CHEMICAL ANALYSIS OF LITSEA CUBEBA ESSENTIAL OIL HARVESTED FROM ARUNACHAL PRADESH, INDIA AND ESTIMATION OF MOSQUITO REPELLENT ACTIVITY WITH REFERENCE TO NETWORK ANALYSIS

CHEMICAL ANALYSIS MOSQUITO REPELLENT ACTIVITY LITSEA CUBEBA ESSENTIAL OIL ARUNACHAL PRADESH

  • NAYANMONI BORUAH Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India.
  • SUDARSHANA BORAH Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India.
  • PRIYANKA SARKAR Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, India.
  • HEMANTA KUMAR SHARMA Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India. https://orcid.org/0000-0003-2632-4903

Abstract

Objective: Litsea cubeba is a medicinal plant indigenous to Southeastern Asia. The fruit of the plant is rich in essential oil. A study has been carried out to investigate on the chemical composition of essential oil of the plant harvested from Arunachal Pradesh and to find out its mosquito repellent activity.


Methods: The essential oil has been isolated from the fresh fruits by hydrodistillation and analyzed by gas chromatography–mass spectrometry. Compound-compound network analysis was carried out in SPSS software (IBM SPSS Statistics 20) followed by the construction of the network using Cytoscape 3.5.0. Mosquito repellent study was carried out using standard procedure.


Results: Citronellal (17.32%), citronellol (13.85%), nimbiol (9.35%), and myrcenol (7.40%) were the major constituents of the essential oil and the essential oil illustrated 3 h mosquito repellent activity against Aedes aegypti mosquitoes.


Conclusion: From this investigation, a significant difference could be observed in the essential oil composition from the previously reported data of other regional areas of Asia, and furthermore, the essential oil possesses significant mosquito repellent activity.

Keywords: Essential oil, Gas chromatography, Mass spectrometry, Aedes aegypti, Mosquito repellent

Author Biographies

NAYANMONI BORUAH, Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India.

Pre Ph. D. Scholar, Division of Pharmaceutics, Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh 786004, India

SUDARSHANA BORAH, Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India.

Ph. D. Research Scholar, Department of Pharmaceutical Sciences, Dibrugarh University

PRIYANKA SARKAR, Department of Bioengineering and Technology, Gauhati University, Guwahati, Assam, India.

Ph. D. Student, Department of Bioengineering and Technology, Gauhati University, Guwahati, India

HEMANTA KUMAR SHARMA, Department of Pharmaceutical Sciences, Dibrugarh University, Dibrugarh, Assam, India.

Associate Professor, Division of Pharmaceutics, Department of Pharmaceutical Sciences, Dibrugarh University

