AMELIORATIVE EFFECT OF BAUHINIA VARIEGATA ON HYPERCHOLESTEROLEMIA-INDUCED OXIDATIVE STRESS IN RATS
Keywords:Oxidative stress,, Low-density lipoprotein,, Very low-density lipoproteins,, Cardiovascular diseases
Objective: The objective of this study was to assess the effect of the methanolic extract of Bauhinia variegata (L.) stem bark in albino rats.
Methods: The coarsely powdered stem bark of B. variegata (L.) was extracted with methanol (BVMtE) and was finally filtered. The experimental animals were divided into five groups for the duration of 60 days: control group (Group I); high cholesterol diet (HCD)-fed group (Group II); BVME-treated (100 mg/g b. wt./day) HCD group (Group III); BVME-treated (200 mg/g b. wt./day) HCD group (Group IV); and atorvastatin-treated (40 μg/g b. wt./day) HCD group (Group V). Animals were sacrificed and blood and tissues were removed for biochemical and histological analysis.
Results: Oral BVMtE administration to high cholesterol diet (HCD)-fed rats significantly normalized the serum total cholesterol, low-density lipoprotein and very low-density lipoprotein cholesterol, triglycerides (TGs), phospholipid, aspartate and alanine transaminase, and alkaline and acid phosphatase. Reduced glutathione, superoxide dismutase, as well as atherogenic index of BVMtE-treated HCD rats showed significant normalcy as compared to HCD-fed rats. The histoarchitecture of heart and aorta of BVMtE-treated HCD-fed rats depicted marked normalcy as compared to HCD rats. Phytochemical analysis showed that plant is rich in active constituents, i.e., flavonoids, steroids, terpenoids, β-sitosterol, kaempferol-3-glucoside, and tannins.
Conclusions: B. variegata has proved lipid lowering as well as antioxidant activity in high cholesterol diet-fed animals.
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