GENOPROTECTIVE EFFECTS OF ETHANOLIC STEM EXTRACTS OF TINOSPORA CORDIFOLIA ON SODIUM ARSENITE-INDUCED DNA DAMAGE IN SWISS MICE LYMPHOCYTES BY COMET ASSAY
Keywords:Genoprotective, Genotoxicity, DNA migration, Oxidative stress
Objective: The study was aimed to evaluate the DNA protective effects of ethanolic extracts of Tinospora cordifolia stem on Swiss albino mice lymphocytes against the sodium arsenite-induced genotoxicity.
Methods: In this experiment, 25 animals of nearly the same age were equally divided. Group 1 (control) fed on tap water while rest four (2, 3, 4, and 5) groups were treated with 4 mg/kg body weight, per orally sodium arsenite for 12 weeks. Sodium arsenite pretreated groups (4 and 5) were followed by administration of 80 mg/kg body weight, per orally T. cordifolia extracts for the next 12 weeks. Animals were sacrificed at the 8th week and 12th week, respectively, for the assessment of sodium arsenite-induced genotoxicity and comparative genoprotective effects of experimental plant extracts. The extent of DNA migration is directly proportional to the magnitude of DNA damage. Percentage tail DNA content and olive tail moment parameters were used in the comet to relate DNA damage.
Results: The findings suggested that the ethanolic stem extracts of T. cordifolia significantly inhibited the sodium arsenite-induced oxidative genotoxicity. The phytoconstituents of T. cordifolia shown to retard genetic damage associated with arsenic exposure.
Conclusion: T. cordifolia may be used as a preventive herbal preparation against chemical or arsenical toxicity.
Collins AR. The comet assay: A heavenly method. Mutagenesis 2015;30:1-4.
Azqueta A, Slyskova J, Langie SA, Gaivão IO, Collins A. Comet assay to measure DNA repair: Approach and applications. Front Genet 2014;5:1-8.
Maurici D, Aardema M, Corvi R, Kleber M, Krul C, Laurent C, et al. Genotoxicty and mutagenicity. Altern Lab Anim 2005;1:117-30.
Enciso M, Sarasa J, Agarwal A, Fernández JL, Gosálvez J. A two-tailed comet assay for assessing DNA damage in spermatozoa. Reprod Biomed Online 2009;18:609-16.
Lapuente J, Lourenço J, Mendo SA, Borràs M, Martins MG, Costa PM, et al. The comet assay and its applications in the field of ecotoxicology: A mature tool that continues to expand its perspectives. Front Genet 2015;6:1-20.
Collins AR. The comet assay for DNA damage and repair: Principle, applications, and limitations. Mol Biotechnol 2004;26:249-61.
Liao W, McNutt MA, Zhu WG. The comet assay: A sensitive method for detecting DNA damage in individual cells. Methods 2009;48:46-53.
Kapaj S, Peterson H, Liber K, Bhattacharya P. Human health effects from chronic arsenic poisoning-a review. J Environ Sci Health A Tox Hazard Subst Environ Eng 2006;41:2399-428.
Cohen SM, Arnold LL, Eldan M, Lewis AS, Beck BD. Methylated arsenicals: The implications of metabolism and carcinogenicity studies in rodents to human risk assessment. Crit Rev Toxicol 2006;36:99-133.
Jomova K, Jenisova Z, Feszterova M, Baros S, Liska J, Hudecova D, et al. Arsenic: Toxicity, oxidative stress and human disease. J Appl Toxicol 2011;31:95-107.
Kitchin KT. Recent advances in arsenic carcinogenesis: Modes of action, animal model systems and methylated arsenic metabolites. Toxicol Appl Pharmacol 2001;172:249-61.
Kligerman AD, Tennant AH. Insights into the carcinogenic mode of action of arsenic. Toxicol Appl Pharmacol 2007;222:281-8.
Majumdar KK. Arsenicosis and cancer: A perspective. Discov Biol 2015;1:1-4.
Faita F, Cori L, Bianchi F, Andreassi MG. Arsenic-induced genotoxicity and genetic susceptibility to arsenic-related pathologies. Int J Environ Res Public Health 2013;10:1527-46.