References

1. Chaliha B, Lahkar L, Doley A, Kotoky R, Saikia SP, Nath SC, et al. Screening of some lesser known tree-borne oilseed plants from North East India for their oil content and major fatty acid components. Prostaglandins Leukot Essent Fatty Acids 2017;126:9-19.
2. Bhuinya T, Singh P, Mukherjee SK. Litsea cubeba medicinal values brief summary. J Trop Med Plants 2010;11:179-83.
3. Bhuinya T, Singh P, Mukherjee SK. Traditional uses of the Indian species of Litsea lam. Phytotaxonomy 2010;10:45-6.
4. Chen HC, Chang WT, Hseu YC, Chen HY, Chuang C, Lin CC, et al. Immunosuppressive effect of Litsea cubeba L. Essential oil on dendritic cell and contact hypersensitivity responses. Int J Mol Sci 2016;17:13-9.
5. Kalita N, Kalita MC, Banerjee M. Reactive oxygen species generation in the antibacterial activity of Litsea salicifolia Leaf extract. Int J Pharm Pharm Sci 2016;8:189-93.
6. Anderson C, Lis-Balchin M, Kirk-Smith M. Evaluation of massage with essential oils on childhood atopic eczema. Phyther Res 2000;14:452-6.
7. Wang CY. The active principles of Litsea cubeba in the treatment of coronary heart disease. Zhong Yao Tong Bao 1985;10:30-2.
8. Gogoi R, Loying R, Sarma N, Mundaa S, Pandeya SK, Lala M. A comparative study on antioxidant, anti-inflammatory, genotoxicity, antimicrobial activities and chemical composition of fruit and leaf essential oils of Litsea cubeba Pers from North East India. Ind Crops Prod 2018;125:131-9.
9. Liao PC, Yang TS, Chou JC, Chen J, Lee SC, Kuo YH, et al. Anti-inflammatory activity of neral and geranial isolated from fruits of Litsea cubeba Lour. J Funct Foods 2015;19:248-58.
10. Ka SM, Lin JC, Lin TJ, Liu FC, Chao LK, Ho CL, et al. Citral alleviates an accelerated and severe lupus nephritis model by inhibiting the activation signal of NLRP3 inflammasome and enhancing nrf2 activation. Arthritis Res Ther 2015;17:331.
11. Zhang HJ, Zheng LH, Zhao K, Chen Y, Yi Z. Insecticidal activities of constituents of Litsea cubeba fruit extracts effective against the maize weevil (Coleoptera: Curculionidae). J Insect Sci 2017;17: ???.
12. Seal S, Chatterjee P, Bhattacharya S, Pal D, Dasgupta S, Kundu R, et al. Vapor of volatile oils from Litsea cubeba seed induces apoptosis and causes cell cycle arrest in lung cancer cells. PLoS One 2012;7:e47014.
13. Ho CL, Jie-Pinge O, Liu YC, Hung CP, Tsai MC, Liao PC, et al. Compositions and in vitro anticancer activities of the leaf and fruit oils of Litsea cubeba from Taiwan. Nat Prod Commun 2010;5:617-20.
14. Majumder M, Sharma HK, Zaman K, Lyngdoh W. Evaluation of physico chemical properties and antibacterial activity of the essential oil obtained from the fruits of Zanthoxyllum acanthopodium DC. Collected from Meghalaya, India. Int J Pharm Pharm Sci 2014;6:543-6.
15. Li Y, Kong W, Li M, Liu H, Zhao X, Yang S, et al. Litsea cubeba essential oil as the potential natural fumigant: Inhibition of Aspergillus flavus and AFB1 production in licorice. Ind Crops Prod 2016;80:186-93.
16. Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, et al. Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res 2003;13:2498-504.
17. World Health Organization. Guidelines for Efficacy Testing of Mosquito Repellents for Human Skin. Geneva: World Health Organization; 2009.
18. Si L, Chen Y, Han X, Zhan Z, Tian S, Cui Q, et al. Chemical composition of essential oils of Litsea cubeba harvested from its distribution areas in China. Molecules 2012;17:7057-66.
19. Wang H, Liu Y. Chemical composition and antibacterial activity of essential oils from different parts of Litsea cubeba. Chem Biodivers 2010;7:229-35.
20. Yang Y, Jiang J, Qimei L, Yan X, Zhao J, Yuan H, et al. The fungicidal terpenoids and essential oil from Litsea cubeba in tibet. Molecules 2010;15:7075-82.
21. Yang K, Wang CF, You CX, Geng ZF, Sun RQ, Guo SS, et al. Bioactivity of essential oil of Litsea cubeba from China and its main compounds against two stored product insects. J Asia Pac Entomol 2014;17:459-66.
Statistics
180 Views | 53 Downloads
How to Cite
NAYANMONI BORUAH, SUDARSHANA BORAH, PRIYANKA SARKAR, and HEMANTA KUMAR SHARMA. “CHEMICAL ANALYSIS OF LITSEA CUBEBA ESSENTIAL OIL HARVESTED FROM ARUNACHAL PRADESH, INDIA AND ESTIMATION OF MOSQUITO REPELLENT ACTIVITY WITH REFERENCE TO NETWORK ANALYSIS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 5, Apr. 2019, pp. 241-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/32672.
Section
Original Article(s)