EVALUATION OF IN VITRO CYTOTOXIC AND ANTICANCER ACTIVITY OF HEPATOPROTECTIVE POLYHERBAL FORMULATION IN CELL LINE MODEL

  • JAYACHANDRA KUNCHA Department of Biotechnology, Periyar Maniammai Institute of Science and Technology, Thanjavur, Tamil Nadu, India.
  • THIRUGNANASAMBANTHAM P Rumi Herbals R and D Centre, Chennai, Tamil Nadu, India.
  • KUMARAN S Department of Biotechnology, Periyar Maniammai Institute of Science and Technology, Thanjavur, Tamil Nadu, India.
  • NARAYANAN N Department of Pharmaceutics, A.J College of Pharmacy, Chennai, Tamil Nadu, India,
  • SHARMILA DEVI V Department of Biochemistry, Captain Srinivasa Murthy Regional Ayurveda Drug Development Institute, Chennai, Tamil Nadu, India.

Abstract

Introduction: The use of natural products as anticancer agents has a long history that began with folklore medicine and through the years has been incorporated into traditional and allopathic medicine. Several drugs currently used are derived from medicinal plants.


Objective: The main objective of this study is to investigate the cytotoxic potential of hepatoprotective polyherbal formulation in normal and cancer cell lines.


Methods: A 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was utilized to screen the cytotoxic activity.


Results: The results revealed that the formulation does not induce much mortality in normal liver and kidney cell lines, and LC50 value of liver cell lines was found 1716.355 μg/ml and kidney cell lines 2464.910 μg/ml. The in vitro anticancer activity was performed on liver, colon, and prostate cancer cell lines, and IC50 values are found 2.077, 3.850, and 11.989 μg/ml, respectively, which show excellent anticancer activity.


Conclusion: Based on the results obtained, the hepatoprotective polyherbal formulation is safe for normal cells and cytotoxic for cancer cells. Further, identification and quantification of phytoconstituents responsible for the activity are in progress.

Keywords: Polyherbal formulation, phytochemicals, Cytotoxicity,  anticancer activity.

Author Biography

JAYACHANDRA KUNCHA, Department of Biotechnology, Periyar Maniammai Institute of Science and Technology, Thanjavur, Tamil Nadu, India.

Research associate

References

1. Ilyas U, Katare DP, Aeri V, Naseef PP. A review on hepatoprotective and immunomodulatory herbal plants. Pharmacogn Rev 2016;10:66-70.
2. Patel S, Gheewala N, Suthar A, Shah A. In-vitro cytotoxicity activity of Solanum nigrum extract against Hela cell line and Vero cell line. Int J Pharm Pharm Sci 2009;1 Suppl 1:38-46.
3. Jiménez-Medina E, Garcia-Lora A, Paco L, Algarra I, Collado A, Garrido F, et al. A new extract of the plant Calendula officinalis produces a dual in vitro effect: Cytotoxic anti-tumor activity and lymphocyte activation. BMC Cancer 2006;6:119.
4. Fotakis G, Timbrell JA. In vitro cytotoxicity assays: Comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 2006;160:171-7.
5. Stone V, Johnston H, Schins RP. Development of in vitro systems for nanotoxicology: Methodological considerations. Crit Rev Toxicol 2009;39:613-26.
6. Vijayabaskaran M, Venkateswaramurthy N, Pasha A, Babu G, Sivakumar P, Perumal P, et al. In vitro cytotoxic effect of ethanolic extract of Pseudarthria viscida Linn. Int J Pharm Pharm Sci 2010;2:93-4.
7. Sharma S, Kumar VN. In vitro cytotoxicity effect on MCF-7 cell line of co-encapsulated artesunate and curcumin liposome. Int J Pharm Pharm Sci 2017;9:123-8.
8. Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
9. Präbst K, Engelhardt H, Ringgeler S, Hübner H. Basic colorimetric proliferation assays: MTT, WST, and resazurin. Methods Mol Biol 2017;1601:1-7.
10. Tyson CA, Green CE. Cytotoxicity measures: Choices and methods in the isolated hepatocyte, In: Rauckman EG, Padilla GM, editors. Use in Toxicology and Xenobiotic Biotransformations. Orlando, FL: Academic Press; 1987. p. 119-58.
11. Boyd MR, Paull KD. Some practical considerations and applications of the national cancer institute in vitro anticancer drug discovery screen. Drug Dev Res 1995;34:91-9.
12. González LT, Minsky NW, Espinosa LE, Aranda RS, Meseguer JP, Pérez PC, et al. In vitro assessment of hepatoprotective agents against damage induced by acetaminophen and CCl4. BMC Complement Altern Med 2017;17:39.
13. Manosroi A, Akazawa H, Akihisa T, Jantrawut P, Kitdamrongtham W, Manosroi W, et al. In vitro anti-proliferative activity on colon cancer cell line (HT-29) of thai medicinal plants selected from thai/Lanna medicinal plant recipe database “MANOSROI III”. J Ethnopharmacol 2015;161:11-7.
14. Prashant A, Rangaswamy C, Yadav AK, Reddy V, Sowmya MN, Madhunapantula S, et al. In vitro anticancer activity of ethanolic extracts of Piper nigrum against colorectal carcinoma cell lines. Int J Appl Basic Med Res 2017;7:67-72.
15. Shridhar C, Ghagane A, Sridevi I, Puranik SI, Kumbar VM, Rajendra B, et al. In vitro antioxidant and anticancer activity of Leea indica leaf extracts on human prostate cancer cell lines. Integr Med Res 2017;6:79-7.
Statistics
36 Views | 16 Downloads
How to Cite
JAYACHANDRA KUNCHA, THIRUGNANASAMBANTHAM P, KUMARAN S, NARAYANAN N, and SHARMILA DEVI V. “EVALUATION OF IN VITRO CYTOTOXIC AND ANTICANCER ACTIVITY OF HEPATOPROTECTIVE POLYHERBAL FORMULATION IN CELL LINE MODEL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 4, Mar. 2019, pp. 320-5, https://innovareacademics.in/journals/index.php/ajpcr/article/view/32118.
Section
Original Article(s)