Ferrario D, Collotta A, Carfi M, Bowe G, Vahter M, Hartung T, et al. Arsenic induces telomerase expression and maintains telomere length in human cord blood cells. Toxicology 2009;260:132-41.
Flora SJ, Bhaduaria S, Kannan GM, Singh N. Arsenic induced oxidative stress and role of antioxidant supplementation during chelation: A review. J Environ Biol 2007;28:333-47.
Mourón SA, Grillo CA, Dulout FN, Golijow CD. Induction of DNA strand breaks, DNA-protein cross links and sister chromatid exchanges by arsenite in a human lung cell line. Toxicol In Vitro 2006;20:279-85.
Bustaffa E, Stoccoro A, Bianchi F, Migliore L. Genotoxic and epigenetic mechanism in arsenic carcinogenicity. Arch Toxicol 2014;88:1043-67.
Kousar S, Javed M. Assessment of DNA damage in peripheral blood erythrocytes of fish exposed to arsenic under laboratory conditions. Int J Curr Microbial Appl Sci 2015;3:877-88.
Mittal J, Sharma MM, Batra A. T. cordifolia: A multipurpose medicinal plant-a review. J Med Plants Stud 2014;2:32-47.
Singh SS, Pandey SC, Srivastava S, Gupta VS, Patro B, Ghosh AC. Chemistry and medicinal properties of Tinopora cordifolia (Guduchi). Indian J Pharmacol 2003;35:83-91.
Sudhakar C, Joshi LK, Sehgal A. Assessment of antioxidant and anti-lipid peroxidation capability of Guduchi (T. cordifolia). Res J Pharm Biol Chem Sci 2015;6:458-63.
Bala M, Gupta GL. Hepato and nephro-protective effects of Tinospora cordifolia against sodium nitrite induced oxidative stress. Indo Am J Pharm Res 2016;6:4102-10.
Verma R, Chaudhary HS, Agrawal RC. Evaluation of anticarcinogenic and antimutagenic effect of Tinospora cordifolia in experimental animals. J Chem Pharm Res 2011;3:877-81.
Ahmed MK, Hossain MA, Arif M, Perwin E, Khan MS, Islam MM. Assessing the genotoxic potential of arsenic in tilapia using alkaline comet assay and micronucleus test. Chemosphere 2011;84:143-9.
Behravan J, Mosafa F, Soudmand N, Taghiabadi E, Razavi BM, Karimi G. Protective effects of aqueous and ethanolic extracts of Portulaca oleracea L. Aerial parts on H2O2- induced DNA damage in lymphocytes by comet assay. J Acupunct Meridian Stud 2011;4:193-7.
Mozaffarich M, Schoetzau A, Sauter M, Grieshaber M, Orgul S, Chaja OG, et al. Comet assay analysis of single stranded DNA breaks in circulating leucocytes of glaucoma patients. Mol Vis 2008;14:1584-8.
Kumaravel TS, Vilhar B, Faux SP, Jha AN. Comet assay measurements: A perspective. Cell Biol Toxicol 2009;25:53-64.
Liu SX, Athar M, Lippai I, Waldren C, Hei TK. Induction of oxygen radicals by arsenic: Implications for mechanism of genotoxicity. ProcNatl Acad Sci USA 2001;98:1643-8.
Wang TS, Huang H. Active oxygen species are arsenic affect oxidative system in pig 733 involved in the induction of micronuclei in XRS- 5 cells. Mutagenesis 1994;9:253-7.
Balakrishnan BB, Krishnasamy K. Evaluation of free radical screening and antioxidants potential of Moringa concanensis nimmo- a medicinal plant used in Indian traditional medication system. Int J Pharm Pharm Sci 2018;10:91-7.
Mandal BK, Suzuki KT. Arsenic round the world: A review. Talanta 2002;58:201-35.
Zhang Z, Wang X, Cheng S, Sun L, Son YO, Yao H, et al. Reactive oxygen species mediate arsenic induced cell transformation and tumorigenesis through Wnt/beta -catenin pathway in human colorectal adenocarcinoma DLD1 cells. Toxicol Appl Pharmacol 2011;256:114-21.
