PROTECTIVE ROLE OF PTEROSTILBENE ON PLASMA AND TISSUE GLYCOPROTEIN COMPONENTS IN HIGH-FAT DIET-FED AND STREPTOZOTOCIN-INDUCED TYPE 2 DIABETIC MICE

  • GUNASEELAN THANGASAMY Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram, Tamil Nadu, India.
  • Gunaseelan Thangasamy, Mr Department of Biochemistry and Biotechnology, Faculty of Science, Annamalai University, Chidambaram, Tamil Nadu, India.

Abstract

Objective: The aim of the current study is to evaluate the effect of pterostilbene (PTS), on deranged plasma and tissue glycoprotein components in high-fat-diet (HFD) and streptozotocin (STZ)-induced type 2 diabetic mice.


Methods: The experimental duration was 16 weeks. The C57BL6/J mice were fed a normal diet, normal diet with PTS, HFD, and STZ injection (10th week only), and diabetic mice with PTS for the past 6 weeks.


Results: A significant increase in glycoprotein components such as hexose, hexosamine, fucose, and sialic acid (SA) in plasma was observed in diabetic mice. In hepatic and renal tissues, a significant decrease in SA with an increase in other glycoprotein components was detected in diabetic mice when compared with control mice. Oral administration of PTS significantly improved the glycoprotein levels in plasma and tissues of diabetic mice to near normal level.


Conclusion: In this study, we resolved that PTS improves disturbed glycoprotein metabolism in HFD and STZ-induced type 2 diabetic mice.

Keywords: Pterostilbene; High-fat diet; Streptozotocin; Hexosamine; Fucose; Sialic Acid

References

1. Nolan CJ, Damm P, Prentki M. Type 2 diabetes across generations: From pathophysiology to prevention and management. Lancet 2011;378:169 81.
2. Cho NH, Shaw JE, Karuranga S, Huang Y, da Rocha Fernandes JD, Ohlrogge AW, et al. IDF diabetes atlas: Global estimates of diabetes prevalence for 2017 and projections for 2045.Diabetes Res Clin Pract 2018;138:271-81.
3. Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care 2004;27:1047-53.
4. Asmat U, Abad K, Ismail K. Diabetes mellitus and oxidative stress a concise review. Saudi Pharm J 2016;2:547-53.
5. Liu Y, Sun J, Rao S, Su Y, Yang Y. Antihyperglycemic, antihyperlipidemic and antioxidant activities of polysaccharides from Catathelasma ventricosum in streptozotocin-induced diabetic mice. Food Chem Toxicol 2013;57:39-45.
6. Prakasam A, Sethupathy S, Pugalendi KV. Influence of Casearia esculenta root extract on glycoprotein components in streptozotocin diabetic rats. Pharmazie 2005:60:229-32.
7. Hidayati S, Sulistyawati R, Nurani LH. Regulation of ethyl acetate fraction from Moringa oleifera leaves to improve lipid metabolism and insulin sensitivity in Type 2 diabetes. Int J Pharm Pharm Sci 2018;10:78-82.
8. Senthilkumar GP, Subramanian SP. Biochemical studies on the effect of Terminalia chebulaon the levels of glycoproteins in streptozotocin-induced experimental diabetes in rats. J Appl Biomed 2008;6:105-15.
9. Saravananan G, Ponmurugana P, Kumar GP, Rajarajan T. Antidiabetic effect of S-allylcysteine: Effect on plasma and tissue glycoproteins in experimental diabetes. Phytomedicine 2010;17:1086-9.
10. Lin HS, Yue BD, Ho PC. Determination of pterostilbene in rat plasma by a simple HPLC-UV method and its application in pre-clinical pharmacokinetic study. Biomed Chromatogr 2009;23:1308-15.
11. Chakraborty A, Gupta N, Ghosh K, Roy P. In vitro evaluation of the cytotoxic, anti-proliferative and anti-oxidant properties of pterostilbene isolated from Pterocarpus marsupium. Toxicol In Vitro 2010;24:1215 28.
12. Kapetanovic IM, Muzzio M, Huang Z, Thompson TN, McCormick DL. Pharmacokinetics, oral bioavailability, and metabolic profile of resveratrol and its dimethylether analog, pterostilbene, in rats. Cancer Chemother Pharmacol 2011;68:593-601.
13. Niebes P. Determination of enzymes and degradation products of glycosaminoglycans metabolism in the serum of healthy and varicose subjects. Clin Chim Acta 1972;42:399-408.
14. Elson LA, Morgan WT. A colorimetric method for the determination of glucosamine and chondrosamine. Biochem J 1933;27:1824-8.
15. Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem 1959;234:1971-5.
16. Dische Z, Shettles LB. A specific color reaction of methyl pentoses and a spectrophotometric micro method for their determination. J Biol Chem 1948;175:596-603.
17. Widodo GP, Herowati R, Perangin-angin JM, Kamlasi JE. Antihyperglycemic, antioxidant, and pancreas regeneration activities of black cumin (Nigella sativa l.) seeds ethanol extract in alloxan-induced diabetic rats. Int J Pharm Pharm Sci 2016;8:37-40.
18. Brownlee M. The pathobiology of diabetic complications: A unifying mechanism. Diabetes 2005;54:1615-25.
19. Gemayel R, Fortpied J, Rzem R, Vertommen D, Veiga-da-Cunha M, Schaftingen VE. Many fructosamine 3-kinase homologues in bacteria are ribulosamine/erythrulosamine 3-kinases potentially involved in protein deglycation. FEBS J 2007;274:4360-74.
20. Pari L, Saravanan S. Thymol, a monoterpene phenolic compound ameliorates deranged glycoprotein metabolism in HFD-induced diabetic in C57BL/6J mice. Indo Asian J Multidiscip Res 2015;1:196 204.
21. Pari L, Karthikesan K. Protective role of tetrahydrocurcumin and chlorogenic acid on glycoprotein changes in streptozotocinnicotinamide-induced diabetic rats. J Pharm Sci Res 2009;1:173-80.
22. Ramkumar KM, Rajaguru P, Latha M, Ananthan R. Ethanol extract of Gymnema montanum leaves reduces glycoprotein components in experimental diabetes. Nutr Res 2007;27:97-103.
23. Cioffi DL, Pandey S, Alvarez DF, Cioffi EA. Terminal sialic acids are an important determinant of pulmonary endothelial barrier integrity. Am J Physiol Lung Cell Mol Physiol 2012;302:1067-77.
24. Prakash S, Sudha S. Relationship between nitric oxide and sialic acid concentrations in south Indian Type 2 diabetic patients. Adv Appl Sci Res 2013;4:258-62.
25. Rossi G, Paltrinieri S. Total sialic acid: An acute phase reactant in cats with a possible role in feline coronavirus infection. Can J Vet Res 2009;73:144-50.
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GUNASEELAN THANGASAMY, and LEELAVINOTHAN PARI. “PROTECTIVE ROLE OF PTEROSTILBENE ON PLASMA AND TISSUE GLYCOPROTEIN COMPONENTS IN HIGH-FAT DIET-FED AND STREPTOZOTOCIN-INDUCED TYPE 2 DIABETIC MICE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 12, no. 5, Apr. 2019, pp. 191-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/32940.
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