PRODUCTION OF BETALAINS FROM HAIRY ROOT CULTURE OF BETA VULGARIS AND ITS USE IN PARACETAMOL SYRUP AS A NATURAL COLOURANT
Keywords:Beta vulgaris, Betalains, Agrobacterium Rhizogens, Paracetamol syrup
Objective: The present study was aimed to extract betalains from hairy root culture of Beta vulgaris and its use in pharmaceutical formulations as a colorant.
Methods: Hairy roots were initiated using different strains of Agrobacterium rhizogenes such as A.2/83, A.20/83, A.4, and LMG 150; LMG 150 was found to initiate a large number of hairy roots, and betalain content was estimated. Paracetamol syrup was prepared using extracted betalains as a colorant at different concentrations of 10 and 30 mg/150 ml. Stability studies were carried out at a different temperatures such as 25°C, 30°C, and 40°C) and light (dark, 1000 and 2000 lux) for 45 days.
Results: In case of a concentration of 10 mg/150 ml syrup, the effects of temperature such as 25°C, 30°C, and 40°C and dark condition on the degradation of betalains were found to be 48%, 88%, and 100% in 45 days, respectively. The effects of temperature such as 25°C, 30°C, and 40°C at light 1000 lux on degradations of betalains were found to be 81% and 98% at 25°C and 30°C in 45 days, respectively, and 100% at 40°C in 30 days and at 2000 lux were found to be 100% at 25°C and 30°C in 30 days and 100% at 40°C in 10 days. The similar levels of degradation rate were observed with a concentration of 30 mg/150 ml.
Conclusion: Experimental data demonstrated that formulation with betalains exhibited better stability at the dark condition and lower temperature.
Shanthanakrishan. Food colors and their future. Ind Fd Pac 1981. p. 79 87.
Hempel J, Bohm J. Betaxanthin pattern of hairy roots from Beta vulgaris var. Lutea and its alteration by feeding of amino acids. Phytochemistry 1997;44:847-52.
Rudrappa T, Neelwarne B, Kumar V, Lakshmanan V, Aswathanarayana RG. Peroxidase production from hairy root cultures of red beet (Beta vulgaris). Electron J Biotech 2005;8:243-7.
Rink E, Bohm H. Changed betaxanthin pattern in violet flowers of Portulaca glandiflora after the feeding of dopa. Phytochemistry 1985;24:1475-7.
Bhagyalakshmi N, Ravishankar GA. Natural compounds from hairy roots. In: Role of Biotechnology in Medicinal and Aromatic Plants. Vol. 1. Hyderabad: Ukaaz Publications; 1997. p. 166-82.
Kukreja AK, Zehra M, Sultana A, Banarjee S. Hairy roots and medicinal plants: Potential and prospects. In: Role of Biotechnology in Medicinal and Aromatic Plants. Vol. 1. Hyderabad: Ukaaz Publications; 1996. p. 74-89.
Pszczola DE. Natural colors: Pigments of imagination. Food Tech 1998;52:70-81.
Shimomura K, Sudo H, Saga H, Kamada H. Shikonin production and secretion by hairy root cultures of Lithospermum erythrorhizon. Plant Cell Rep 1991;10:282-5.
EL-Geng Aihi SE, Hamed MA, Doha H, Abdel AB, Mossa TH. Flavonoids from sugar beet serves as hepatoprotective agent. Int J Pharm Pharm Sci 2016;8:281-6.
Warrier PK, Nambier VP, Ramamutthy C. Indian Medicinal Plants. Vol. 1. Hyderabad: Orient Longman Limited.; 1994. p. 165-6.
Yoshikawa M, Murakami T, Kadoya M, Matsuda H, Muraoka O, Yamahara J, et al. Medicinal foodstuff. III. Sugar beet. (1): Hypoglycemic oleanolic acid oligoglycosides, betavulgarosides I, II, III, and IV, from the root of Beta vulgaris L. (Chenopodiaceae). Chem Pharm Bull (Tokyo) 1996;44:1212-7.
Suganyadevi P, Saravanakumar M, Aravinthan KM, Arunkumar A, Kavitha Krishna R, Karthikeyani S. Extraction of betacyanin from red beet roots (Beta vulgaris L) and to evaluate its antioxidant potential. J Pharm Res 2010;3:2693-6.
Levy LW, Rivadeneria DM. Anatto. In: Natural Food Colorants Science and Technology. 1st ed. New York: Marcel Dekker Inc.; 2000. p. 132-4.
Nilson T. Studies into the pigments of beet root. LantbrukHogsk Ann 1970;36:179.
Kohli DP. Liquid orals. In: Drug Formulation Manual. 3rd ed. New Delhi: Eastern Publishers; 1998. p. 437-8.
Patil N, Patar A. Extraction, Stability and separation of anthocyanins of Ixora coccinea Linn. Int J Pharm Pharm Sci 2015;7:198-202.
Sutriyo, Iswandana R, Nurwidiya E. Effect of variation of Beta vulgaris extracts on masking the bitter taste of Momordica chirantia extract syrup. Int J Appl Pharm 2018;10:159-64.
Bias HP, Madhusudhan R, Bhagyalakshmi N, Rajashekaran T, Ramesh BS, Ravishankar GA. Influence of polyamines on growth and formation of secondary metabolites in hairy root cultures of Beta vulgaris and Tagets patula. Acta Physiol Plant 2000;22:151-8.
Weller TA, Lasure LL. Betalains in beet root tissue culture. J Food Sci 1981;47:62.
Von Elbe JH, Goldman IL. The betalains. In: Natural Food Colorants Science and Technology. Basic Symposium Series. Boca Raton, FL: CRC Press; 1975. p. 11-29.
Von Elbe JH, Young Maing IL, Amundson CH. Color stability of Betacyanin. J Food Sci 1974;39:334-7.
How to Cite
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.