Singh RP, Banerjee S, Kumar PV, Raveesha KA, Rao AR. Tinospora cordifolia induces enzymes of carcinogen/drug metabolism and antioxidant system and inhibits lipid peroxidation in mice. Phytomedicine 2006;13:74-84.
Nwachukwu CU, Umeh CN, Kalu IG, Slyvester O, Magnus CN. Identification and traditional uses of some common medicinal plants in Ezinihitte Mbaise LGA of Imo state, Nigeria. Rep Opin 2010;2:1-8.
Mohanapriya M, Ramaswamy L, Rajendran R. Health and medicinal properties of lemon (Citrus limonum). Int J Ayurvedic Herb Med 2013;3:1095-100.
Bhawya D, Anilakumar KR. In vitro antioxidant potency of Tinospora cordifolia (gulancha) in sequential extracts. Int J Pharm Biol Arch 2010;1:448-56.
Kapkoti B, Lodhiyal N, lodhiyal LS. Ethno-medicinal plants and their use by van panchayat people in Nainital of Kumaun region, Uttrakhand. Biolife 2014;2:526-32.
Jiofack T, Fokunang C, Guedje N, Kemeuze V, Fongnzossie E, Nkongmeneck BA, et al. Ethnobotanical uses of medicinal plants of two ecological regions of Cameroon. Int J Med Med Sci 2010;2:60-79.
Wink M. Modes of action of herbal medicines and plants secondary metabolites. Medicines 2015;2:251-86.
Ilaiyaraja N, Khanum F. Antioxidant potential of Tinospora cordifolia extract and their protective effect on oxidation of biomolecules. Pharmacogn J 2011;3:56-62.
Pandey P, Mehta A, Hajra S, John J, Mehta P. Antioxidant property, total phenolic content and inhibition of α-amylase activity of Ruta graveolens L. Leaves extract. J Pharmacol Res 2011;4:1735-7.
Aqil F, Ahmed I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turk J Biol 2006;30:177-83.
Srivastava P. Tinospora cordifolia (Amrita) - a miracle herb and lifeline too many diseases. Int J Med Aromat Plants 2011;1:57-61.
Mathew S, Kuttan G. Antioxidant activity of Tinospora cordifolia and its usefulness in the amelioration of cyclophosphamide induced toxicity. J Exp Clin Cancer Res 1997;16:407-11.
Goel HC, Prasad J, Singh S, Sagar RK, Agrawal PK, Bala M, et al. Radio protective potential of an herbal extract of Tinospora cordifolia. J Radiat Res 2004;45:57-64.
Panares KA, Abamo F, Billacura M. Genotoxic, anti-genotoxic and protective evaluation of Tinospora cordifolia stem by peripheral blood micronucleus assay. Sci Int 2017;29:97-101.
Kumar M, Maneemegalai S. Evaluation of larvicidal effect of Lantana camara Linn. Against mosquito species Aedes aegypti and Culex quinquefasciatus. Adv Biol Res 2008;2:39-43.
Premkumar A, Kreitman RJ, Kindler H, Willingham MC, Pastan I. Phase I study of SS1P, a recombinant anti-mesothelin immunotoxin given as a bolus I.V infusion to patients with mesothelin-expressing mesothelioma, ovarian, and pancreatic cancers. Clin Cancer 2004;13:5144-9.
Meziti A, Bouriche H, Hichem M, Kada S, Senator A, Dimertas I. Antioxidant and anti-inflammatory activities of Rubus fruticosus and Zizyphus vulgaris methanol extracts. Int J Pharm Pharm Sci 2017;9:69-76.
Bala M, Pratap K, Verma PK, Singh B, Padwad Y. Validation of ethno medicinal potential of Tinospora cordifolia for anticancer and immuno-modulatory activities and quantification of bioactive molecules by HPTLC. J Ethnopharmacol 2015;175:131-7.
Sharma U, Bala M, Kumar P, Rampal G, Kumar N, Singh B, et al. Antimutagenic extract from Tinospora cordifolia and its chemical composition. J Med Plants Res 2010;4:2488-94.
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