Characterization and Prevalence of Clindamycin Resistance Staphylococcus aureus from Clinical samples of National Medical College and Teaching Hospital, Nepal

AMRULLAH SHIDIKI; BIJAY RAJ PANDIT; ASHISH VYAS

doi:10.22159/ajpcr.2019.v12i5.32257

Authors

AMRULLAH SHIDIKI Department of Microbiology and Biochemistry, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.
BIJAY RAJ PANDIT Department of Microbiology and Biochemistry, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.
ASHISH VYAS Department of Microbiology and Biochemistry, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i5.32257

Keywords:

Methicillin-resistant Staphylococcus aureus, Methicillin sensitive Staphylococcus aureus, Inducible macrolide-lincosamide-streptogramin B and constitutive macrolide-lincosamide-streptogramin B, Erythromycin, Oxacillin, Clindamycin

Abstract

Objective: Clindamycin is the drug of choice for the treatment of severe form of skin, soft tissue, and blood infections caused by resistant Staphylococcus aureus in the form of methicillin-resistant S. aureus (MRSA) and erythromycin-resistant S. aureus. In this research, we determine the susceptibility pattern of isolated S. aureus strains against antibiotics and the prevalence of resistant S. aureus in the form of MRSA, inducible clindamycin-resistant S. aureus (inducible macrolide-lincosamide-streptogramin B [iMLSB]) and constitutive clindamycin-resistant S. aureus (cMLSB).

Methods: A total of 310 isolated S. aureus among 2000 different clinical samples were subjected to oxacillin (1 μg) as per the Kirby-Bauer disk diffusion method for MRSA. Clindamycin-resistant either in the form of iMLSB or cMLSB was determined through double disk diffusion method or D-test by use erythromycin (2 μg) and clindamycin (15 μg) as per the CLSI guidelines.

Results: Out of total S. aureus, MRSA and methicillin-sensitive S. aureus (MSSA) were 78.06% and 20.64%, respectively. This study showed that iMLSB and cMLSB were 34.19% and 23.22%. Both iMLSB and cMLSB were found more among MRSA than MSSA (43.80%, 26.85% and 40.62%, 10.93%), respectively.

Conclusion: This study helps for the characterization of different resistant strains of S. aureus along with the determination of the prevalence rate of these mutant forms causing nosocomial infections.

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Author Biographies

BIJAY RAJ PANDIT, Department of Microbiology and Biochemistry, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.

He help for doing reserach

ASHISH VYAS, Department of Microbiology and Biochemistry, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara, Punjab, India.

He is also support for doing work for complrting the research

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