ALTERING THE EXPRESSION OF P-GLYCOPROTEIN RESPONSIBLE FOR CHEMOTHERAPEUTIC DRUG RESISTANCE IN HEPG2 CELL LINE POST-TREATMENT WITH UREA AND β-MERCAPTOETHANOL

  • PROMA CHAKRABORTY Department of Biotechnology, Reva University, Bengaluru, Karnataka, India,
  • MANI RAMAKRISHNAN Department of Environmental Sciences, School of Engineering, Presidency University, Bengaluru, Karnataka, India,
  • KUMAR KM Department of Biotechnology, Reva University, Bengaluru, Karnataka, India,

Abstract

Objective: The objective of this study is to alter the expression of p-glycoprotein (p-gp) pump proteins in HepG2 cells after treating with urea and β-mercaptoethanol (BME) (lead compounds). The most common cause for resistance to a broad range of anticancer drugs is influenced by overexpression of p-gp pumps that detect and eject anticancer drugs from the cancer cell. Altering the expression of these proteins will reduce the efflux action and enhance the drug retention eventually killing the cancer cell.


Materials and Methods: 3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyl tetrazolium bromide (MTT) assay was carried out to measure the cell viability (HepG2 cells) post-treatment with the lead compounds followed by flow cytometric analysis for protein expression studies.


Results: MTT assay confirms that the viability of HepG2 cells reduces as the concentrations of the lead compounds are increased. Flow cytometric analysis confirms reduced p-gp expression in HepG2 cells post-treatment with urea and BME. Compare to BME, urea turns out to be a potential compound in altering the expression of p-gp.


Conclusion: The present cell line study confirms that urea and BME are potential compounds which are able to reduce the p-gp expression inHepG2 cells.

Keywords: HepG2 cells,, P-glycoprotein pumps,, Protein expression,, Urea, β-mercaptoethanol.

Author Biography

PROMA CHAKRABORTY, Department of Biotechnology, Reva University, Bengaluru, Karnataka, India,

School of Engineering

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PROMA CHAKRABORTY, MANI RAMAKRISHNAN, and KUMAR KM. “ALTERING THE EXPRESSION OF P-GLYCOPROTEIN RESPONSIBLE FOR CHEMOTHERAPEUTIC DRUG RESISTANCE IN HEPG2 CELL LINE POST-TREATMENT WITH UREA AND β-MERCAPTOETHANOL”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 6, May 2019, pp. 284-8, https://innovareacademics.in/journals/index.php/ajpcr/article/view/32834.
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