EVALUATION OF ANTIPROLIFERATIVE ACTIVITY OF SIDDHA ANTI-PSORIATIC FORMULATION PANCHAMUGA CHENDHURAM USING CULTURED HUMAN KERATINOCYTE CELL LINES
Objectives: This study was aimed at scientifically evaluating the in vitro antipsoriatic activity of Siddha drug Panchamuga Chendhuram (PMC) in human keratinocyte (HacaT) cell lines.
Methods: The Siddha drug PMC tested for antipsoriatic activity on HacaT cell lines was morphologically examined by phase contrast microscopy, and the cell viability was determined by 3- (4, 5 dimethyl thiazole-2 yl) -2.5-diphenyl tetrazolium bromide assay. About 100 μl of different concentrations (2, 6, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 μg/ml) of the test samples were prepared in the cell culture medium and incubated for 24 h and 48 h to determine the viable cells.
Results: The results revealed that Siddha drug PMC showed hopeful antiproliferative activity. In vitro studies showed that after 24 h and 48 h incubation, the inhibitory concentration 50 (IC50) values of PMC (IC50 20 μg/ml) were 72.08±27.56 μg/ml and 43.91±17.71 μg/ml, respectively, as compared with Asiaticoside as a positive control with an IC50 value of 20.13 μg/ml.
Conclusion: Thus, this study provides scientific evidence about the efficacy of the Siddha drug PMC against the HacaT cell lines confirming its traditional use in psoriasis treatment and also emphasizes the need for antipsoriatic evaluation in animal models.
2. Glickman FS. Lepra, psora, psoriasis. J Am Acad Dermatol 1986;14:863-6.
3. Rajan AK, Jeyamani SV, Kaviya U, Indumathi S, Divya R. Case study on beta blockers induced psoriasis. Int J Pharm Pharm Sci 2019;11:112-5.
4. Kumar S, Nayak CS, Padhi T, Rao G, Rao A, Sharma VK, et al. Epidemiological pattern of psoriasis, vitiligo and atopic dermatitis in India: Hospital-based point prevalence. Indian Dermatol Online J 2014;5:S6-8.
5. Soliman GM, Osman SK, Hamdan AM. Preparation and evaluation of anthralin biodegradable nanoparticles as a potential delivery system for the treatment of psoriasis. Int J Pharm Pharm Sci 2015;17:36-40.
6. NPF. The Immune System and Psoriatic Diseases. Portland: National Psoriasis Foundation; 2018.
7. Zahir HZ, Kumaresa S. GC-MS analysis and antibacterial evaluation of Acalypha indica. Asian J Plant Sci Res 2013;6:46-9.
8. Walter TM, Priya TS, Paargavi AS, Devi NP, Thanalakshmi S. A review Of herbs to treat skin disorders in traditional siddha medicine. Res Rev 2014;2:7-14.
9. Muthaliar M. Siddha Materia Medica (Vegetable section). Vol. 1. Chennai: Tamilnadu Siddha Medical Council; 1988.
10. Raja Mudhaliyar V, Vellaka P. Pulippani Vaidyam-500. Chennai: Rathnanaicker and Sons; 2009.
11. Tse WP, Che CT, Liu K, Lin ZX. Evaluation of the anti-proliferative properties of selected psoriasis-treating Chinese medicines on cultured HaCaT cells. J Ethnopharmacol 2006;108:133-41.
12. Thiyagarajan R. Gunapaadam Dhadhu Seeva Vaguppu. Chennai: The Tamil Nadu Siddha Medical Council; 2009.
13. Vijayalakshmi A, Ravichandiran V, Malarkodi V, Nirmala S, Jayakumari S. Screening of flavonoid “quercetin” from the rhizome of Smilax china Linn. For anti-psoriatic activity. Asian Pac J Trop Biomed 2012;2:269-75.
14. Saelee C, Thongrakard V, Tencomnao T. Effects of thai medicinal herb extracts with anti-psoriatic activity on the expression on NF-?B signaling biomarkers in HaCaT keratinocytes. Molecules 2011;16:3908-32.
15. Alex R, Ilango K. In vitro cytotoxic activity of isolated compounds from viburnum punctatum buch-ham ex d. Don. Int J Curr Pharm Res 2017;9:85-7.
16. Athmakur H, Kondapi AK. Carmustine loaded lactoferrin nanoparticles demonstrates an enhanced antiproliferative activity against glioblastoma. Int J Appl Pharm In Vitro 2018;10:234-41.
17. Meeuwis KA, de Hullu JA, Massuger LF, van de Kerkhof PC, van Rossum MM. Genital psoriasis: A systematic literature review on this hidden skin disease. Acta Derm Venereol 2011;91:5-11.
18. Krueger G, Koo J, Lebwohl M, Menter A, Stern RS, Rolstad T, et al. The impact of psoriasis on quality of life: Results of a 1998 national psoriasis foundation patient-membership survey. Arch Dermatol 2001;137:280-4.
19. De Korte J, Sprangers MA, Mombers FM, Bos JD. Quality of life in patients with psoriasis: A systematic literature review. J Investig Dermatol Symp Proc 2004;9:140-7.
20. Khazaei S, Esa NM, Ramachandran V, Hamid RA, Pandurangan AK, Etemad A, et al. In vitro antiproliferative and apoptosis inducing effect of Allium atroviolaceum bulb extract on breast, cervical, and liver cancer cells. Front Pharmacol 2017;8:5.
21. Rajalakshmi S, Musthafa MM, Isai M, Sathiyarajeswaran P. A comparative study to evaluate the anti-cancer activity of siddha drugs “veera rasa padhangam” and “panchamuga chendhuram” with the standard drug taxol. Int J Curr Res Chem Pharm Sci 2017;4:29-33.
22. Pol A, Bergers M, Schalkwijk J. Comparison of antiproliferative effects of experimental and established antipsoriatic drugs on human keratinocytes, using a simple 96-well-plate assay. In Vitro Cell Dev Biol Anim 2003;39:36-42.
23. Banala RR, Vemuri SK, Reddy AV, Subbaiah GP. Aqueous extract of Acalypha indica leaves for the treatment of psoriasis: In-vitro studies. Int J Bioassays 2017;6:5360-4.
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