• SASIKALA S Department of Biochemistry, DR. N. G. P. Arts and Science College, Coimbatore, Tamil Nadu, India.
  • KANNIKAPARAMESWARI N Department of Biochemistry, DR. N. G. P. Arts and Science College, Coimbatore, Tamil Nadu, India.


Objective: In the present study, acute toxicity studies were evaluated on scientifically developed nephroprotective polyherbal formulation. Polyherbal formulation consists of ethanolic extracts of dried plants of Boerhavia diffusa (BD) and Achyranthes aspera (AA).

Materials and Methods: In an acute toxicity study, polyherbal formulation was administered once orally at doses ranging from 250 mg/kg to 2000 mg/kg for 15 days. Body weight and food consumption were noted during the study period. Behavioral pattern and toxic effects were monitored in each group of animals daily. At the end of the study, blood was withdrawn for hematology and biochemical estimations. The animals were then sacrificed, and the liver, kidney, heart, and brain were dissected out which were observed for any gross morphological changes. The weights of organs were also noted.

Results: The results showed no changes in body weight, organ weight, food intake, hematological parameters, and liver function test when compared with control. The organs did not show any evidence of gross morphological changes.

Conclusion: It is concluded that developed polyherbal formulation, at a dose of 2000 mg/kg, is safe for long-term treatment of kidney disorders

Keywords: Polyherbal formulation,, Nephroprotective,, Acute toxicity,, Hematology,, Biochemistry,, Boerhavia diffusa and Achranthes aspera.


1. Dhivya JV, Santhy KS. Demystifying the ethnomedicinal plant morinda pubescens with ethnopharmacological, phytochemical, and pharmacotoxicological evidence. J Crit Rev 2018;5:1-6.
2. Subramanian K, Sankaramourthy D, Gunasekaran M. Toxicity studies related to medicinal plants. In: Natural Products and Drug Discovery. USA: Elsevier; 2018. p. 491-505.
3. Gautam MK, Goel RK. Toxicological study of Ocimum sanctum Linn leaves: Hematological, biochemical and histopathological studies. J Toxicol 2014;2014:9.
4. Nirmala RV, Abinaya R. An effect of cardioprotective activity in various medicinal plants a review. Int J Curr Pharm Sci 2019;11:1-6.
5. Talele BD, Mahajan RT, Chopda MZ, Nemade NV. Nephroprotective plants: A review. Int J Pharm Pharm Sci 2012;4:8-16.
6. Maurya H, Kumar T. Formulation, standardization, and evaluation of polyherbal dispersible tablet. Int J Appl Pharm 2019;11:158-67.
7. Pareta SK, Patra KC, Harwansh R, Kumar M, Meena KP. Protective effects of Boerhaavia diffusa against acetaminophen-induced nephrotoxicity in rats. Pharmacologyonline 2011;2:698-706.
8. Sharma VE, Chaudhary UR. An overview of indigenous knowledge of Achyranthes aspera. J Crit Rev 2015;2:7-19.
9. Santhi K, Sengottuvel R. Qualitative and quantitative phytochemical analysis of moringa concanensis nimmo. Int J Curr Microbiol Appl Sci 2016;5:633-40.
10. Organisation for Economic Co-operation and Development. Test No. 420: Acute Oral Toxicity-Fixed Dose Procedure. Paris: OECD Publishing; 2002.
11. Sood R. Textbook of Medical Laboratory Technology. 1st ed. New Delhi: Jaypee Brothers Medical Publishers (p) Ltd.; 2007. p. 204-21.
12. John MB. Laboratory Medicine Hematology. St. Louis; CV Mosby. Co.; 1972.
13. Ghai CL. A textbook of Practical Physiology. New Delhi: JP Medical Ltd.; 2012. p. 119.
14. King J. The hydrolase and alkaline phosphatase. In: Practical Clinical Enzymology. London: Nostrand Co. Ltd.; 1965. p. 191.
15. Slot C. Plasma creatinine determination. A new and specific jaffe reaction method. Scand J Clin Lab Invest 1965;17:381-7.
16. Abdulwahid SJ, Goh YM, Ebrahimi M, Mohtarrudin N, Hashim ZB. Sub-acute oral toxicity profiling of the methanolic leaf extract of clinacanthus nutans in male and female obese mice. Int J Pharm Pharm Sci 2018;10:25-35.
17. World Health Organization. WHO Guidelines on Safety Monitoring of Herbal Medicines in Pharmacovigilance Systems; 2004.
18. Wallace HM. Risk perception in toxicology-Part II: Toxicology must be the solution not the problem. Toxicol Sci 2011;121:7-11.
19. Dar SA, Ghazanfar K, Akbar S, Masood A, Nazir T, Siddiqui KM, et al. Acute and sub-acute oral toxicity studies of deedan-a unani drug in albino rats. J Appl Pharm Sci 2015;5:107-14.
202 Views | 66 Downloads
How to Cite
SASIKALA S, and KANNIKAPARAMESWARI N. “TOXICITY STUDIES OF A DEVELOPED NEPHROPROTECTIVE POLYHERBAL FORMULATION IN EXPERIMENTAL RATS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 6, Apr. 2019, pp. 166-9, doi:10.22159/ajpcr.2019.v12i6.32805.
Original Article(s)