persica showed 29 phytocompounds. Among them, except for eugenol, caryophyllene, benzyl isothiocyanate, oleic acid, and fatty acid, the remaining 24 phytocompounds were newly reported in the present study. For the first time, a maximum amount of benzyl isothiocyanate (73.5%) was identified from tree bark extract of S. persica and this extract showed higher in vitro antimicrobial activity against gram-positive, gram-negative bacteria and fungi than leaves and tender stems.

Conclusion: The study demonstrated that benzyl isothiocyanate could be the major antimicrobial component in S. persica.

Keywords: Salvadora persica, Toothbrush tree, Gas chromatography-mass spectrometry/mass spectrometry, Benzyl isothiocyanate, Antimicrobial activity.


1. Verdine GL. The combinatorial chemistry of nature. Nature 1996;384:11-3.
2. Wessjohann LA. Synthesis of natural-product-based compound libraries. Curr Opin Chem Biol 2000;4:303-9.
3. Newman DJ, Cragg GM, Snader KM. Natural products as sources of new drugs over the period 1981-2002. J Nat Prod 2003;66:1022-37.
4. Chathurdevi G, Umagowrie S. Potent bioactive metabolites of Casuarina junghuhniana MIQ. Roots – A therapeutic approach. Int J Pharm Pharm Sci 2017;9:158-65.
5. Katewa SS, Chaudhary BL, Jain A. Folk herbal medicines from tribal area of Rajasthan, India. J Ethnopharmacol 2004;92:41-6.
6. Uddin I, Kanatas A. Oral health: Salvadora persica. Br Dent J 2014;216:98.
7. Ezmirly ST, Cheng JC, Wilson SR. Saudi Arabian medicinal plants: Salvadora persica. Planta Med 1979;35:191-2.
8. Al-Fatimi M, Wurster M, Schröder G, Lindequist U. Antioxidant, antimicrobial and cytotoxic activities of selected medicinal plants from yemen. J Ethnopharmacol 2007;111:657-66.
9. Khatak M, Khatak S, Siddqui AA, Vasudeva N, Aggarwal A, Aggarwal P. Salvadora persica. Pharmacogn Rev 2010;4:209-14.
10. Haque MM, Alsareii SA. A review of the therapeutic effects of using miswak (Salvadora persica) on oral health. Saudi Med J 2015;36:530-43.
11. Aumeeruddy MZ, Zengin G, Mahomoodally MF. A review of the traditional and modern uses of Salvadora persica L. (Miswak): Toothbrush tree of prophet Muhammad. J Ethnopharmacol 2018;213:409-44.
12. Al-Ayed MS, Asaad AM, Qureshi MA, Attia HG, AlMarrani AH. Antibacterial activity of Salvadora persica L. (Miswak) extracts against multidrug resistant bacterial clinical isolates. Evid Based Complement Alternat Med 2016;2016:7083964.
13. Toyang NJ, Wanyama J, Nuwanyakpa M, Django S. Ethnoveterinary Medicine. A Practical Approach to the Treatment of Cattle Diseases in sub-Saharan Africa. Wageningen: Agromisa Foundation and CTA; 2007.
14. Sher H, Nasser AM, Wijaya L. Ethnobotanical and antibacterial potential of Salvadora persica. A well known medicinal plant in Arab and Unani system of medicine. J Med Plant Res 2011;5:1224-9.
15. Halket JM, Zaikin VG. Derivatization in mass spectrometry–1. Silylation. Eur J Mass Spectrom (Chichester) 2003;9:1-21.
16. Halket JM, Waterman D, Przyborowska AM, Patel RK, Fraser PD, Bramley PM. Chemical derivatization and mass spectral libraries in metabolic profiling by GC/MS and LC/MS/MS. J Exp Bot 2005;56:219-43.
17. Gupta R, Singh RL, Dwivedi N. In vitro antioxidant activity and GC-MS analysis of the ethanolic extracts of Terminalia bellerica roxb (baheda). Int J Pharm Pharm Sci 2016;8:275-82.
18. Bader A, Guido F, Pier LC, Ivano M. The composition of the root oil of Salvadora persica L. J Essent Oil Res 2002;14:128-9.
19. Alali F, Al-Lafi T. GC-MS analysis and bioactivity testing of the volatile oil from the leaves of the toothbrush tree Salvadora persica L. Nat Prod Res 2003;17:189-94.
20. Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman & Hall; 1998.
21. Riddick JA, Burger WB. Organic solvents. In: Weiss Berger A, editor. Techniques of Chemistry. 3rd ed., Vol. 2. New York: Willey-Inter Science; 1970.
22. Vogel AI, Tatchell AR, Furnis BS. Vogel’s Textbook of Practical Organic Chemistry. 5th ed. London: Pearson Education; 1989. p. 165-8, 397-403.
23. Gudzic B, Nedeljkovic J, Dordevic S, ?omor JJ. Composition and anti-microbial activity of essential oil of hyperici herb (Hypericum perforatum L.) from Vlasina region. Phys Chem Technol 1997;1:47-51.
24. Maruzella JC, Henry PA. The antimicrobial activity of perfume oils. J Am Pharm Assoc 1958;47:471-6.
25. Bharucha FR, Rangnekar PV. Studies in free amino acids and organic acids of halophytes of Bombay. Naturwissenschaften 1957;44:469.
26. Raj K, Agrawal Y. Heavy metal contents of the leaf-galls of Salvadora persica Linn. Sci Cult 1979;45:35-6.
27. El Sayed ZA. Identification of inorganic constituents of Salvadora persica using spectroscopic techniques. Bull Natl Res Centre (Egypt) 1995;20:163-70.
28. Bahabri F, Jeddah. Application of spectroscopic techniques for the identification of organic and inorganic constituents of Salvadora persica from Saudi Arabia. Physica A 2000;276:346-51.
29. Kamel MS, Ohtani K, Assaf MH, Kasai R, El-Shanawani MA, Yamasaki K, et al. Lignan glycosides from stems of Salvadora persica. Phytochemistry 1992;31:2469-71.
30. Malik S, Ahmad SS, Haider SI, Muzaffar A. Salvadoricine – A new indole alkaloid from the leaves of Salvadora persica. Tetrahedron Lett 1987;28:163-4.
31. Khan SA, Qureshi MI, Bhatty MK. Fatty acids of indigenous resources for possible industrial applications. IV. Species of Salvadoraceae family. Pak J Sci Ind Res 1972;15:402-4.
32. Hosamani KM, Pattanashettar RS. Salvadora persica seed oil: A rich source of oil and minor source of cyclopropenoid fatty acids. J Med Aromat Plant Sci 2002;24:713-5.
33. Galletti GC, Chiavari G, Kahie YD. Pyrolysis/gas chromatography/ion-trap mass spectrometry of the ‘tooth brush’ tree (Salvadora persica L.). Rapid Commun Mass Sp 1993;7:651-5.
34. Khalil AT. Benzylamides from Salvadora persica. Arch Pharm Res 2006;29:952-6.
35. Khan I, Chauhan N, Chauhan PK, Khan MA. Evaluation of phytoconstituents, and antimicrobial and antioxidant activity of Juglans regia from Kashmir region. Asian J Pharm Clin Res 2019;12:89-92.
36. Alali F, Hudaib M, Aburjai T, Khairallah K, Al-Hadidi N. GC-MS analysis and antimicrobial activity of the essential oil from the stem of the Jordanian toothbrush tree Salvadora persica. Pharm Biol (Lisse, Netherlands) 2004;42:577-80.
37. Almas K, Skaug N, Ahmad I. An in vitro antimicrobial comparison of miswak extract with commercially available non-alcohol mouthrinses. Int J Dent Hyg 2005;3:18-24.
38. Darmani H, Nusayr T, Al-Hiyasat AS. Effects of extracts of miswak and derum on proliferation of Balb/C 3T3 fibroblasts and viability of cariogenic bacteria. Int J Dent Hyg 2006;4:62-6.
39. Siddiqui S, Khan SS, Ansari AH. Antimicrobial activity of Salvadora persica. J Ultra Chem 2006;2:193-6.
40. Abhary M, Al-Hazmi AA. Antibacterial activity of Miswak (Salvadora persica L.) extracts on oral hygiene. J Taibah Univ Sci 2016;10:513-20.
41. Sofrata A, Santangelo EM, Azeem M, Borg-Karlson AK, Gustafsson A, Pütsep K. Benzyl isothiocyanate, a major component from the roots of Salvadora persica is highly active against gram-negative bacteria. PLoS One 2011;6:e23045.
42. Albabtain R, Azeem M, Wondimu Z, Lindberg T, Borg-Karlson AK, Gustafsson A. Investigations of a possible chemical effect of Salvadora persica chewing sticks. Evid Based Complement Alternat Med 2017;2017:2576548.
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How to Cite
PRABHAKARAN K, JOHN BRITTO S, and PREETHI J. “IDENTIFICATION OF ETHNOMEDICINAL COMPOUNDS AND ANTIMICROBIAL STUDIES OF SALVADORA PERSICA L. (SALVADORACEAE)”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 4, Apr. 2019, pp. 183-8, doi:10.22159/ajpcr.2019.v12i4.31256.
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